Difference between revisions of "Team:Bio Without Borders/InterLab"

 
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<b>Introduction</b>
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The interlab study was conducted to provide a standardized protocol for measurements of absorbance and fluorescence. We initially had trouble getting our Fluostar Galaxy to work as the program had certain constraints during the setup. Once we familiarized ourselves with how it functioned, we conducted the protocol using black 96 well plates. The first trial of the study was not accepted due to the fluorescence and absorbance readings being too similar between the test devices. The study was conducted once more, but with black wells and transparent bottom plates instead of using fully transparent plates. This submission gave outcomes closer to theoretical results, and was a successful submission.
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Calibration 2
 
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7/27 1:10 Dilution  iGEM Measurement Samples
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Calibration 3
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Fluorescence of Cell Cultures
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<img src="https://static.igem.org/mediawiki/2018/8/87/T--Bio_Without_Borders--original_fluo.png">
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Absorbance of Cell Cultures
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<b>Corrected Measurement Study</b>
  
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Fluoresence Measurement
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<img src="https://static.igem.org/mediawiki/2018/e/e9/T--Bio_Without_Borders--redo_fluo.png">
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Absorbance Measurement
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Latest revision as of 19:10, 12 October 2018

Introduction
The interlab study was conducted to provide a standardized protocol for measurements of absorbance and fluorescence. We initially had trouble getting our Fluostar Galaxy to work as the program had certain constraints during the setup. Once we familiarized ourselves with how it functioned, we conducted the protocol using black 96 well plates. The first trial of the study was not accepted due to the fluorescence and absorbance readings being too similar between the test devices. The study was conducted once more, but with black wells and transparent bottom plates instead of using fully transparent plates. This submission gave outcomes closer to theoretical results, and was a successful submission.

Original Measurement Study

Calibration 1




Calibration 2







Calibration 3







Fluorescence of Cell Cultures



Absorbance of Cell Cultures



Corrected Measurement Study















Fluoresence Measurement



Absorbance Measurement