Difference between revisions of "Team:Bio Without Borders/Notebook"
| Line 26: | Line 26: | ||
<dd>•Made chloramphenicol LB plates</dd> | <dd>•Made chloramphenicol LB plates</dd> | ||
<dd>•Restriction enzyme digest of iGem plasmid PSB1C3 using EcoRI and PstI</dd> | <dd>•Restriction enzyme digest of iGem plasmid PSB1C3 using EcoRI and PstI</dd> | ||
| − | <dd>•Made digest master mix; ran an ezyme digest of iGem plasmids PSB1A3 and PSB1K3</dd> | + | <dd>•Made digest master mix; ran an ezyme digest of iGem plasmids PSB1A3 and PSB1K3 |
| + | Performed ligation of </dd> | ||
| + | <dd>PSB1A3 and PSB1K3; transformed PSB1A3 in competent cells on ampicillin plate</dd> | ||
| + | |||
</dl> | </dl> | ||
Revision as of 19:19, 19 June 2018
Notebook
- Week 1 (June 4-8)
- •Made competent cells
- Week 2 (June 11-15)
- •Testing competent cells using iGem DNA transformation samples.
- • Tested to see if they grew better on a freezer block versus an ice bath.
- • Ran colony PCR and plated bacteria on antimicrobial plate.
- • cPCR, chloramphenicol, and culturing protocols, made agarose gel
- • gel electrophoresis for cPCR, nucleic acid purification plasmid
- Week 3 (June 18-22)
- •Made chloramphenicol LB plates
- •Restriction enzyme digest of iGem plasmid PSB1C3 using EcoRI and PstI
- •Made digest master mix; ran an ezyme digest of iGem plasmids PSB1A3 and PSB1K3 Performed ligation of
- PSB1A3 and PSB1K3; transformed PSB1A3 in competent cells on ampicillin plate