Difference between revisions of "Team:TUDelft/Primers/Main"
Nicolebennis (Talk | contribs) |
Nicolebennis (Talk | contribs) |
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| Line 33: | Line 33: | ||
<table> | <table> | ||
| − | |||
<tr class="tableheadergrnbl"> | <tr class="tableheadergrnbl"> | ||
<th class="tableheadergrnbl">ID</th> | <th class="tableheadergrnbl">ID</th> | ||
| Line 631: | Line 630: | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
<td>099</td> | <td>099</td> | ||
| − | <td></td> | + | <td>fw_BB_sgRNA_EPO1</td> |
| − | <td></td> | + | <td>BioBrick</td> |
| − | <td></td> | + | <td>gaattcgcggccgcttctagagGGAGGCCGAGAATATCAC</td> |
</tr> | </tr> | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
<td>100</td> | <td>100</td> | ||
| − | <td></td> | + | <td>fw_BB_sgRNA_EPO2</td> |
| − | <td></td> | + | <td>BioBrick</td> |
| − | <td></td> | + | <td>gaattcgcggccgcttctagagACAGCCCGTCGTGATATTCT</td> |
</tr> | </tr> | ||
| − | + | <tr class="tableunevengrnbl"> | |
<td>101</td> | <td>101</td> | ||
| + | <td>fw_BB_T7_sgRNA</td> | ||
| + | <td>BioBrick</td> | ||
| + | <td>gaattcgcggccgcttctagagaagcTAATACGACTCACTATAGG</td> | ||
| + | </tr> | ||
| + | <tr class="tableevengrnbl"> | ||
| + | <td>102</td> | ||
| + | <td>rv_BB_sgRNA</td> | ||
| + | <td>BioBrick</td> | ||
| + | <td>ctgcagcggccgctactagtaGCACCGACTCGGTGCCAC</td> | ||
| + | </tr> | ||
| + | <tr class="tableevengrnbl"> | ||
| + | <td>103</td> | ||
| + | <td>SeqTim_01</td> | ||
| + | <td>Sequencing</td> | ||
| + | <td>GATATGAATAAATTGCAGTTTGTCGGGCAGCAGGCCGTAGA</td> | ||
| + | </tr> | ||
| + | <tr class="tableunevengrnbl"> | ||
| + | <td>104</td> | ||
| + | <td>SeqTim_02</td> | ||
| + | <td>Sequencing</td> | ||
| + | <td>CATCATTGGCAACGCTACCCTCCATCCTCTTCCAGGCAT</td> | ||
| + | </tr> | ||
| + | <tr class="tableevengrnbl"> | ||
| + | <td>105</td> | ||
| + | <td>SeqTim_03</td> | ||
| + | <td>Sequencing</td> | ||
| + | <td>TCTACGGCCTGCTGCCCGACAAACTGCAATTTATTCATATC</td> | ||
| + | </tr> | ||
| + | <tr class="tableunevengrnbl"> | ||
| + | <td>106</td> | ||
| + | <td>SeqTim_04</td> | ||
| + | <td>Sequencing</td> | ||
| + | <td>ATGCCTGGAAGAGGATGGAGGGTAGCGTTGCCAATGATG</td> | ||
| + | </tr> | ||
| + | <tr class="tableevengrnbl"> | ||
| + | <td>107</td> | ||
| + | <td>SeqTim_05</td> | ||
| + | <td>Sequencing</td> | ||
| + | <td>TTCGGGCTCTGGGAGCCCAGccgcctccatccagtctatt</td> | ||
| + | </tr> | ||
| + | <tr class="tableunevengrnbl"> | ||
| + | <td>108</td> | ||
| + | <td>SeqTim_06</td> | ||
| + | <td>Sequencing</td> | ||
| + | <td>GGAGGGGAGATGGCTTCCTTgaatgaagccataccaaacg</td> | ||
| + | </tr> | ||
| + | <tr class="tableevengrnbl"> | ||
| + | <td>109</td> | ||
| + | <td>SeqTim_07</td> | ||
| + | <td>Sequencing</td> | ||
| + | <td>cgtttggtatggcttcattcAAGGAAGCCATCTCCCCTCC</td> | ||
| + | </tr> | ||
| + | <tr class="tableunevengrnbl"> | ||
| + | <td>110</td> | ||
| + | <td>SeqTim_08</td> | ||
| + | <td>Sequencing</td> | ||
| + | <td>aatagactggatggaggcggCTGGGCTCCCAGAGCCCGAA</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <table> | ||
| + | <tr class="tableheadergrnbl"> | ||
| + | <th class="tableheadergrnbl">ID</th> | ||
| + | <th class="tableheadergrnbl">Name</th> | ||
| + | <th class="tableheadergrnbl">Module</th> | ||
| + | <th class="tableheadergrnbl">Sequence (5' to 3')</th> | ||
| + | </tr> | ||
| + | <tr class="tableunevengrnbl"> | ||
| + | <td>001</td> | ||
<td>fw_IV1</td> | <td>fw_IV1</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 648: | Line 719: | ||
</tr> | </tr> | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
| − | <td> | + | <td>002</td> |
<td>rv_IV1</td> | <td>rv_IV1</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 654: | Line 725: | ||
</tr> | </tr> | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
| − | <td> | + | <td>003</td> |
<td>fw_IV2</td> | <td>fw_IV2</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 660: | Line 731: | ||
</tr> | </tr> | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
| − | <td> | + | <td>004</td> |
<td>rv_IV2</td> | <td>rv_IV2</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 666: | Line 737: | ||
</tr> | </tr> | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
| − | <td> | + | <td>005</td> |
<td>fw_KanR</td> | <td>fw_KanR</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 672: | Line 743: | ||
</tr> | </tr> | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
| − | <td> | + | <td>006</td> |
<td>rv_KanR</td> | <td>rv_KanR</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 678: | Line 749: | ||
</tr> | </tr> | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
| − | <td> | + | <td>007</td> |
<td>fw_diagnostic</td> | <td>fw_diagnostic</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 684: | Line 755: | ||
</tr> | </tr> | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
| − | <td> | + | <td>008</td> |
<td>rv_diagnostic</td> | <td>rv_diagnostic</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 690: | Line 761: | ||
</tr> | </tr> | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
| − | <td> | + | <td>009</td> |
<td>fw_KanRCassetteME</td> | <td>fw_KanRCassetteME</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 696: | Line 767: | ||
</tr> | </tr> | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
| − | <td> | + | <td>010</td> |
<td>rv_KanRCassetteME</td> | <td>rv_KanRCassetteME</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 702: | Line 773: | ||
</tr> | </tr> | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
| − | <td> | + | <td>011</td> |
<td>fw_KanRCassette</td> | <td>fw_KanRCassette</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 708: | Line 779: | ||
</tr> | </tr> | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
| − | <td> | + | <td>012</td> |
<td>rv_KanRCassette</td> | <td>rv_KanRCassette</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 714: | Line 785: | ||
</tr> | </tr> | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
| − | <td> | + | <td>013</td> |
<td>fw_KanRCassetteME</td> | <td>fw_KanRCassetteME</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 720: | Line 791: | ||
</tr> | </tr> | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
| − | <td> | + | <td>014</td> |
<td>rv_KanRCassetteME</td> | <td>rv_KanRCassetteME</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 726: | Line 797: | ||
</tr> | </tr> | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
| − | <td> | + | <td>015</td> |
<td>fw_KanRCassette</td> | <td>fw_KanRCassette</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 732: | Line 803: | ||
</tr> | </tr> | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
| − | <td> | + | <td>016</td> |
<td>rv_KanRCassette</td> | <td>rv_KanRCassette</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 738: | Line 809: | ||
</tr> | </tr> | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
| − | <td> | + | <td>017</td> |
<td>fw_KanRMEBiobrick</td> | <td>fw_KanRMEBiobrick</td> | ||
<td>BioBrick</td> | <td>BioBrick</td> | ||
| Line 744: | Line 815: | ||
</tr> | </tr> | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
| − | <td> | + | <td>018</td> |
<td>rv_KanRMEBiobrick</td> | <td>rv_KanRMEBiobrick</td> | ||
<td>BioBrick</td> | <td>BioBrick</td> | ||
| Line 750: | Line 821: | ||
</tr> | </tr> | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
| − | <td> | + | <td>019</td> |
<td>rv_KanR internal</td> | <td>rv_KanR internal</td> | ||
<td>BioBrick</td> | <td>BioBrick</td> | ||
| Line 756: | Line 827: | ||
</tr> | </tr> | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
| − | <td> | + | <td>020</td> |
<td>fw_KanR internal</td> | <td>fw_KanR internal</td> | ||
<td>BioBrick</td> | <td>BioBrick</td> | ||
| Line 762: | Line 833: | ||
</tr> | </tr> | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
| − | <td> | + | <td>021</td> |
<td>fw target seq LacZ</td> | <td>fw target seq LacZ</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 768: | Line 839: | ||
</tr> | </tr> | ||
<tr class="tableevengrnbl"> | <tr class="tableevengrnbl"> | ||
| − | <td> | + | <td>022</td> |
<td>rv target seq LacZ</td> | <td>rv target seq LacZ</td> | ||
<td></em>In Vivo</em></td> | <td></em>In Vivo</em></td> | ||
| Line 774: | Line 845: | ||
</tr> | </tr> | ||
<tr class="tableunevengrnbl"> | <tr class="tableunevengrnbl"> | ||
| − | <td> | + | <td>023</td> |
<td>fw_pSB1C3ori</td> | <td>fw_pSB1C3ori</td> | ||
<td>BioBrick</td> | <td>BioBrick</td> | ||
<td>GCAAGCAGCAGATTACGCG</td> | <td>GCAAGCAGCAGATTACGCG</td> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
| + | |||
</div> | </div> | ||
Revision as of 13:53, 13 October 2018
stuff to write here
Primers
| ID | Name | Module | Sequence (5' to 3') |
|---|---|---|---|
| 001 | fw_dxCas9 | Fusion Protein | atataGCGGCCGCtatggacaagaagtactccat |
| 002 | rv_dxCas9 | Fusion Protein | tatGGTACCttatGGCCGGCCcaccttcctcttcttcttgg |
| 003 | fw_dxCas9_mut_EcoRI | Fusion Protein | P-gcgaattttccaaaagagtgatcc |
| 004 | rv_dxCas9_mut_EcoRI | Fusion Protein | P-ttatttgctcgatgatctcatcaagg |
| 005 | fw_Tn5_afterC | Fusion Protein | tataGGCCGGCCtAAACTGGGCGGCGGCGCGCCGGCGGTGGGCGGCGGCCCGAAAGCGGCGGATAAAatgattaccagtgcactgcatc |
| 006 | rv_Tn5_afterC | Fusion Protein | tataGGTACCttagattttaatgccctgcgcca |
| 007 | fw_Tn5_beforeC | Fusion Protein | tataGAATTCtatgattaccagtgcactgcatc |
| 008 | rv_Tn5_beforeC | Fusion Protein | tataGCGGCCGCTTTATCCGCCGCTTTCGGGCCGCCGCCCACCGCCGGCGCGCCGCCGCCCAGTTTgattttaatgccctgcgcca |
| 009 | rv_dxCas9_mut1_GA | Fusion Protein | cattactaaagatctcttgcaggtagca |
| 010 | fw_dxCas9_mut1_GA | Fusion Protein | gctacctgcaagagatctttagtaatg |
| 011 | rv_dxCas9_mut2_GA | Fusion Protein | gaccttaacggtttgcagtattcc |
| 012 | fw_dxCas9_mut2_GA | Fusion Protein | ggaatactgcaaaccgttaagg |
| 013 | rv_dxCas9_mut3_GA | Fusion Protein | ccgttttgcaggtagtacag |
| 014 | fw_dxCas9_mut3_GA | Fusion Protein | ctgtactacctgcaaaacgg |
| 015 | rv_dxCas9_mut4_GA | Fusion Protein | tcgttacctttttgcagcac |
| 016 | fw_dxCas9_mut4_GA | Fusion Protein | tgctgcaaaaaggtaacgag |
| 017 | rv_dxCas9_mut5_GA | Fusion Protein | tacttgaaggctgccggc |
| 018 | fw_dxCas9_mut_GA | Fusion Protein | gccggcagccttcaag |
| 019 | fw_dxCas9_mut1_BL | Fusion Protein | P-gctacctgcaagagatctttagtaatg |
| 020 | rv_dxCas9_mut1_BL | Fusion Protein | P-agatccgattctttctgcgg |
| 021 | fw_dxCas9_mut2_BL | Fusion Protein | P-ggaatactgcaaaccgttaagg |
| 022 | rv_dxCas9_mut2_BL | Fusion Protein | P-ctttttgatagctgggctacct |
| 023 | fw_dxCas9_mut3_BL | Fusion Protein | P-ctgtactacctgcaaaacgg |
| 024 | rv_dxCas9_mut3_BL | Fusion Protein | P-gtagagcttctcattctgaagc |
| 025 | fw_dxCas9_mut4_BL | Fusion Protein | P-ctgcaaaaaggtaacgagctg |
| 026 | rv_dxCas9_mut4_BL | Fusion Protein | P-cacgcccgcactagc |
| 027 | fw_dxCas9_mut5_BL | Fusion Protein | P-gccggcagccttcaag |
| 028 | rv_dxCas9_mut5_BL | Fusion Protein | P-gcgcccaagttggtca |
| 029 | fw_T7_promoter | General | TAATACGACTCACTATAGGG |
| 030 | rv_T7_promoter | General | GCTAGTTATTGCTCAGCGG |
| 031 | VF2 | General | tgccacctgacgtctaagaa |
| 032 | VR | General | attaccgcctttgagtgagc |
| 033 | fw_sgRNA_1 | Fusion Protein | aagcTAATACGACTCACTATAGGGGAGGCCGAGAATATCA |
| 034 | rv_sgRNA_1 | Fusion Protein | AAAAGCACCGACTCGGTGCCACTT |
| 035 | fw_sgRNA_2 | Fusion Protein | aagcTAATACGACTCACTATAGGACAGCCCGTCGTGATATTCTGTTTTAGAGCTAGAAATAG |
| 036 | fw_adTn5_1 | Fusion Protein | CTAGCTGTCTCTTATACACATCTATTGGCGAAATGGATGAACTGTTTACCATGC |
| 037 | rv_adTn5_1 | Fusion Protein | GCATGGTAAACAGTTCATCCATTTCGCCAATAGATGTGTATAAGAGACAGCTAG |
| 038 | fw_1_AUpart | d-AuNPs | GATCGAATTCGCGGCCGCTTC |
| 039 | rv_1_AUpart | d-AuNPs | CGATTCTGCAGCGGCCGCTAC |
| 040 | fw_2_AUpart | d-AuNPs | GGAGGCCGAGAATATCACGA |
| 041 | rv_2_AUpart | d-AuNPs | TTGAGATCGTTTTGGTCTGC |
| 042 | fw_3_AUpart | d-AuNPs | CAGGTCGACAAGCTTGCG |
| 043 | fw_dCas9 | Fusion Protein | atataGCGGCCGCtatggataagaaatactcaataggcttagc |
| 044 | rv_dCas9 | Fusion Protein | tatGGTACCttatGGCCGGCCgtcacctcctagctgactc |
| 045 | fw_1_Tn5-L-dxCas9_Gblock | Fusion Protein | ACCATGGGCAGCAGC |
| 046 | rv_1_Tn5-L-dxCas9_Gblock | Fusion Protein | GTAGGGTATCCGAAATGTGAGGATTTTC |
| 047 | fw_2_Tn5-L-dxCas9_Gblock | Fusion Protein | GGGAAAAGATTGAGAAAATCCTCACATTTCG |
| 048 | rv_2_Tn5-L-dxCas9_Gblock | Fusion Protein | TGGCGTTCAAATTTCGCAGC |
| 049 | fw_pACYC-Duet_Backbone | Fusion Protein | GGTACCCTCGAGTCTGG |
| 050 | rv_pACYC-Duet_Backbone | Fusion Protein | GAATTCGGATCCTGGCTG |
| 051 | fw_BB_pSB1C3 | BioBrick | TACTAGTAGCGGCCGCTGC |
| 052 | rv_BB_pSB1C3 | BioBrick | CTAGAAGCGGCCGCGA |
| 053 | rv_BB_pSB1C3_extraNuc | BioBrick | CTCTAGAAGCGGCCGCGA |
| 054 | fw_BB_Tn5 | BioBrick | GCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGatgattaccagtgcactgcatcg |
| 055 | rv_BB_Tn5 | BioBrick | CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAttagattttaatgccctgcgccatc |
| 056 | fw_BB_dxCas9 | BioBrick | GCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGatggacaagaagtactccattgg |
| 057 | rv_BB_dxCas9 | BioBrick | CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAttatggccggcccac |
| 058 | fw_BB_Linker | BioBrick | CGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGCCGGCCtAAACTGG |
| 059 | rv_BB_Linker | BioBrick | CTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTTATCCGCCGCTTTCGGGCCGCCGC |
| 060 | fw_BB_T7p | BioBrick | ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGGAATTGTG |
| 061 | rv_BB_T7p | BioBrick | TTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAGCTAGTTATTGCTCAGCGGTG |
| 062 | fw_adTn5_2 | Fusion Protein | CTGTCTCTTATACACATCTATTGGCGAAATGGATGAACTGTTTACCATGC |
| 063 | rv_adTn5_2 | Fusion Protein | GCATGGTAAACAGTTCATCCATTTCGCCAATAGATGTGTATAAGAGACAG |
| 064 | rv_adTn5_label | Fusion Protein | Cy-GCATGGTAAACAGTTCATCCATTTCGCCAATAGATGTGTATAAGAGACAG |
| 065 | fw_1_AUpart | d-AuNPs | |
| 066 | fw_bovine_albumin | Sample Preparation | TTGTTTCAACTCAAACAGCCTT |
| 067 | rv_bovine_albumin | Sample Preparation | AGAATCCCCATGGACAGAGA |
| 068 | fw_vectorxcas_spike | Sample Preparation | AGTAGCGGCCGCTGCAGaaggaagaggctgcaatctgtggc |
| 069 | rv_vectorxcas_spike | Sample Preparation | AGAAGCGGCCGCGAATTCcacacaagcagggagcagatactgg |
| 070 | fw_epo_spike | Sample Preparation | tgctccctgcttgtgtgGAATTCGCGGCCGCTTCT |
| 071 | rv_epo_spike | Sample Preparation | gattgcagcctcttccttCTGCAGCGGCCGCTAC |
| 072 | fw_epo_frag | Sample Preparation | GCCTCCCAGTCCTGGGCG |
| 073 | rv_epo_frag | Sample Preparation | GCAGTTGCAGGGGCTCCCAC |
| 074 | fw_epo_check | Sample Preparation | CCTGGAGAGGTACCTCTTGGAGGC |
| 075 | rv_epo_check | Sample Preparation | GCCCGACCTCCATCCTCTTCCAG |
| 076 | rv_adKan150 | Fusion Protein | catcctatggaactgcctcggtgagttttctccttc |
| 077 | rv_adKan300 | Fusion Protein | gaaatgcataagcttttgccattctcaccggattc |
| 078 | rv_adKan800 | Fusion Protein | gagccatattcaacgggaaacgtcttgctcc |
| 079 | fw_3_dxCas9-L-Tn5_Gblock | Fusion Protein | agaaataattttgtttaactttaataaggagatataccatgggc |
| 080 | rv_3_dxCas9-L-Tn5_Gblock | Fusion Protein | CTCGAATCAGTTTGTCATTTTCATCGTACTTG |
| 081 | fw_4_dxCas9-L-Tn5_Gblock | Fusion Protein | CCCAAATTCTCGATTCACGCATG |
| 082 | rv_4_dxCas9-L-Tn5_Gblock | Fusion Protein | tttagaggccccaaggggttatg |
| 083 | fw_2pACYC-Duet_Backbone | Fusion Protein | CTGCTGCCACCGCTG |
| 084 | rv_2pACYC-Duet_Backbone | Fusion Protein | GCTGCTGCCCATGGTATATC |
| 085 | fw_gblock1_mut_EcoRI | Fusion Protein | CAGGATCCGAATACTATGATTACC |
| 086 | rv_gblock1_mut_EcoRI | Fusion Protein | GGTAATCATAGTATTCGGATCCTG |
| 087 | fw_adTn5_Phos | Fusion Protein | P-CTGTCTCTTATACACATCT |
| 088 | fw_1_Tn5-dxCas9_Gblock_option2 | Fusion Protein | accatgggcagcagccatcaccatcatcaccacagcc |
| 089 | rv_1_Tn5-dxCas9_Gblock_option2 | Fusion Protein | cagggaaaagattgagaaaatcctcacatttcggataccctac |
| 090 | rv_BB_dxCas9_2 | BioBrick | CTGCAGCGGCCGCTACTAGTAttatGGCCGGCCcaccttcctcttcttcttg |
| 091 | fw_big_bovine_alb | Sample Preparation | CCCCCGTATCTGTCATTTCTTT |
| 092 | rv_big_bovine_alb | Sample Preparation | GGCATGGCAACCCACTGC |
| 093 | fw_dxcas_BL_bb | BioBrick | P-TACTAGTAGCGGCCGCTGCAGTCCGG |
| 094 | rv_dxcas_BL_bb | BioBrick | P-ttacaccttcctcttcttcttggggtcagccct |
| 095 | EPO-2 | d-AuNPs | TCCTGTCCCTGCTGTCGCTC |
| 096 | EPO-3 | d-AuNPs | GCCTGGAAGAGGATGGAGGT |
| 097 | EPO-4 | d-AuNPs | CTGCATGTGGATAAAGCCGT |
| 098 | EPO-5 | d-AuNPs | CGAACAATCACTGCTGACAC |
| 099 | fw_BB_sgRNA_EPO1 | BioBrick | gaattcgcggccgcttctagagGGAGGCCGAGAATATCAC |
| 100 | fw_BB_sgRNA_EPO2 | BioBrick | gaattcgcggccgcttctagagACAGCCCGTCGTGATATTCT |
| 101 | fw_BB_T7_sgRNA | BioBrick | gaattcgcggccgcttctagagaagcTAATACGACTCACTATAGG |
| 102 | rv_BB_sgRNA | BioBrick | ctgcagcggccgctactagtaGCACCGACTCGGTGCCAC |
| 103 | SeqTim_01 | Sequencing | GATATGAATAAATTGCAGTTTGTCGGGCAGCAGGCCGTAGA |
| 104 | SeqTim_02 | Sequencing | CATCATTGGCAACGCTACCCTCCATCCTCTTCCAGGCAT |
| 105 | SeqTim_03 | Sequencing | TCTACGGCCTGCTGCCCGACAAACTGCAATTTATTCATATC |
| 106 | SeqTim_04 | Sequencing | ATGCCTGGAAGAGGATGGAGGGTAGCGTTGCCAATGATG |
| 107 | SeqTim_05 | Sequencing | TTCGGGCTCTGGGAGCCCAGccgcctccatccagtctatt |
| 108 | SeqTim_06 | Sequencing | GGAGGGGAGATGGCTTCCTTgaatgaagccataccaaacg |
| 109 | SeqTim_07 | Sequencing | cgtttggtatggcttcattcAAGGAAGCCATCTCCCCTCC |
| 110 | SeqTim_08 | Sequencing | aatagactggatggaggcggCTGGGCTCCCAGAGCCCGAA |
| ID | Name | Module | Sequence (5' to 3') |
|---|---|---|---|
| 001 | fw_IV1 | In Vivo | CCCTGAACCGACGACCGG |
| 002 | rv_IV1 | In Vivo | GCAGTGTGACCGTGTGCTTC |
| 003 | fw_IV2 | In Vivo | CTGAAACCTCAGGCATTTGAGAAGC |
| 004 | rv_IV2 | In Vivo | GAAATTCGAAAGCAAATTCGACCCGG |
| 005 | fw_KanR | In Vivo | CTAGCTGTCTCTTATACACATCTTTAGAAAAACTCATCGAGCATCAAATG |
| 006 | rv_KanR | In Vivo | GACTCTGTCTCTTATACACATCTATGAGCCATATTCAACGGG |
| 007 | fw_diagnostic | In Vivo | CCGGTAATAATCCACAGCAG |
| 008 | rv_diagnostic | In Vivo | CCAGATTCAGACAGCCAATC |
| 009 | fw_KanRCassetteME | In Vivo | CTAGCTGTCTCTTATACACATCTTAAcTAAcTAAcagctcgagtcccgtcaag |
| 010 | rv_KanRCassetteME | In Vivo | CTAGCTGTCTCTTATACACATCTTAAcTAAcTAAcaaagccacgttgtgtctc |
| 011 | fw_KanRCassette | In Vivo | TAAcTAAcTAAcagctcgagtcccgtc |
| 012 | rv_KanRCassette | In Vivo | TAAcTAAcTAAcaaagccacgttgtgtctc |
| 013 | fw_KanRCassetteME | In Vivo | cttggaCTGTCTCTTATACACATCTgacagctcgagtcccgtc |
| 014 | rv_KanRCassetteME | In Vivo | tgtcatCTGTCTCTTATACACATCTattcaacaaagccacgttgtgtctc |
| 015 | fw_KanRCassette | In Vivo | ctgacagctcgagtcccgtc |
| 016 | rv_KanRCassette | In Vivo | attcaacaaagccacgttgtgtctc |
| 017 | fw_KanRMEBiobrick | BioBrick | gaattcgcggccgcttctagagcttggaCTGTCTCTTATACACATCTgacag |
| 018 | rv_KanRMEBiobrick | BioBrick | tactagtagcggccgctgcagtgtcatCTGTCTCTTATACACATCTattcaacaaagc |
| 019 | rv_KanR internal | BioBrick | gcagtttcatttgatgctcgatgag |
| 020 | fw_KanR internal | BioBrick | ggctcataacaccccttgtattactg |
| 021 | fw target seq LacZ | In Vivo | TCGCACAGCGTGTACCACAG |
| 022 | rv target seq LacZ | In Vivo | CTGTGGTACACGCTGTGCG |
| 023 | fw_pSB1C3ori | BioBrick | GCAAGCAGCAGATTACGCG |