Difference between revisions of "Team:HZAU-China/Collaborations"

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             z-index: 1;
 
             z-index: 1;
 
             background-color: #EEEEEE;
 
             background-color: #EEEEEE;
−
            opacity: 0.96;
 
−
            filter: alpha(opacity=96);
 
 
             box-shadow: 0 5px 15px #CCCCCC;
 
             box-shadow: 0 5px 15px #CCCCCC;
 
             display: none;
 
             display: none;
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         }
 
         }
  
−
         .daohang a:hover span{
+
         .daohang a:hover span {
−
             transform: rotateY(180deg);          
+
             transform: rotateY(180deg);
−
             -webkit-transform: rotateY(180deg);          
+
             -webkit-transform: rotateY(180deg);
−
             -moz-transform: rotateY(180deg);          
+
             -moz-transform: rotateY(180deg);
−
             -o-transform: rotateY(180deg);          
+
             -o-transform: rotateY(180deg);
 
             -ms-transform: rotateY(180deg);
 
             -ms-transform: rotateY(180deg);
−
             transition: all 0.5s ease-in-out;          
+
             transition: all 0.5s ease-in-out;
−
             -webkit-transition: all 0.5s ease-in-out;          
+
             -webkit-transition: all 0.5s ease-in-out;
−
             -moz-transition: all 0.5s ease-in-out;          
+
             -moz-transition: all 0.5s ease-in-out;
 
             -o-transition: all 0.5s ease-in-out;
 
             -o-transition: all 0.5s ease-in-out;
 
         }
 
         }
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             .daohangyi {
 
             .daohangyi {
−
                 font-size: 14px;
+
                 font-size: 12px;
 
             }
 
             }
 
         }
 
         }
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             .daohangyi img {
 
             .daohangyi img {
 
                 display: none;
 
                 display: none;
 +
            }
 +
 +
            .daohang .longName .item:before {
 +
                width: 150px;
 +
                left: 20px;
 +
            }
 +
 +
            .daohang .longName .item:hover:after {
 +
                width: 150px;
 +
                left: 20px;
 
             }
 
             }
 
         }
 
         }
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             width: 80%;
 
             width: 80%;
 
             /* height: auto; */
 
             /* height: auto; */
−
             margin: 20px 20px 0 0;
+
             margin: 20px 40px 0 0;
 
             /* right: 2%; */
 
             /* right: 2%; */
 
             /* top: 90px; */
 
             /* top: 90px; */
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         .cebian {
 
         .cebian {
−
             width: 15%;
+
             width: 180px;
 
             /* height: 80vh; */
 
             /* height: 80vh; */
 
             float: left;
 
             float: left;
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             /* border:2px solid black */
 
             /* border:2px solid black */
 
             /* background-color: #323643 */
 
             /* background-color: #323643 */
 +
        }
 +
 +
        .zhengwen p{
 +
            text-align: justify !important;
 +
            font-family:  'Times New Roman',Helvetica,'Open Sans',  Arial, sans-serif !important;
 +
            color: #3B3B3B;
 +
            font-size: 26px !important;
 +
            padding-right: 20px;
 
         }
 
         }
  
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         .h1 {
 
         .h1 {
−
             height: 100px;
+
             height: 80px;
−
             line-height: 100px;
+
             line-height: 80px;
 
             font-weight: bold;
 
             font-weight: bold;
 
             font-family: 'Product Sans', sans-serif;
 
             font-family: 'Product Sans', sans-serif;
−
             font-size: 50px;
+
             font-size: 40px;
−
             color: black;
+
             color: #3B3B3B;
−
             border-bottom: 3px solid black;
+
             border-bottom: 2px solid #676767;
−
             /* margin: 0 2.4%; */
+
            margin-bottom: 20px;
 +
        }
 +
 
 +
        .h2 {
 +
            height: 40px;
 +
            line-height: 40px;
 +
            font-weight: bold;
 +
            /* font-weight: bold; */
 +
            font-family: 'Product Sans', sans-serif;
 +
            font-size: 30px;
 +
            color: #484848;
 +
             /* margin-bottom: 20px; */
 +
        }
 +
 
 +
        .h3 {
 +
            height: 30px;
 +
            line-height: 30px;
 +
            font-weight: bold;
 +
            /* font-weight: bold; */
 +
            font-family: 'Product Sans', sans-serif;
 +
            font-size: 24px;
 +
            color: #484848;
 +
            /* margin-bottom: 20px; */
 
         }
 
         }
  
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         div.floatCtro {
 
         div.floatCtro {
 
             width: 100%;
 
             width: 100%;
−
             height: 250px;
+
             height: 60px;
 
             margin: 0;
 
             margin: 0;
 
             position: relative;
 
             position: relative;
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             line-height: 45px;
 
             line-height: 45px;
 
             font-family: Arial;
 
             font-family: Arial;
−
             font-size: 14px;
+
             font-size: 16px !important;
 
             color: #676767;
 
             color: #676767;
 
             margin: 0;
 
             margin: 0;
Line 616: Line 654:
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Experiments">Experiments</a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Experiments">Experiments</a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Improve">Improve</a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Improve">Improve</a>
−
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/InterLab">Interlab</a>
+
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/InterLab">InterLab</a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Notebook">Notebook</a>
 
             </li>
 
             </li>
 
             <li class="hiLight shortName">
 
             <li class="hiLight shortName">
Line 631: Line 670:
 
                     <span class="xjtPic"></span>
 
                     <span class="xjtPic"></span>
 
                 </a>
 
                 </a>
−
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Safety">Safty</a>
+
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Safety">Safety</a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Human_Practices">Human Practices</a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Human_Practices">Human Practices</a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Public_Engagement">Public Engagement</a>
 
                 <a class="item" href="https://2018.igem.org/Team:HZAU-China/Public_Engagement">Public Engagement</a>
Line 656: Line 695:
 
         <!-- 正文 -->
 
         <!-- 正文 -->
 
         <div class="zhengwen">
 
         <div class="zhengwen">
−
            <img class="daimg" src="https://static.igem.org/mediawiki/2018/7/70/T--HZAU-China--hezhao1.png" alt="">
 
 
             <div id="float01" class="cur">
 
             <div id="float01" class="cur">
−
                 <div class="h1">biaotiyi</div>
+
                 <div class="h1">Collaboration with WHU-China and SDU-CHINA</div>
 +
                <p>Optogenetic tools have broad prospects on synthetic biology, and the CcaS-CcaR system, which is
 +
                    developed from cyanobacteria has made obvious achievements, thus more and more teams are willing to
 +
                    use this system in their own projects. In the past two years, we have spent a great deal of time
 +
                    using it in our projects and accumulated a lot of experience(see more information in our previous
 +
                    wikis ). This year, we collaborated with WHU-China and SDU-CHINA who use this system in project on
 +
                    sharing the experience how we regulate the expression of CcaS and CcaR protein to control this
 +
                    optogenetic tools and what we achieved
 +
                </p>
  
 
             </div>
 
             </div>
 
             <div id="float02">
 
             <div id="float02">
−
                 <div class="h1">biaotier</div>
+
                 <div class="h1">Experience sharing with Worldshaper</div>
 +
                <p>As a dynamic high school team, Worldshaper demonstrates great enthusiasm in this competition. They
 +
                    visited our laboratory and we shared them our experience of iGEM competition in the past years with
 +
                    relaxed discussion, including preparations before competition, some experimental techniques, notes
 +
                    of standardization and guiding them to do multiple cell lines
 +
                </p>
  
 
             </div>
 
             </div>
 
             <div id="float03">
 
             <div id="float03">
−
                 <div class="h1">biaotisan</div>
+
                 <div class="h1">Help from NAU-CHINA</div>
−
                  
+
                 <p>After the information we got from meetings and public investigation, we thought it’s necessary to
 +
                    enhance the targeting of engineered salmonella we used, we designed OmpA-RGD protein to achieve
 +
                    this goal. RGD is a well-studied tumor homing tripeptide that specifically binds to alpha v beta 3
 +
                    (αvβ3) integrin, which is widely overexpressed on cancer cells. So there’s a cell line called
 +
                    MDA-MB-231 surface displaying αvβ3 needed for our further targeting and safety experiments, with
 +
                    the great help of NAU-CHINA , we had channels to get this cell line from Shanghai Institute of
 +
                    Biochemistry and Cell Biology.
 +
                </p>
 +
 
 
             </div>
 
             </div>
 
         </div>
 
         </div>
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             </a>
 
             </a>
 
             <div class="daohangyi">
 
             <div class="daohangyi">
−
                    <span class="biaoti">Collaborations</span>
+
                <span class="biaoti">Collaborations</span>
−
                    <span class="xsjPic"><img src="https://static.igem.org/mediawiki/2018/8/8d/T--HZAU-China--xjt.svg" alt=""></span>
+
                <span class="xsjPic"><img src="https://static.igem.org/mediawiki/2018/8/8d/T--HZAU-China--xjt.svg" alt=""></span>
−
                </div>
+
            </div>
 
             <div class="floatCtro">
 
             <div class="floatCtro">
−
                 <p class="daohanger">neirongyi</p>
+
                 <!-- <p class="daohanger">Project Support</p>
−
                 <p class="daohanger">neironger</p>
+
                 <p class="daohanger">Experience Sharing</p>
−
                 <p class="daohanger">neirongsan</p>
+
                 <p class="daohanger">neirongsan</p> -->
 
                 <a>
 
                 <a>
 
                     <span>Back&nbsp;to&nbsp;Top</span>
 
                     <span>Back&nbsp;to&nbsp;Top</span>

Revision as of 22:10, 13 October 2018

Collaboration with WHU-China and SDU-CHINA

Optogenetic tools have broad prospects on synthetic biology, and the CcaS-CcaR system, which is developed from cyanobacteria has made obvious achievements, thus more and more teams are willing to use this system in their own projects. In the past two years, we have spent a great deal of time using it in our projects and accumulated a lot of experience(see more information in our previous wikis ). This year, we collaborated with WHU-China and SDU-CHINA who use this system in project on sharing the experience how we regulate the expression of CcaS and CcaR protein to control this optogenetic tools and what we achieved

Experience sharing with Worldshaper

As a dynamic high school team, Worldshaper demonstrates great enthusiasm in this competition. They visited our laboratory and we shared them our experience of iGEM competition in the past years with relaxed discussion, including preparations before competition, some experimental techniques, notes of standardization and guiding them to do multiple cell lines

Help from NAU-CHINA

After the information we got from meetings and public investigation, we thought it’s necessary to enhance the targeting of engineered salmonella we used, we designed OmpA-RGD protein to achieve this goal. RGD is a well-studied tumor homing tripeptide that specifically binds to alpha v beta 3 (αvβ3) integrin, which is widely overexpressed on cancer cells. So there’s a cell line called MDA-MB-231 surface displaying αvβ3 needed for our further targeting and safety experiments, with the great help of NAU-CHINA , we had channels to get this cell line from Shanghai Institute of Biochemistry and Cell Biology.

Team Attributions
Collaborations
Back to Top