Difference between revisions of "Team:ASIJ Tokyo/Experiments"

Line 98: Line 98:
 
     <li>d. <a href="https://static.igem.org/mediawiki/2018/2/2b/T--ASIJ_Tokyo--histag.pdf">Protein Purification using His Tag Protein Chromatography -  
 
     <li>d. <a href="https://static.igem.org/mediawiki/2018/2/2b/T--ASIJ_Tokyo--histag.pdf">Protein Purification using His Tag Protein Chromatography -  
 
         redone  Oct 10  (10 trials)</a></li>
 
         redone  Oct 10  (10 trials)</a></li>
−
     <li>c. <a href="https://static.igem.org/mediawiki/2018/8/84/T--ASIJ_Tokyo--esla.pdf">Sialic Acid Purification redone using new protocol (6 trials) </a></li>
+
     <li>e. <a href="https://static.igem.org/mediawiki/2018/8/84/T--ASIJ_Tokyo--esla.pdf">Sialic Acid Purification redone using new protocol (6 trials) </a></li>
−
     <li>d. Fluoroscence Microplate Reading to Quantify Protein Concentration of Constructs
+
     <li>f. Fluoroscence Microplate Reading to Quantify Protein Concentration of Constructs
 
         using GFP standard curve </li>
 
         using GFP standard curve </li>
−
     <li>e. Fluorescence Microplate Reading to Quantify Sialic Acid Concentration  
+
     <li>g. Fluorescence Microplate Reading to Quantify Sialic Acid Concentration  
 
         using Sialic Acid Standard Curve </li>
 
         using Sialic Acid Standard Curve </li>
−
     <li>f. <a href="https://static.igem.org/mediawiki/2018/4/44/T--ASIJ_Tokyo--electro.pdf">Final Polyacrylamide Gel Confirmation of Constructs </a></li>
+
     <li>h. <a href="https://static.igem.org/mediawiki/2018/4/44/T--ASIJ_Tokyo--electro.pdf">Final Polyacrylamide Gel Confirmation of Constructs </a></li>
 
</ul>
 
</ul>
  

Revision as of 15:11, 14 October 2018

EXPERIMENTS

Part I CRISPR


Part II CRISPR Confirmation (genotypic)


Part III: Designing of the new constructs (this is just modeling the construct design and not really a protocol)


Part IV: Gibson Assembly of Constructs


Part V: Deciding which fluorescent marker to use (water melon tubes, and plates)..deciding then to only use the GFP ones


Part VI: Genotypic and Phenotypic Proof of Concept

  • a. Agarose gel electrophoresis of plasmid constructs (agarose gel electorphoresis protocol)
  • b. Phenotypic Proof (Transformation and growing on selection plates)

Part VII: Quantifying Protein

Part VIII: Incorporation of New information to use His-tag