Difference between revisions of "Team:Bio Without Borders/Notebook"
| Line 24: | Line 24: | ||
<dd>• gel electrophoresis for cPCR, nucleic acid purification plasmid </dd> | <dd>• gel electrophoresis for cPCR, nucleic acid purification plasmid </dd> | ||
<dt><b><font color="#009999">Week 3 (June 18-22)</font></b></dt> | <dt><b><font color="#009999">Week 3 (June 18-22)</font></b></dt> | ||
| − | <dd>•Made chloramphenicol plates</dd> | + | <dd>•Made chloramphenicol LB plates</dd> |
<dd>•Restriction enzyme digest of iGem plasmid using EcoRI and PstI</dd> | <dd>•Restriction enzyme digest of iGem plasmid using EcoRI and PstI</dd> | ||
| + | <dd>•Amplification of iGem backbones</dd> | ||
</dl> | </dl> | ||
Revision as of 15:09, 19 June 2018
Notebook
- Week 1 (June 4-8)
- •Made competent cells
- Week 2 (June 11-15)
- •Testing competent cells using iGem DNA transformation samples.
- • Tested to see if they grew better on a freezer block versus an ice bath.
- • Ran colony PCR and plated bacteria on antimicrobial plate.
- • cPCR, chloramphenicol, and culturing protocols, made agarose gel
- • gel electrophoresis for cPCR, nucleic acid purification plasmid
- Week 3 (June 18-22)
- •Made chloramphenicol LB plates
- •Restriction enzyme digest of iGem plasmid using EcoRI and PstI
- •Amplification of iGem backbones