Difference between revisions of "Team:TUDelft/Primers/Main"

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   </tr>
 
   </tr>
 
   <tr class="tableunevengrnbl">
 
   <tr class="tableunevengrnbl">
−
     <td>001</td>
+
     <td>IV001</td>
 
     <td>fw_IV1</td>
 
     <td>fw_IV1</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
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   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
−
     <td>002</td>
+
     <td>IV002</td>
 
     <td>rv_IV1</td>
 
     <td>rv_IV1</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
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   </tr>
 
   </tr>
 
   <tr class="tableunevengrnbl">
 
   <tr class="tableunevengrnbl">
−
     <td>003</td>
+
     <td>IV003</td>
 
     <td>fw_IV2</td>
 
     <td>fw_IV2</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
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   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
−
     <td>004</td>
+
     <td>IV004</td>
 
     <td>rv_IV2</td>
 
     <td>rv_IV2</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
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   </tr>
 
   </tr>
 
   <tr class="tableunevengrnbl">
 
   <tr class="tableunevengrnbl">
−
     <td>005</td>
+
     <td>IV005</td>
 
     <td>fw_KanR</td>
 
     <td>fw_KanR</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
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   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
−
     <td>006</td>
+
     <td>IV006</td>
 
     <td>rv_KanR</td>
 
     <td>rv_KanR</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
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   </tr>
 
   </tr>
 
   <tr class="tableunevengrnbl">
 
   <tr class="tableunevengrnbl">
−
     <td>007</td>
+
     <td>IV007</td>
 
     <td>fw_diagnostic</td>
 
     <td>fw_diagnostic</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
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   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
−
     <td>008</td>
+
     <td>IV008</td>
 
     <td>rv_diagnostic</td>
 
     <td>rv_diagnostic</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
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   </tr>
 
   </tr>
 
   <tr class="tableunevengrnbl">
 
   <tr class="tableunevengrnbl">
−
     <td>009</td>
+
     <td>IV009</td>
 
     <td>fw_KanRCassetteME</td>
 
     <td>fw_KanRCassetteME</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
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   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
−
     <td>010</td>
+
     <td>IV010</td>
 
     <td>rv_KanRCassetteME</td>
 
     <td>rv_KanRCassetteME</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
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   </tr>
 
   </tr>
 
   <tr class="tableunevengrnbl">
 
   <tr class="tableunevengrnbl">
−
     <td>011</td>
+
     <td>IV011</td>
 
     <td>fw_KanRCassette</td>
 
     <td>fw_KanRCassette</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
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   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
−
     <td>012</td>
+
     <td>IV012</td>
 
     <td>rv_KanRCassette</td>
 
     <td>rv_KanRCassette</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
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   </tr>
 
   </tr>
 
   <tr class="tableunevengrnbl">
 
   <tr class="tableunevengrnbl">
−
     <td>013</td>
+
     <td>IV013</td>
 
     <td>fw_KanRCassetteME</td>
 
     <td>fw_KanRCassetteME</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
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   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
−
     <td>014</td>
+
     <td>IV014</td>
 
     <td>rv_KanRCassetteME</td>
 
     <td>rv_KanRCassetteME</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
Line 796: Line 796:
 
   </tr>
 
   </tr>
 
   <tr class="tableunevengrnbl">
 
   <tr class="tableunevengrnbl">
−
     <td>015</td>
+
     <td>IV015</td>
 
     <td>fw_KanRCassette</td>
 
     <td>fw_KanRCassette</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
Line 802: Line 802:
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
−
     <td>016</td>
+
     <td>IV016</td>
 
     <td>rv_KanRCassette</td>
 
     <td>rv_KanRCassette</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
Line 808: Line 808:
 
   </tr>
 
   </tr>
 
   <tr class="tableunevengrnbl">
 
   <tr class="tableunevengrnbl">
−
     <td>017</td>
+
     <td>IV017</td>
 
     <td>fw_KanRMEBiobrick</td>
 
     <td>fw_KanRMEBiobrick</td>
 
     <td>BioBrick</td>
 
     <td>BioBrick</td>
Line 814: Line 814:
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
−
     <td>018</td>
+
     <td>IV018</td>
 
     <td>rv_KanRMEBiobrick</td>
 
     <td>rv_KanRMEBiobrick</td>
 
     <td>BioBrick</td>
 
     <td>BioBrick</td>
Line 820: Line 820:
 
   </tr>
 
   </tr>
 
   <tr class="tableunevengrnbl">
 
   <tr class="tableunevengrnbl">
−
     <td>019</td>
+
     <td>IV019</td>
 
     <td>rv_KanR internal</td>
 
     <td>rv_KanR internal</td>
 
     <td>BioBrick</td>
 
     <td>BioBrick</td>
Line 826: Line 826:
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
−
     <td>020</td>
+
     <td>IV020</td>
 
     <td>fw_KanR internal</td>
 
     <td>fw_KanR internal</td>
 
     <td>BioBrick</td>
 
     <td>BioBrick</td>
Line 832: Line 832:
 
   </tr>
 
   </tr>
 
   <tr class="tableunevengrnbl">
 
   <tr class="tableunevengrnbl">
−
     <td>021</td>
+
     <td>IV021</td>
 
     <td>fw target seq LacZ</td>
 
     <td>fw target seq LacZ</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
Line 838: Line 838:
 
   </tr>
 
   </tr>
 
   <tr class="tableevengrnbl">
 
   <tr class="tableevengrnbl">
−
     <td>022</td>
+
     <td>IV022</td>
 
     <td>rv target seq LacZ</td>
 
     <td>rv target seq LacZ</td>
 
     <td></em>In Vivo</em></td>
 
     <td></em>In Vivo</em></td>
Line 844: Line 844:
 
   </tr>
 
   </tr>
 
   <tr class="tableunevengrnbl">
 
   <tr class="tableunevengrnbl">
−
     <td>023</td>
+
     <td>IV023</td>
 
     <td>fw_pSB1C3ori</td>
 
     <td>fw_pSB1C3ori</td>
 
     <td>BioBrick</td>
 
     <td>BioBrick</td>

Revision as of 21:00, 13 October 2018

partssequences

Primers

ID Name Module Sequence (5' to 3')
001 fw_dxCas9 Fusion Protein atataGCGGCCGCtatggacaagaagtactccat
002 rv_dxCas9 Fusion Protein tatGGTACCttatGGCCGGCCcaccttcctcttcttcttgg
003 fw_dxCas9_mut_EcoRI Fusion Protein P-gcgaattttccaaaagagtgatcc
004 rv_dxCas9_mut_EcoRI Fusion Protein P-ttatttgctcgatgatctcatcaagg
005 fw_Tn5_afterC Fusion Protein tataGGCCGGCCtAAACTGGGCGGCGGCGCGCCGGCGGTGGGCGGCGGCCCGAAAGCGGCGGATAAAatgattaccagtgcactgcatc
006 rv_Tn5_afterC Fusion Protein tataGGTACCttagattttaatgccctgcgcca
007 fw_Tn5_beforeC Fusion Protein tataGAATTCtatgattaccagtgcactgcatc
008 rv_Tn5_beforeC Fusion Protein tataGCGGCCGCTTTATCCGCCGCTTTCGGGCCGCCGCCCACCGCCGGCGCGCCGCCGCCCAGTTTgattttaatgccctgcgcca
009 rv_dxCas9_mut1_GA Fusion Protein cattactaaagatctcttgcaggtagca
010 fw_dxCas9_mut1_GA Fusion Protein gctacctgcaagagatctttagtaatg
011 rv_dxCas9_mut2_GA Fusion Protein gaccttaacggtttgcagtattcc
012 fw_dxCas9_mut2_GA Fusion Protein ggaatactgcaaaccgttaagg
013 rv_dxCas9_mut3_GA Fusion Protein ccgttttgcaggtagtacag
014 fw_dxCas9_mut3_GA Fusion Protein ctgtactacctgcaaaacgg
015 rv_dxCas9_mut4_GA Fusion Protein tcgttacctttttgcagcac
016 fw_dxCas9_mut4_GA Fusion Protein tgctgcaaaaaggtaacgag
017 rv_dxCas9_mut5_GA Fusion Protein tacttgaaggctgccggc
018 fw_dxCas9_mut_GA Fusion Protein gccggcagccttcaag
019 fw_dxCas9_mut1_BL Fusion Protein P-gctacctgcaagagatctttagtaatg
020 rv_dxCas9_mut1_BL Fusion Protein P-agatccgattctttctgcgg
021 fw_dxCas9_mut2_BL Fusion Protein P-ggaatactgcaaaccgttaagg
022 rv_dxCas9_mut2_BL Fusion Protein P-ctttttgatagctgggctacct
023 fw_dxCas9_mut3_BL Fusion Protein P-ctgtactacctgcaaaacgg
024 rv_dxCas9_mut3_BL Fusion Protein P-gtagagcttctcattctgaagc
025 fw_dxCas9_mut4_BL Fusion Protein P-ctgcaaaaaggtaacgagctg
026 rv_dxCas9_mut4_BL Fusion Protein P-cacgcccgcactagc
027 fw_dxCas9_mut5_BL Fusion Protein P-gccggcagccttcaag
028 rv_dxCas9_mut5_BL Fusion Protein P-gcgcccaagttggtca
029 fw_T7_promoter General TAATACGACTCACTATAGGG
030 rv_T7_promoter General GCTAGTTATTGCTCAGCGG
031 VF2 General tgccacctgacgtctaagaa
032 VR General attaccgcctttgagtgagc
033 fw_sgRNA_1 Fusion Protein aagcTAATACGACTCACTATAGGGGAGGCCGAGAATATCA
034 rv_sgRNA_1 Fusion Protein AAAAGCACCGACTCGGTGCCACTT
035 fw_sgRNA_2 Fusion Protein aagcTAATACGACTCACTATAGGACAGCCCGTCGTGATATTCTGTTTTAGAGCTAGAAATAG
036 fw_adTn5_1 Fusion Protein CTAGCTGTCTCTTATACACATCTATTGGCGAAATGGATGAACTGTTTACCATGC
037 rv_adTn5_1 Fusion Protein GCATGGTAAACAGTTCATCCATTTCGCCAATAGATGTGTATAAGAGACAGCTAG
038 fw_1_AUpart d-AuNPs GATCGAATTCGCGGCCGCTTC
039 rv_1_AUpart d-AuNPs CGATTCTGCAGCGGCCGCTAC
040 fw_2_AUpart d-AuNPs GGAGGCCGAGAATATCACGA
041 rv_2_AUpart d-AuNPs TTGAGATCGTTTTGGTCTGC
042 fw_3_AUpart d-AuNPs CAGGTCGACAAGCTTGCG
043 fw_dCas9 Fusion Protein atataGCGGCCGCtatggataagaaatactcaataggcttagc
044 rv_dCas9 Fusion Protein tatGGTACCttatGGCCGGCCgtcacctcctagctgactc
045 fw_1_Tn5-L-dxCas9_Gblock Fusion Protein ACCATGGGCAGCAGC
046 rv_1_Tn5-L-dxCas9_Gblock Fusion Protein GTAGGGTATCCGAAATGTGAGGATTTTC
047 fw_2_Tn5-L-dxCas9_Gblock Fusion Protein GGGAAAAGATTGAGAAAATCCTCACATTTCG
048 rv_2_Tn5-L-dxCas9_Gblock Fusion Protein TGGCGTTCAAATTTCGCAGC
049 fw_pACYC-Duet_Backbone Fusion Protein GGTACCCTCGAGTCTGG
050 rv_pACYC-Duet_Backbone Fusion Protein GAATTCGGATCCTGGCTG
051 fw_BB_pSB1C3 BioBrick TACTAGTAGCGGCCGCTGC
052 rv_BB_pSB1C3 BioBrick CTAGAAGCGGCCGCGA
053 rv_BB_pSB1C3_extraNuc BioBrick CTCTAGAAGCGGCCGCGA
054 fw_BB_Tn5 BioBrick GCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGatgattaccagtgcactgcatcg
055 rv_BB_Tn5 BioBrick CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAttagattttaatgccctgcgccatc
056 fw_BB_dxCas9 BioBrick GCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGatggacaagaagtactccattgg
057 rv_BB_dxCas9 BioBrick CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAttatggccggcccac
058 fw_BB_Linker BioBrick CGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGCCGGCCtAAACTGG
059 rv_BB_Linker BioBrick CTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTTATCCGCCGCTTTCGGGCCGCCGC
060 fw_BB_T7p BioBrick ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGGAATTGTG
061 rv_BB_T7p BioBrick TTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAGCTAGTTATTGCTCAGCGGTG
062 fw_adTn5_2 Fusion Protein CTGTCTCTTATACACATCTATTGGCGAAATGGATGAACTGTTTACCATGC
063 rv_adTn5_2 Fusion Protein GCATGGTAAACAGTTCATCCATTTCGCCAATAGATGTGTATAAGAGACAG
064 rv_adTn5_label Fusion Protein Cy-GCATGGTAAACAGTTCATCCATTTCGCCAATAGATGTGTATAAGAGACAG
065 fw_1_AUpart d-AuNPs Cy-GATCGAATTCGCGGCCGCTTC
066 fw_bovine_albumin Sample Preparation TTGTTTCAACTCAAACAGCCTT
067 rv_bovine_albumin Sample Preparation AGAATCCCCATGGACAGAGA
068 fw_vectorxcas_spike Sample Preparation AGTAGCGGCCGCTGCAGaaggaagaggctgcaatctgtggc
069 rv_vectorxcas_spike Sample Preparation AGAAGCGGCCGCGAATTCcacacaagcagggagcagatactgg
070 fw_epo_spike Sample Preparation tgctccctgcttgtgtgGAATTCGCGGCCGCTTCT
071 rv_epo_spike Sample Preparation gattgcagcctcttccttCTGCAGCGGCCGCTAC
072 fw_epo_frag Sample Preparation GCCTCCCAGTCCTGGGCG
073 rv_epo_frag Sample Preparation GCAGTTGCAGGGGCTCCCAC
074 fw_epo_check Sample Preparation CCTGGAGAGGTACCTCTTGGAGGC
075 rv_epo_check Sample Preparation GCCCGACCTCCATCCTCTTCCAG
076 rv_adKan150 Fusion Protein catcctatggaactgcctcggtgagttttctccttc
077 rv_adKan300 Fusion Protein gaaatgcataagcttttgccattctcaccggattc
078 rv_adKan800 Fusion Protein gagccatattcaacgggaaacgtcttgctcc
079 fw_3_dxCas9-L-Tn5_Gblock Fusion Protein agaaataattttgtttaactttaataaggagatataccatgggc
080 rv_3_dxCas9-L-Tn5_Gblock Fusion Protein CTCGAATCAGTTTGTCATTTTCATCGTACTTG
081 fw_4_dxCas9-L-Tn5_Gblock Fusion Protein CCCAAATTCTCGATTCACGCATG
082 rv_4_dxCas9-L-Tn5_Gblock Fusion Protein tttagaggccccaaggggttatg
083 fw_2pACYC-Duet_Backbone Fusion Protein CTGCTGCCACCGCTG
084 rv_2pACYC-Duet_Backbone Fusion Protein GCTGCTGCCCATGGTATATC
085 fw_gblock1_mut_EcoRI Fusion Protein CAGGATCCGAATACTATGATTACC
086 rv_gblock1_mut_EcoRI Fusion Protein GGTAATCATAGTATTCGGATCCTG
087 fw_adTn5_Phos Fusion Protein P-CTGTCTCTTATACACATCT
088 fw_1_Tn5-dxCas9_Gblock_option2 Fusion Protein accatgggcagcagccatcaccatcatcaccacagcc
089 rv_1_Tn5-dxCas9_Gblock_option2 Fusion Protein cagggaaaagattgagaaaatcctcacatttcggataccctac
090 rv_BB_dxCas9_2 BioBrick CTGCAGCGGCCGCTACTAGTAttatGGCCGGCCcaccttcctcttcttcttg
091 fw_big_bovine_alb Sample Preparation CCCCCGTATCTGTCATTTCTTT
092 rv_big_bovine_alb Sample Preparation GGCATGGCAACCCACTGC
093 fw_dxcas_BL_bb BioBrick P-TACTAGTAGCGGCCGCTGCAGTCCGG
094 rv_dxcas_BL_bb BioBrick P-ttacaccttcctcttcttcttggggtcagccct
095 EPO-2 d-AuNPs TCCTGTCCCTGCTGTCGCTC
096 EPO-3 d-AuNPs GCCTGGAAGAGGATGGAGGT
097 EPO-4 d-AuNPs CTGCATGTGGATAAAGCCGT
098 EPO-5 d-AuNPs CGAACAATCACTGCTGACAC
099 fw_BB_sgRNA_EPO1 BioBrick gaattcgcggccgcttctagagGGAGGCCGAGAATATCAC
100 fw_BB_sgRNA_EPO2 BioBrick gaattcgcggccgcttctagagACAGCCCGTCGTGATATTCT
101 fw_BB_T7_sgRNA BioBrick gaattcgcggccgcttctagagaagcTAATACGACTCACTATAGG
102 rv_BB_sgRNA BioBrick ctgcagcggccgctactagtaGCACCGACTCGGTGCCAC
103 SeqTim_01 Sequencing GATATGAATAAATTGCAGTTTGTCGGGCAGCAGGCCGTAGA
104 SeqTim_02 Sequencing CATCATTGGCAACGCTACCCTCCATCCTCTTCCAGGCAT
105 SeqTim_03 Sequencing TCTACGGCCTGCTGCCCGACAAACTGCAATTTATTCATATC
106 SeqTim_04 Sequencing ATGCCTGGAAGAGGATGGAGGGTAGCGTTGCCAATGATG
107 SeqTim_05 Sequencing TTCGGGCTCTGGGAGCCCAGccgcctccatccagtctatt
108 SeqTim_06 Sequencing GGAGGGGAGATGGCTTCCTTgaatgaagccataccaaacg
109 SeqTim_07 Sequencing cgtttggtatggcttcattcAAGGAAGCCATCTCCCCTCC
110 SeqTim_08 Sequencing aatagactggatggaggcggCTGGGCTCCCAGAGCCCGAA
ID Name Module Sequence (5' to 3')
IV001 fw_IV1 In Vivo CCCTGAACCGACGACCGG
IV002 rv_IV1 In Vivo GCAGTGTGACCGTGTGCTTC
IV003 fw_IV2 In Vivo CTGAAACCTCAGGCATTTGAGAAGC
IV004 rv_IV2 In Vivo GAAATTCGAAAGCAAATTCGACCCGG
IV005 fw_KanR In Vivo CTAGCTGTCTCTTATACACATCTTTAGAAAAACTCATCGAGCATCAAATG
IV006 rv_KanR In Vivo GACTCTGTCTCTTATACACATCTATGAGCCATATTCAACGGG
IV007 fw_diagnostic In Vivo CCGGTAATAATCCACAGCAG
IV008 rv_diagnostic In Vivo CCAGATTCAGACAGCCAATC
IV009 fw_KanRCassetteME In Vivo CTAGCTGTCTCTTATACACATCTTAAcTAAcTAAcagctcgagtcccgtcaag
IV010 rv_KanRCassetteME In Vivo CTAGCTGTCTCTTATACACATCTTAAcTAAcTAAcaaagccacgttgtgtctc
IV011 fw_KanRCassette In Vivo TAAcTAAcTAAcagctcgagtcccgtc
IV012 rv_KanRCassette In Vivo TAAcTAAcTAAcaaagccacgttgtgtctc
IV013 fw_KanRCassetteME In Vivo cttggaCTGTCTCTTATACACATCTgacagctcgagtcccgtc
IV014 rv_KanRCassetteME In Vivo tgtcatCTGTCTCTTATACACATCTattcaacaaagccacgttgtgtctc
IV015 fw_KanRCassette In Vivo ctgacagctcgagtcccgtc
IV016 rv_KanRCassette In Vivo attcaacaaagccacgttgtgtctc
IV017 fw_KanRMEBiobrick BioBrick gaattcgcggccgcttctagagcttggaCTGTCTCTTATACACATCTgacag
IV018 rv_KanRMEBiobrick BioBrick tactagtagcggccgctgcagtgtcatCTGTCTCTTATACACATCTattcaacaaagc
IV019 rv_KanR internal BioBrick gcagtttcatttgatgctcgatgag
IV020 fw_KanR internal BioBrick ggctcataacaccccttgtattactg
IV021 fw target seq LacZ In Vivo TCGCACAGCGTGTACCACAG
IV022 rv target seq LacZ In Vivo CTGTGGTACACGCTGTGCG
IV023 fw_pSB1C3ori BioBrick GCAAGCAGCAGATTACGCG