Difference between revisions of "Team:Bio Without Borders/InterLab"

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<b>Introduction</b>
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The interlab study was conducted to provide a standardized protocol for measurements of absorbance and fluorescence. We initially had trouble getting our Fluostar Galaxy to work as the program had certain constraints during the setup. Once we familiarized ourselves with how it functioned, we conducted the protocol using black 96 well plates. The first trial of the study was not accepted due to the fluorescence and absorbance readings being too similar between the test devices. The study was conducted once more, but with black wells and transparent bottom plates instead of using fully transparent plates. This submission gave outcomes closer to theoretical results, and was a successful submission.
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<b>Original Measurement Study</b>
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Calibration 1
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<img src="https://static.igem.org/mediawiki/2018/5/5b/T--Bio_Without_Borders--original_cal1.png">
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Calibration 2
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<img src="https://static.igem.org/mediawiki/2018/8/89/T--Bio_Without_Borders--original_cal_2.png">
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Calibration 3
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<img src="https://static.igem.org/mediawiki/2018/0/0a/T--Bio_Without_Borders--original_cal3.png">
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Fluorescence of Cell Cultures
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<img src="https://static.igem.org/mediawiki/2018/8/87/T--Bio_Without_Borders--original_fluo.png">
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Absorbance of Cell Cultures
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<img src="https://static.igem.org/mediawiki/2018/7/75/T--Bio_Without_Borders--original_abs.png">
  
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<b>Corrected Measurement Study</b>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<img src="https://static.igem.org/mediawiki/2018/1/1b/T--Bio_Without_Borders--redo_cal2.png">
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<h1>InterLab</h1>
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
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<img src="https://static.igem.org/mediawiki/2018/5/57/T--Bio_Without_Borders--redo_cal2_graph1.png">
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For teams participating in the <a href="https://2018.igem.org/Measurement/InterLab">InterLab study</a>, all work must be shown on this page.  
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Fluoresence Measurement
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<img src="https://static.igem.org/mediawiki/2018/e/e9/T--Bio_Without_Borders--redo_fluo.png">
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Absorbance Measurement
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<img src="https://static.igem.org/mediawiki/2018/e/e7/T--Bio_Without_Borders--redo_abs.png">
  
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Latest revision as of 19:10, 12 October 2018

Introduction
The interlab study was conducted to provide a standardized protocol for measurements of absorbance and fluorescence. We initially had trouble getting our Fluostar Galaxy to work as the program had certain constraints during the setup. Once we familiarized ourselves with how it functioned, we conducted the protocol using black 96 well plates. The first trial of the study was not accepted due to the fluorescence and absorbance readings being too similar between the test devices. The study was conducted once more, but with black wells and transparent bottom plates instead of using fully transparent plates. This submission gave outcomes closer to theoretical results, and was a successful submission.

Original Measurement Study

Calibration 1




Calibration 2







Calibration 3







Fluorescence of Cell Cultures



Absorbance of Cell Cultures



Corrected Measurement Study















Fluoresence Measurement



Absorbance Measurement