Difference between revisions of "Team:MIT/Safety"

(Prototype team page)
 
Line 1: Line 1:
−
{{MIT}}
 
 
<html>
 
<html>
 +
<head>
 +
<meta http-equiv="X-UA-Compatible" content="IE=Edge"/>
 +
    <meta name="viewport" content="width=device-width, initial-scale=1">   
 +
    <style type="text/css">
  
  
−
<div class="column full_size">
 
  
−
<h1> Safety </h1>
+
/*GOOGLE FONTS*/
−
<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
+
  
−
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
+
/* latin */
 +
@font-face {
 +
  font-family: 'Dosis';
 +
  font-style: normal;
 +
  font-weight: 400;
 +
  src: local('Dosis Regular'), local('Dosis-Regular'), url(https://static.igem.org/mediawiki/2018/4/4d/T--MIT--dosis2.woff) format('woff');
 +
  unicode-range: U+0000-00FF, U+0131, U+0152-0153, U+02BB-02BC, U+02C6, U+02DA, U+02DC, U+2000-206F, U+2074, U+20AC, U+2122, U+2191, U+2193, U+2212, U+2215, U+FEFF, U+FFFD;
 +
}
  
−
</div>
+
/* latin */
 +
@font-face {
 +
  font-family: 'Raleway';
 +
  font-style: normal;
 +
  font-weight: 400;
 +
  src: local('Raleway'), local('Raleway-Regular'),  url(https://static.igem.org/mediawiki/2018/5/59/T--MIT--raleway2.woff) format('woff');
 +
  unicode-range: U+0000-00FF, U+0131, U+0152-0153, U+02BB-02BC, U+02C6, U+02DA, U+02DC, U+2000-206F, U+2074, U+20AC, U+2122, U+2191, U+2193, U+2212, U+2215, U+FEFF, U+FFFD;
 +
}
  
 +
          #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, #sideMenu, #menubar, .logo_2017, .printfooter, .firstHeading,.visualClear {
 +
              display: none;
 +
          } /*-- hides default wiki settings --*/
  
−
<div class="column two_thirds_size">
+
          #top-section { /*-- styling for default menu bar (edit, page, history, etc.) --*/
−
<h3>Safe Project Design</h3>
+
              border: 0 none;
 +
              height: 3vw;
 +
              z-index: 100;
 +
              top: 0;
 +
              position: fixed;
 +
              width: 975px;
 +
              left: 50%;
 +
              margin-left: -487px;
 +
          }
  
−
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
+
          #top_title, #top {
 +
              display: none;
 +
              z-index: -1000;
 +
          }
  
−
<ul>
+
          .logo_2018 {
−
<li>Choosing a non-pathogenic chassis</li>
+
              display: none;
−
<li>Choosing parts that will not harm humans / animals / plants</li>
+
              z-index: -1000;
−
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
+
          }
−
<li>Including an "induced lethality" or "kill-switch" device</li>
+
−
</ul>
+
  
−
</div>
+
          #globalWrapper, #content { /*-- changes default wiki settings --*/
 +
              width: 100%;
 +
              height: 100%;
 +
              border: none;
 +
              background-color: black;
 +
              margin: 0px;
 +
              padding: 0px;
 +
              letter-spacing: normal;
 +
             
 +
              font-weight: light;
 +
          }
  
−
<div class="column third_size">
+
          html, body, .wrapper { /*-- changes default wiki settings --*/
−
<h3>Safe Lab Work</h3>
+
              width: 100%;
 +
              height: 100%;
 +
              background-color: black;
 +
          }
  
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+
.menu {
 +
        list-style-type: none;
 +
        margin: 0vw;
 +
        padding: .3vw;
 +
        /*overflow: hidden;*/
 +
        background-color: #333;
 +
        position: fixed;
 +
        width: 100%;
 +
        height: 4.5vw;
 +
        -webkit-box-shadow: 0 8px 6px -6px #2c2c2c;
 +
        -moz-box-shadow: 0 8px 6px -6px #2c2c2c;
 +
        box-shadow: 0 8px 6px -6px #2c2c2c;
 +
        top: 1.1vw;
 +
        z-index: 20;
 +
    }
  
 +
    .sub {
 +
        font-family: 'Dosis', sans-serif;;
 +
        font-size: 1.5vw;
 +
float: left;
 +
height: 4.5vw;
 +
       
 +
    }
  
−
<h3>Safe Shipment</h3>
+
    .sub a {
 +
        display: block;
 +
        color: white;
 +
        text-align: center;
 +
        padding: 1vw 1.5vw;
 +
        text-decoration: none;
 +
    }
  
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
+
    .sub a:hover {
−
</div>
+
        background-color: #410081;
 +
    }
  
 +
    .active {
 +
        background-color: #9055ff;
 +
    }
  
−
</html>
+
    .dropdown {
 +
        position: relative;
 +
        display: inline-block;
 +
    }
 +
 
 +
    .dropdown-content {
 +
        font-family: 'Raleway', sans-serif;;
 +
        font-size: 1.2vw;
 +
        display: none;
 +
        position: absolute;
 +
        top: 4vw;
 +
        background-color: #2c2c2c;
 +
        min-width: 10vw;
 +
        box-shadow: 0px 8px 16px 0px rgba(0,0,0,0.2);
 +
        z-index: 1;
 +
    }
 +
 
 +
    .dropdown-content a {
 +
        color: white;
 +
        padding: .6vw .9vw;
 +
        text-decoration: none;
 +
        display: block;
 +
    }
 +
 
 +
    .dropdown-content a:hover {background-color: #474747;}
 +
 
 +
    .dropdown:hover .dropdown-content {display: block;}
 +
 
 +
body{
 +
        margin:0;
 +
    }
 +
  .h{
 +
        color: #410081;
 +
        font-family: 'Dosis', sans-serif;;
 +
        font-size: 3.7vw;
 +
        position: absolute;
 +
line-height: 2.8vw;
 +
    }
 +
 
 +
    .p{
 +
        color: white;
 +
        font-family: 'Raleway', sans-serif;;
 +
        font-size: 1.8vw;
 +
line-height: 2.8vw;
 +
        z-index: 5;
 +
    }
 +
</style>
 +
</head>
 +
<body >
 +
 
 +
<img class="bg" src="https://static.igem.org/mediawiki/2018/f/f8/T--MIT--MITHomeBG.png" style="width:100%;">
 +
<div class="menu">
 +
        <div class="sub"><a class="active" href="#home">Home</a></div>
 +
        <div class="dropdown">
 +
            <div class="sub"><a href="https://2018.igem.org/Team:MIT/Team">Team</a></div>
 +
            <div class="dropdown-content">
 +
                <a href="https://2018.igem.org/Team:MIT/Team">Team Members</a>
 +
                <a href="#">Collaborations</a>
 +
            </div>
 +
        </div>
 +
        <div class="dropdown">
 +
            <div class="sub"><a href="https://2018.igem.org/Team:MIT/Description">Project</a></div>
 +
            <div class="dropdown-content">
 +
                <a href="#">Sensing</a>
 +
                <a href="#">Output</a>
 +
                <a href="#">Biofilm Assays</a>
 +
            </div>
 +
        </div>
 +
        <div class="dropdown">
 +
            <div class="sub"><a href="#project">Parts</a></div>
 +
            <div class="dropdown-content">
 +
                <a href="#">Basic Parts</a>
 +
                <a href="#">Composite Parts</a>
 +
            </div>
 +
        </div>
 +
        <div class="sub"><a href="#safety">Safety</a></div>
 +
        <div class="dropdown">
 +
            <div class="sub"><a href="#project">Human Practices</a></div>
 +
            <div class="dropdown-content">
 +
                <a href="https://2018.igem.org/Team:MIT/Human_Practices">Integrated Human Practices</a>
 +
                <a href="#">Public Engagement</a>
 +
            </div>
 +
        </div>
 +
        <div class="sub"><a href="#awards">Awards</a></div>
 +
    </div>
 +
 
 +
<div class="h"style="position: absolute; top: 2vw; left: 2vw;">
 +
Safety
 +
</div>
 +
 
 +
<div class="p" style="position: absolute; top: 4vw; left: 2vw;">
 +
In our project we are using HEK293 Cells which are a well documented line of cells that the team has been trained to handle properly. We are also using Streptococcus Mutans UA159, which is a strain of bacteria that is commonly found in the mouth. This strain of bacteria poses little to no threat to those that it could come in contact with unless they are immunocompromised. We also are not changing the S.Mutans’ DNA in any way to give them antibiotic resistance due to the chance that it could get out of the lab and cause harm to the environment.
 +
</div>
 +
<div class="p" style="position: absolute; top: 8vw; left: 2vw;">
 +
In our lab we consistently used proper PPE depending on what materials we were working with. Every team member was also required to go through lab specific trainings to be allowed to work with anything in the lab, and some of the team members were trained separately to deal with the s.mutans in the lab. While using the S.mutans we had a different set of procedures due to the bacteria’s vulnerability to contamination. This made it so that we used multiple sets of gloves during our experiments and also used a bunsen burner to decrease the likelihood of contamination.
 +
</div>

Revision as of 02:55, 14 October 2018

Home
Team
Team Members Collaborations
Project
Sensing Output Biofilm Assays
Parts
Basic Parts Composite Parts
Safety
Human Practices
Integrated Human Practices Public Engagement
Awards
Safety
In our project we are using HEK293 Cells which are a well documented line of cells that the team has been trained to handle properly. We are also using Streptococcus Mutans UA159, which is a strain of bacteria that is commonly found in the mouth. This strain of bacteria poses little to no threat to those that it could come in contact with unless they are immunocompromised. We also are not changing the S.Mutans’ DNA in any way to give them antibiotic resistance due to the chance that it could get out of the lab and cause harm to the environment.
In our lab we consistently used proper PPE depending on what materials we were working with. Every team member was also required to go through lab specific trainings to be allowed to work with anything in the lab, and some of the team members were trained separately to deal with the s.mutans in the lab. While using the S.mutans we had a different set of procedures due to the bacteria’s vulnerability to contamination. This made it so that we used multiple sets of gloves during our experiments and also used a bunsen burner to decrease the likelihood of contamination.