Difference between revisions of "Team:AHUT China/HP FOR SILVER"

    As the project continues to advance, HP's exchanges and cooperations between teams are more frequent and in-depth. After seeing the cooperation application issued by the iGEM team of the Indian Institute of Technology (IIT-Madras) at the iGEM official website, we contacted IIT-Madras to discuss cooperation actively.
  We are pleased to collaborate with the iGEM team of the Indian Institute of Technology, Madras (IIT-Madras) to make a video on the science of synthetic biology in the mother tongue. The Indian Institute of Technology is also working with other teams in English, Tamil, Telugu, Kannada, Malayalam, Marathi, Bengali, Gujarati and Hindi.
          IIT-Madras sends an English script to our team. We need to match the provided slideshow video with the provided text and add the translated text to the subtitles, and finally make it into Chinese video.
          We thought it was a simple matter, but there were some problems at the beginning. The problem is summarized as follows:
          1. The English script does not exactly match the sound in the video, and some paragraphs are missing.
         2. English and Chinese belong to different language families. They are slightly different in grammar, and the word order is reversed in translation.
          3. Some video pictures and sounds are out of sync and need to be adjusted.
          4. Our team members are not familiar enough with the Indian accent in English, and some words cannot be completely accurate.
          After consultation and repeated revisions by the iGEM team members of the Anhui University of Technology, four days later, the results were satisfactory and the video was sent to the iGEM team of the Indian Institute of Technology. We have established a good relationship with the iGEM team (IIT-Madras) of the Indian Institute of Technology, Madras.
With the successful completion of the HP cooperation, the Anhui University of Technology iGEM team (AHUT-CHINA) looks forward to more cooperation opportunities with the Indian Institute of Technology iGEM team (IIT-Madras).

          On the afternoon of September 12, 2018, our team AHUT_China visited Maanshan Hua Qi Environmental Protection Technology Co., Ltd. (Hereinafter referred to as HuaQi Environmental Protection Company).

          We know that environmental pollution is mainly air pollution, water pollution and soil pollution. Our project is to deal with greenhouse gas CO2 in air pollution. How to deal with greenhouse gas CO2 efficiently is particularly critical as the increasing greenhouse effect has a serious impact on the global environment. In order to gain a deeper understanding of the current environmental pollution in China, we visited HuaQi Environmental Protection Company. HuaQi Environmental Protection Company, is a company specializing in sewage treatment. After Ms. Zhang Qi introduced the company, we visited the intelligent water transportation management system and laboratory.

          Liu Shanshan, the company's technical staff, led us to visit the laboratory. During the visit, we learned that water pollution currently is harmful to the environment and people's health. So, water pollution treatment is very important. We introduced our project to computerize the protein molecules expressed by carbonic anhydrase gene mutations to obtain carbonic anhydrase (CA-2) with high thermal stability and import it into E. coli cells in order to achieve efficient collection of CO2. The project is currently in the theoretical stage. She is interested in our project. If the CO2 emission can be effectively controlled at the source, the problems such as the continued destruction of ozone layer, the melting of two-stage glaciers and the rapid extinction of a large number of species can be effectively solved. And she is more concerned about how this project can be applied in practice, and puts forward some suggestions for our project. At present, the CO2 gas capture technology mainly includes chemical absorption, pressure swing adsorption and membrane separation. She suggested first that transgenic E. coli be cultured in solution environment with chemical absorption to achieve CO2 collection, and secondly that CO2 be treated with biological rotating disc.

         We saw the cooperation request issued by Linking University's iGEM team (Linkoping_Sweden). Linkoping_Sweden has a new idea of cooperation. They want other teams to use letters or emails to show interesting things happening inside and outside the team. So we participated in this cooperation. This is an interesting collaboration, as it may be boring during the experiment, but the stories that happen in the experiment make the experiment fun. And by organizing these stories, we learn more about our experiments.

          September 9, 2018, We arranged a video conference with members from northeastern university, Shandong University and northwestern University. During this time, we briefly introduced our project background and project ideas and planned the agenda of the Meeting. As the greenhouse effect has become a common concern in recent years, how to effectively capture carbon dioxide has become a worldwide problem. At present, carbon dioxide capture mainly includes absorption, adsorption and membrane method, these are high cost, renewable energy consumption and two times pollution problems. The use of CO2 to capture the high catalytic efficiency and environmental friendliness of the carbon anhydride has aroused wide concern,

  

          So our Anhui University of Technology team put forward a project on the generation of carbon anhydride through E. coli to absorb carbon dioxide. First of all, our project successfully expressed the Wide-type carbonic anhydride enzyme in Escherichia coli, however, due to its poor stability and ease of deactivation, its industrial applications were limited. Therefore, based on the molecular simulation technique, the effect of mutation of amino acid residues on the conformation and activity of enzymes was studied, and the mutant carbonic anhydride with high thermal stability was Obtained. The experimental results show that the purified mutant carbonic anhydride has higher stability and activity than the wild type of carbonic anhydride enzyme, which realizes the effective capture of CO2. Northeastern university, Shandong university, The members of Northwestern University have expressed their strong interest in our team project of Anhui University of technology, so we have the highlights of our project and other members of the school exchange, Highlights: 1, relative to the high cost, high efficiency and poor circulation of traditional methods, we choose high catalytic efficiency, low cost, A safe and environmentally friendly method for trapping CO2 with carbon anhydride. 2, using the molecular simulation technique, the amino acid as the basic unit, the effect of remnant radical mutation on the two-stage structure of the carbonic anhydride enzyme, and the influence of the molecular conformation, the best amino acid mutation site was obtained, and the thermal stability of the enzyme was improved without affecting the enzyme Activity. 3, we obtained the expression of wild type and mutant carbonic anhydride enzyme strains of Escherichia coli, and two kinds of carbon anhydride enzyme in vitro purification, wild-type carbonic anhydride enzyme has a certain CO2 capture efficiency, more importantly, the mutant than the wild type of carbonic anhydride enzyme has better stability and activity, realize the CO2 efficient capture.

     Students from Tohoku University also briefed members of our other schools about their projects, and the Tohoku University project was inspired by the prevalence of fast food and the acceleration of urbanization, with the number of inflammatory bowel diseases (inflammatory bowel disease, IBD) Rising in asia, And the risk of developing colorectal cancer (colorectal cancer, CRC) in patients with IBD also increased. There are no drugs that are better for ibd, so they want to use engineered probiotics to treat IBD and prevent crc, using synthetic biology. The rest of us were very interested in the project, and our Anhui University of Technology team actively asked them for details about their project, and they briefed them on the project background and the project brief. The background of their project is that inflammatory bowel disease (IBD) is caused by inappropriate immune responses, which in general trigger a series of events that can disrupt the barrier when the immune system reacts excessively to the bacteria in the gut, eventually causing IBD when the immune imbalance persists without intervention. There is no cure for IBD at present, the best treatment now is to use anti-inflammatory drugs such as TNF inhibitor treatment, but the Patient's drug treatment effect is huge difference, to more than 50% of patients Ineffective. Probiotics are beneficial to the host of active microorganisms, colonization in the human intestinal tract, reproductive system, oral cavity and so can produce the exact health effects of the microbial group, has been beneficial to bacteria such as boulardii yeast used in clinical diarrhea Treatment. The Tohoku University team aims to design a biological system for ANTI-IBD and CRC prevention through the use of engineered Probiotics. Because the concentration of no in the intestinal tract of IBD is about 100 times times that of normal people, no molecule acts as their inflammatory signaling molecule, and probiotic Escherichia coli (nissle 1917) is selected as their chassis organism. The project consists of two sets of devices, one of which is an anti-inflammatory device, which includes a sensor for detecting inflammatory signals, a high-efficiency enhancer, and an effector that secretes interleukin (IL-10); the second is an anti-cancer device, which is expressed through engineered probiotics, which is derived from a cross-flowered vegetable ( Cruciferous vegetables (myrosinase), which converts the natural component of Thio-glucoside (sinigrin) from a cross-flowered vegetable into a turnip (sulforaphane), The product of radish is a kind of organic molecule with anticancer activity, and it can inhibit oxidative stress (oxidative stress), so it can also inhibit the intestinal inflammatory reaction. In addition, they use cold-shock kill switches (cold-excited expression lysis and Maz-ef) to prevent their engineered bacteria from escaping into the environment and causing DNA contamination.

    Members of the Northwestern University team introduced their project background that saline soils may affect the growth of crops such as wheat and rice. Because traditional saline soils are difficult and inefficient, they have decided to address this global challenge in new ways. Several parts of the bacterium are designed to reduce the concentration within the Range. Their project is to modify potassium channels by using non-natural amino acid d-alanine, they obtained a new channel protein, which allows K + to be considered in a high concentration of K + and allows Na + to be considered in a low concentration of K +. Because of the use of d-ala, a part of the introduction of unnatural amino acids into bacteria was established. They analyzed proteins and tested them by molecular dynamics simulations and quantum chemistry, and established mathematical models of the relationship between cells and salt concentrates. They will use patch clamps and a new method to test proteins and bacteria in the Solution. After their cell experiments, they will use it for agricultural and water purification processes.

     The Shandong University team project is about PHB an environmental bio-plastic Project. At the same time, we will carefully analyze the problems in the experiments and modeling that are currently encountered in our project, so that we can better understand our project and excavate the parts that we may need to cooperate. In the course of the discussion, we talked about our understanding of synthetic biology, including its great potential for human benefit and the recent astonishing breakthroughs scientists have made. Then we showed and explained our project in depth, and they gave us some good suggestions, such as the improvements in cell secretion pathways that are practical, valuable and Meaningful. We are really looking forward to our late-stage cooperation and exchanges with the United States to participate in the event of grand camping meetings.