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| | <html> | | <html> |
| − | <style type="text/css"> | + | <style> |
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| − | | + | #content { |
| − | /* Reset the wiki style by overriding with a reset stylesheet (written by Florian Albrecht)
| + | background: none; |
| − | the relevant wiki css rules are reset by using rules with higher priority */
| + | border: none; |
| − | | + | width: 100%; |
| − | | + | |
| − | /* Start general reset ================================================== */
| + | |
| − | | + | |
| − | body {
| + | |
| − | background-color: #C7E0F3;
| + | |
| − | background-image: url("https://static.igem.org/mediawiki/2018/b/b0/T--ASIJ_Tokyo--OfficialBackground.jpeg");
| + | |
| − | background-repeat: repeat, repeat;
| + | |
| − | background-size: 100%;
| + | |
| − | font-family: "Avenir";
| + | |
| − | padding: 0;
| + | |
| | } | | } |
| − | | + | #HQ page { |
| − | div#globalWrapper {
| + | display: hidden; |
| − | margin: 0;
| + | |
| − | padding: 0;
| + | |
| − | position: static;
| + | |
| − | width: auto;
| + | |
| − | background-color: transparent;
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| | } | | } |
| − | | + | .logo_2018 { |
| − | /* Start top-section reset ============================================== */
| + | margin-top: 0px; |
| − | | + | width: 0; |
| − | div#top-section {
| + | float: left; |
| − | border: 0;
| + | visibility: hidden; |
| − | height: auto; | + | |
| − | margin: 0;
| + | |
| − | position: static; | + | |
| − | width: auto; | + | |
| | } | | } |
| − | | + | #content H1 { |
| − | | + | visibility: hidden; |
| − | div#p-logo a, div#p-logo a:hover {
| + | |
| − | text-decoration: none; | + | |
| | } | | } |
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| − | div#search-controls {
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| − | background-color: transparent;
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| − | height: auto;
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| − | position: static;
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| − | right: 0;
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| − | text-align: left;
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| − | top: 0;
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| − | width: auto;
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| − | z-index: auto;
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| − | }
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| − | /* Start content reset ================================================== */
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| − | div#content {
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| − | background-color: transparent;
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| − | }
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| − | body li {
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| − | margin: 0;
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| − | }
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| − | body h2 {
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| − | font-family: 40px Avenir;
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| − | }
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| − | body h1, body h3, body h4, body h5, body h6 {
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| − | body .firstHeading {
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| − | margin: 0;
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| − | div#contentSub {
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| − | color: #f2b1b1;
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| − | font-size: inherit;
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| − | line-height: inherit;
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| − | margin: 0;
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| − | width: auto;
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| − | }
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| − | div#catlinks {
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| − | background-color: transparent;
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| − | border: 0;
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| − | clear: both;
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| − | margin: 0;
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| − | padding: 0;
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| − | }
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| − | div.container
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| − | {
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| − | font-family: Avenir;
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| − | font-weight: bold;
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| − | position: relative;
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| − | margin: 0 auto;
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| − | padding: 5em 3em;
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| − | text-align: center;
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| − | }
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| − | div.container a
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| − | {
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| − | color: #1e50a2;
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| − | text-decoration: none;
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| − | font: 20px Avenir;
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| − | margin: 0px 10px;
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| − | padding: 10px 10px;
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| − | position: relative;
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| − | z-index: 0;
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| − | cursor: pointer;
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| − | }
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| − | div.topBotomBordersOut a:before, div.topBotomBordersOut a:after
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| − | {
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| − | position: absolute;
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| − | left: 0px;
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| − | width: 100%;
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| − | height: 2px;
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| − | background: #1e50a2;
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| − | content: "";
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| − | opacity: 0;
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| − | transition: all 0.3s;
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| − | }
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| − | div.topBotomBordersOut a:before
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| − | {
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| − | top: 3px;
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| − | transform: translateY(10px);
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| − | div.topBotomBordersOut a:after
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| − | {
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| − | bottom: 3px;
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| − | transform: translateY(-10px);
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| − | }
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| − | div.topBotomBordersOut a:hover:before, div.topBotomBordersOut a:hover:after
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| − | {
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| − | opacity: 1;
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| − | transform: translateY(0px);
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| − | }
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| − | /* Start footer reset =================================================== */
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| − | div#footer-box {
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| − | position: relative;
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| − | padding: 1rem;
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| − | width: 100%;
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| − | border-color: #E8E6E6;
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| − | background-color: transparent;
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| − | }
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| − | div#footer {
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| − | left: 45%;
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| − | top: -20%;
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| − | padding: 20px;
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| − | margin: 0 auto;
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| − | div#f-poweredbyico, div#f-copyrightico {
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| − | float: none;
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| − | #sideMenu{
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| − | width: 200px;
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| − | position: absolute;
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| − | top: 20px;
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| − | left: 1020px;
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| − | z-index: 10;
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| − | padding-top: 0px;
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| − | padding-bottom: 15px;
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| − | padding-left: 15px;
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| − | padding-right: 15px;
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| − | text-align: left;
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| − | display: none;
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| − | z-index: 9996;
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| − | }
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| − | #content{
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| − | width: 100%;
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| − | padding: 0px;
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| − | margin-left: 0px;
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| − | }
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| − | #top_title{
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| − | overflow: hidden;
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| − | display: none;
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| − | }
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| − | #top_menu_14{
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| − | height: 20px;
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| − | }
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| − | #top_menu_under{
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| − | height: 0px;
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| − | }
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| − | #globalWrapper{
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| − | padding-bottom: 20px;
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| − | }
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| − | #HQ_page p {
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| − | font-size: 16px;
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| − | }
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| − |
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| | </style> | | </style> |
| − | <body>
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| − |
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| − | <div class="container topBottomBordersOut">
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| − | <a href="https://2018.igem.org/Team:ASIJ_Tokyo">HOME</a>
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| − | <a href="https://2018.igem.org/Team:ASIJ_Tokyo/Team">TEAM</a>
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| − | <a href="#">PROJECT</a>
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| − | <a href="https://2018.igem.org/Team:ASIJ_Tokyo/Parts">PARTS</a>
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| − | <a href="https://2018.igem.org/Team:ASIJ_Tokyo/Engagement">HUMAN PRACTICES</a>
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| − | <a href="https://2018.igem.org/Team:ASIJ_Tokyo/Safety">SAFETY</a>
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| − | </div>
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| − | <div id="content">
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| − | <div id="title">
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| − | <p>A1-AT<br>de<span id="red">LIVER</span>y </p>
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| − | <img src="https://static.igem.org/mediawiki/2018/e/ed/T--ASIJ_Tokyo--liverlogocut.png" id="liver">
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| − | </div>
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| − | <div id="subtitle">
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| − | <h1> Using Stem Cell and CRISPR Technology to Combat <br> Alpha-1 Antitrypsin Deficiency </h1>
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| − | </div>
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| − | <div id="abstract">
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| − | <h3> ABSTRACT </h3>
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| − | <p> Alpha-1 Antitrypsin deficiency is a common genetic disorder -- the defective gene for which is carried by 1 in 25 people -- which arises from a single base pair mutation in the SERPINA1 gene, resulting in the production of a form of antitrypsin prone to polymerization. The mutated antitrypsin then builds up in liver cells and is unable to inhibit proteases in the lungs, leading to damage in both. Using CRISPR-Cas9 technology, we aimed to fix the error in SERPINA1 so that proper antitrypsin can be produced. We will show proof of concept in E. Coli cells using osmy secretion tags and GFP as a reporter. We hope to design a liver organ bud using IPS cell technology to deliver function A1AT through collaboration with Dr. Kagimoto of Healios Japan KK.
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| − | </div>
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| − | </div>
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| − |
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| − | <div id="footer-box">
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| − | <div id="footer">
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| − | <img src="https://static.igem.org/mediawiki/2018/9/9f/T--ASIJ_Tokyo--logo.png" width="150px"> </img>
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| − | </div>
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| − | </div>
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| − | </body>
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| | </html> | | </html> |
