Difference between revisions of "Template:Virginia/Composite Part"

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<h1 id="description">Description</h1>
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<h1 id="composite-parts">Composite Parts</h1>
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<table style="width:100%" id="parts">
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    <tr>
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        <th>Name</th>
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        <th>Description</th>
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        <th>Part</th>
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    </tr>
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    <tr>
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        <td>LuxS-Block</td>
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        <td>(spacerLXf (K2535003) + I719005 + B0034 + K2535000 + B0015 + spacerLXr (K2535004)). LuxS is a protein, not native to the Lsr Operon, that catalyzes the cleavage of metabolites to synthesize the autoinducer molecule AI-2 (Gonzalez et al 2006). The LuxS gene is regulated by a T7 driven promoter in order to cooperate with the AI-2 sensitive plasmid that this part is used in conjunction with.
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        </td>
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        <td> spacer_pT7_rbs_LuxS_term_spacer -- BBa_K2535005 <em>SENT AS A BIOBRICK IN pSB1C3</em></td>
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    </tr>
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    <tr>
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        <td>LuxS 2nd Plasmid (LuxS-sfGFP)</td>
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        <td>I746909 + K2535005. This is a composite part of LuxS-Block and I746909. This part can be regulated using T7 to track LuxS expression based on the sfGFP reporter. Both LuxS and sfGFP are controlled by a T7 promoter. </td>
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        <td> pT7_rbs_sfGFP_term_LuxS-Block  -- K2535006</td>
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    </tr>
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    <tr>
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        <td>LsrK-Block</td>
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        <td>spacerLRf(K2535007) + I719005 + B0034 + K091002 + B0015 + spacerLRr (K2535008). LsrK is a kinase that is native to the Lsr Operon. It converts intracellular AI-2 to phosphorylated AI-2 (AI-2P), which derepresses the Lsr Operon (Xavier et al 2004). The LsrK gene is regulated by a T7 driven promoter in order to cooperate with the AI-2 sensitive plasmid that this part is used in conjunction with. </td>
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        <td> spacer_ pT7_rbs_LsrK_term_spacer -- K2535009</td>
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    </tr>
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    <tr>
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        <td>LsrK 2nd Plasmid (LsrK-sfGFP)</td>
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        <td>I746909 + K2535009. This is a composite part of LsrK-Block and I746909. This part can be regulated using T7 to track LsrK expression based on the sfGFP reporter. Both LsrK and sfGFP are controlled by a T7 promoter. </td>
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        <td>pT7_rbs_sfGFP_term_LsrK-Block -- K2535010</td>
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    </tr>
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    <tr>
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        <td>YdgG-Block</td>
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        <td>spacerYf(K2535011) + I719005 + B0034 + K2535002 + B0015 + spacerYr(K2535012).YdgG is a membrane transport protein, not native to the Lsr Operon, which exports AI-2 out of the cell (Herzberg et al 2006). The YdgG gene is regulated by a T7 driven promoter in order to cooperate with the AI-2 sensitive plasmid that this part is used in conjunction with. </td>
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        <td>spacer_ pT7_rbs_YdgG_term_spacer -- K2535013</td>
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    </tr>
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    <tr>
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        <td>YdgG 2nd Plasmid (YdgG-sfGFP)</td>
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        <td>I746909 + K2535013. This is a composite part of YdgG-Block and I746909. This part can be regulated using T7 to track YdgG expression based on the sfGFP reporter. Both YdgG and sfGFP are controlled by a T7 promoter. </td>
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        <td>pT7_rbs_sfGFP_term_YdgG-Block -- K2535014</td>
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    </tr>
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    <tr>
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        <td>LsrK-LuxS 2nd Plasmid (LsrK-LuxS-sfGFP)</td>
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        <td>I746909 + K2535009 + K2535005. This is a composite part of LsrK and LuxS put together with I746909 (sfGFP regulated by a T7 promoter). This part allows the upregulation of LuxS and LsrK so that intracellular AI-2 production and phosphorylation are increased concurrently.  </td>
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        <td> pt7_rbs_sfGFP_term_LsrK-Block_LuxS-Block -- K2535015</td>
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    </tr>
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    <tr>
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        <td>LsrK-LuxS-YdgG 2nd Plasmid (LsrK-LuxS-YdgG-sfGFP)</td>
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        <td>I746909 + K2535009 + K2535005 + K2535013. This is a composite part of LsrK, LuxS, and YdgG put together with I746909 (sfGFP regulated by a T7 promoter). This part allows the upregulation of LsrK, LuxS, and YdgG so that intracellular AI-2 production, AI-2 phosphorylation, and export out of the cell are all increased concurrently.  </td>
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        <td>  pt7_rbs_sfGFP_term_LsrK_LuxS_YdgG -- K2535017 <em>SENT AS A BIOBRICK IN pSB1C3</em> </td>
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    </tr>
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    <tr>
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        <td>sQS: Synthetic Quorum Response Plasmid Insert  (1st Plasmid)</td>
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        <td>B0014 (term) + K2535018 + K145001 (T7) + B0015 (term). Built in low copy pACYC cloning vector and optimized for use in a low copy vector to prevent leakiness of AI-2 regulated promoter. This plasmid contains an AI-2 regulated promoter, pLsr, which is bidirectional. Dimers of LsrR bind to and repress this promoter. However, AI-2P can bind to these dimers and cause them to dissociate, depressing the promoter.  Since the expression of this plasmid produces LsrR, which represses the promoter pLsr, this system is self regulating. sQS plasmid is used to regulate quorum enhancement plasmids. T7 is therefore only expressed in the presence of AI-2P. This plasmid can be used to regulate T7 expression based on AI-2 level in a media.
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        </td>
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        <td>term_pLsr_rbs_LsrR_rbs_T7_term -- K2535019</td>
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    </tr>
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</table>

Revision as of 05:30, 14 October 2018

Composite Parts

Name Description Part
LuxS-Block (spacerLXf (K2535003) + I719005 + B0034 + K2535000 + B0015 + spacerLXr (K2535004)). LuxS is a protein, not native to the Lsr Operon, that catalyzes the cleavage of metabolites to synthesize the autoinducer molecule AI-2 (Gonzalez et al 2006). The LuxS gene is regulated by a T7 driven promoter in order to cooperate with the AI-2 sensitive plasmid that this part is used in conjunction with. spacer_pT7_rbs_LuxS_term_spacer -- BBa_K2535005 SENT AS A BIOBRICK IN pSB1C3
LuxS 2nd Plasmid (LuxS-sfGFP) I746909 + K2535005. This is a composite part of LuxS-Block and I746909. This part can be regulated using T7 to track LuxS expression based on the sfGFP reporter. Both LuxS and sfGFP are controlled by a T7 promoter. pT7_rbs_sfGFP_term_LuxS-Block -- K2535006
LsrK-Block spacerLRf(K2535007) + I719005 + B0034 + K091002 + B0015 + spacerLRr (K2535008). LsrK is a kinase that is native to the Lsr Operon. It converts intracellular AI-2 to phosphorylated AI-2 (AI-2P), which derepresses the Lsr Operon (Xavier et al 2004). The LsrK gene is regulated by a T7 driven promoter in order to cooperate with the AI-2 sensitive plasmid that this part is used in conjunction with. spacer_ pT7_rbs_LsrK_term_spacer -- K2535009
LsrK 2nd Plasmid (LsrK-sfGFP) I746909 + K2535009. This is a composite part of LsrK-Block and I746909. This part can be regulated using T7 to track LsrK expression based on the sfGFP reporter. Both LsrK and sfGFP are controlled by a T7 promoter. pT7_rbs_sfGFP_term_LsrK-Block -- K2535010
YdgG-Block spacerYf(K2535011) + I719005 + B0034 + K2535002 + B0015 + spacerYr(K2535012).YdgG is a membrane transport protein, not native to the Lsr Operon, which exports AI-2 out of the cell (Herzberg et al 2006). The YdgG gene is regulated by a T7 driven promoter in order to cooperate with the AI-2 sensitive plasmid that this part is used in conjunction with. spacer_ pT7_rbs_YdgG_term_spacer -- K2535013
YdgG 2nd Plasmid (YdgG-sfGFP) I746909 + K2535013. This is a composite part of YdgG-Block and I746909. This part can be regulated using T7 to track YdgG expression based on the sfGFP reporter. Both YdgG and sfGFP are controlled by a T7 promoter. pT7_rbs_sfGFP_term_YdgG-Block -- K2535014
LsrK-LuxS 2nd Plasmid (LsrK-LuxS-sfGFP) I746909 + K2535009 + K2535005. This is a composite part of LsrK and LuxS put together with I746909 (sfGFP regulated by a T7 promoter). This part allows the upregulation of LuxS and LsrK so that intracellular AI-2 production and phosphorylation are increased concurrently. pt7_rbs_sfGFP_term_LsrK-Block_LuxS-Block -- K2535015
LsrK-LuxS-YdgG 2nd Plasmid (LsrK-LuxS-YdgG-sfGFP) I746909 + K2535009 + K2535005 + K2535013. This is a composite part of LsrK, LuxS, and YdgG put together with I746909 (sfGFP regulated by a T7 promoter). This part allows the upregulation of LsrK, LuxS, and YdgG so that intracellular AI-2 production, AI-2 phosphorylation, and export out of the cell are all increased concurrently. pt7_rbs_sfGFP_term_LsrK_LuxS_YdgG -- K2535017 SENT AS A BIOBRICK IN pSB1C3
sQS: Synthetic Quorum Response Plasmid Insert (1st Plasmid) B0014 (term) + K2535018 + K145001 (T7) + B0015 (term). Built in low copy pACYC cloning vector and optimized for use in a low copy vector to prevent leakiness of AI-2 regulated promoter. This plasmid contains an AI-2 regulated promoter, pLsr, which is bidirectional. Dimers of LsrR bind to and repress this promoter. However, AI-2P can bind to these dimers and cause them to dissociate, depressing the promoter. Since the expression of this plasmid produces LsrR, which represses the promoter pLsr, this system is self regulating. sQS plasmid is used to regulate quorum enhancement plasmids. T7 is therefore only expressed in the presence of AI-2P. This plasmid can be used to regulate T7 expression based on AI-2 level in a media. term_pLsr_rbs_LsrR_rbs_T7_term -- K2535019