Difference between revisions of "Team:TUDelft/Parts/CompositeParts"

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When constructing our BioBricks, we made use of PCR amplification with our constructed expression plasmids as template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. Since our fusion protein <a href="http://parts.igem.org/Part:BBa_K2643000" class="grnbl" target="_blank">BBa_K2643000</a> consists of the dxCas9, linker and Tn5 coding sequences this part is registered as composite part. The <a href"https://2018.igem.org/Team:TUDelft/Basic_Part" class="grnbl">basic parts</a> comprising the our fusion protein composite part are documented separately and also exist as separate BioBricks. This is advantageous for those wishing to combine one of our basic parts with a different expression level or compose a different composite part altogether. The fusion BioBrick <a href="http://parts.igem.org/Part:BBa_K2643000" class="grnbl" target="_blank">BBa_K2643000</a>. is submitted with elaborate characterisation. Our favourite composite part is <a href="http://parts.igem.org/Part:BBa_K2643000" class="grnbl" target="_blank">BBa_K2643000</a>.
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<caption class="figgrnbl"><b>Table 1.</b> BioBrick Parts submitted by TU Delft iGEM 2018 team. All BioBricks were constructed by the team members involved in wetlab: Susan Bouwmeester, Kavish Kohabir, Venda Mangkusaputra, Timmy Paez, Lisbeth Schmidtchen and Nicole Bennis</caption>
  
 
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Revision as of 11:13, 14 October 2018

partsoverview

Composite Parts

When constructing our BioBricks, we made use of PCR amplification with our constructed expression plasmids as template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. Since our fusion protein BBa_K2643000 consists of the dxCas9, linker and Tn5 coding sequences this part is registered as composite part. The basic parts comprising the our fusion protein composite part are documented separately and also exist as separate BioBricks. This is advantageous for those wishing to combine one of our basic parts with a different expression level or compose a different composite part altogether. The fusion BioBrick BBa_K2643000. is submitted with elaborate characterisation. Our favourite composite part is BBa_K2643000.

Table 1. BioBrick Parts submitted by TU Delft iGEM 2018 team. All BioBricks were constructed by the team members involved in wetlab: Susan Bouwmeester, Kavish Kohabir, Venda Mangkusaputra, Timmy Paez, Lisbeth Schmidtchen and Nicole Bennis
Fave Part Name Type Description Designer
♥ BBa_K2643000 Composite dxCas9-linker-Tn5 fusion protein (HIS Tag) TU Delft 2018