When constructing our composite BioBrick, we made use of PCR amplification with our constructed expression plasmids as template to construct several BioBricks. Since our fusion protein <a href="http://parts.igem.org/Part:BBa_K2643000" class="grnbl" target="_blank">BBa_K2643000</a> consists of the dxCas9, linker and Tn5 coding sequences this part is registered as composite part. The <a href"" class="grnbl">basic parts</a> comprising the our fusion protein composite part are documented separately and also exist as separate BioBricks. This is advantageous for those wishing to combine one of our <a href="https://2018.igem.org/Team:TUDelft/Basic_Part" class="grnbl">basic parts</a> with a different expression level or compose a different composite part altogether. We created a <a href="https://2018.igem.org/Team:TUDelft/Part_Collection" class="grnbl">part collection</a> comprised of 6 basic parts encoding for different EPO coding sequences, 2 basic parts encoding for the required components for targeted sequencing and 1 composite part: our fusion protein. All BioBricks are submitted with elaborate characterisation so the user may choose the best protein for their project accordingly. Our favourite basic part is <a href="http://parts.igem.org/Part:BBa_K2643001" class="grnbl" target="_blank">BBa_K2643001</a> and our favourite composite part is <a href="http://parts.igem.org/Part:BBa_K2643000" class="grnbl" target="_blank">BBa_K2643000</a>.