Difference between revisions of "Team:CUNY Kingsborough/Light Operon"

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<h1>Light Operon</h1>
 
<h1>Light Operon</h1>
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<h2>Introduction</h2>
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<p style="color:red;">Red=Dark-Dark state </p> 
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<p style="color:blue;">Blue=Dark-Light state </p>
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<p style="color:green;">Green=Light-Light state</p>  
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<h2>Modeling Results</h2>
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<p class="default-padding italicize">The different states of Yf1 are being hit with different intensities of light, producing alternating results unique to the state.</p>
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<ol>
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<h3><li>Starting concentration of 95 FixJ molecules (Gillespie)</li></h3>
 
<figure>
 
<figure>
 
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       <a href="https://static.igem.org/mediawiki/2018/4/41/T--CUNY_Kingsborough--4219light95.png" target="_blank"><img src="https://static.igem.org/mediawiki/2018/4/41/T--CUNY_Kingsborough--4219light95.png" style="width:80%"></a>
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       <a href="https://static.igem.org/mediawiki/2018/4/41/T--CUNY_Kingsborough--4219light95.png" target="_blank"><img src="https://static.igem.org/mediawiki/2018/4/41/T--CUNY_Kingsborough--4219light95.png" style="width:70%"></a>
 
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       <a href="https://static.igem.org/mediawiki/2018/e/ed/T--CUNY_Kingsborough--04219light.png" target="_blank"><img src="https://static.igem.org/mediawiki/2018/e/ed/T--CUNY_Kingsborough--04219light.png" style="width:80%"></a>
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       <a href="https://static.igem.org/mediawiki/2018/e/ed/T--CUNY_Kingsborough--04219light.png" target="_blank"><img src="https://static.igem.org/mediawiki/2018/e/ed/T--CUNY_Kingsborough--04219light.png" style="width:70%"></a>
 
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   <figcaption>
 
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     <center><span>(Left) Constant light at 0.4291 micromoles per square meter; (Right) Constant light at 4.291 micromoles per square meter</span></center>
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     <center><span>(Left) Constant light at 0.4291 micromoles per square meter; (Right) Constant light at 4.291 micromoles per square meter<br></span>
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    <span style="color:red;">yDD (Dark-Dark state); </span>
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    <span style="color:blue;">yDL/LD (Dark-Light state); </span>
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    <span style="color:green;">yLL (Light-Light state)</span>
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    </center>
 
   </figcaption>
 
   </figcaption>
 
</figure>
 
</figure>
  
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<h3><li>Starting concentration of 948 FixJ molecules (Gillespie)</li></h3>
 
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       <a href="https://static.igem.org/mediawiki/2018/4/41/T--CUNY_Kingsborough--4219light95.png" target="_blank"><img src="https://static.igem.org/mediawiki/2018/4/41/T--CUNY_Kingsborough--4219light95.png" style="width:80%"></a>
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       <a href="https://static.igem.org/mediawiki/2018/4/41/T--CUNY_Kingsborough--4219light95.png" target="_blank"><img src="https://static.igem.org/mediawiki/2018/4/41/T--CUNY_Kingsborough--4219light95.png" style="width:70%"></a>
 
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       <a href="https://static.igem.org/mediawiki/2018/6/67/T--CUNY_Kingsborough--04219light948%281%29.png" target="_blank"><img src="https://static.igem.org/mediawiki/2018/6/67/T--CUNY_Kingsborough--04219light948%281%29.png" style="width:80%"></a>
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       <a href="https://static.igem.org/mediawiki/2018/6/67/T--CUNY_Kingsborough--04219light948%281%29.png" target="_blank"><img src="https://static.igem.org/mediawiki/2018/6/67/T--CUNY_Kingsborough--04219light948%281%29.png" style="width:70%"></a>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
  
 
   <figcaption>
 
   <figcaption>
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     <center><span>(Left) Constant light at 0.4291 micromoles per square meter; (Right) Constant light at 4.291 micromoles per square meter</span></center>
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     <center><span>(Left) Constant light at 0.4291 micromoles per square meter; (Right) Constant light at 4.291 micromoles per square meter<br></span>
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    <span style="color:red;">yDD (Dark-Dark state); </span>
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    <span style="color:blue;">yDL/LD (Dark-Light state); </span>
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    <span style="color:green;">yLL (Light-Light state)</span>
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    </center>
 
   </figcaption>
 
   </figcaption>
 
</figure>
 
</figure>
  
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<hr>
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<br><hr>
  
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<h3><li>Comparison to deterministic models</li></h3>
 
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   <figcaption>
 
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     <center>
 
     <center>
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    <span style>(Left) Starting concentration of 95 FixJ molecules; (Right) Starting concentration of 948 FixJ molecules<br></span>
 
     <span style="color:blue;">yDD (Dark-Dark state); </span>  
 
     <span style="color:blue;">yDD (Dark-Dark state); </span>  
 
     <span style="color:orange;">yDL/LD (Dark-Light state); </span>  
 
     <span style="color:orange;">yDL/LD (Dark-Light state); </span>  
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</figure>
 
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</ol>
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<p> The different states Yf1 are being hit with different intensities of light, producing alternating results unique to the state.</p><br>
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</body>
 
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Revision as of 23:01, 13 October 2018

Background Design Experiments Results
Overview Modeling 101 Light Operon Turing Patterns CRISPR-Cas13a
Human Practices Public Engagement
Team Members Collaborations Attributions

Light Operon

Introduction

Modeling Results

The different states of Yf1 are being hit with different intensities of light, producing alternating results unique to the state.

  1. Starting concentration of 95 FixJ molecules (Gillespie)

    2. (Left) Constant light at 0.4291 micromoles per square meter; (Right) Constant light at 4.291 micromoles per square meter
     yDD (Dark-Dark state); yDL/LD (Dark-Light state); yLL (Light-Light state)

  2. Starting concentration of 948 FixJ molecules (Gillespie)

    3. (Left) Constant light at 0.4291 micromoles per square meter; (Right) Constant light at 4.291 micromoles per square meter
     yDD (Dark-Dark state); yDL/LD (Dark-Light state); yLL (Light-Light state)

  3. Comparison to deterministic models

    4. (Left) Starting concentration of 95 FixJ molecules; (Right) Starting concentration of 948 FixJ molecules
     yDD (Dark-Dark state); yDL/LD (Dark-Light state); yLL (Light-Light state)