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Revision as of 15:15, 14 October 2018

EXPERIMENTS

Part I CRISPR


Part II CRISPR Confirmation (genotypic)


Part III: Designing of the new constructs (this is just modeling the construct design and not really a protocol)


Part IV: Gibson Assembly of Constructs


Part V: Deciding which fluorescent marker to use (water melon tubes, and plates)..deciding then to only use the GFP ones


Part VI: Genotypic and Phenotypic Proof of Concept

  • a. Agarose gel electrophoresis of plasmid constructs (agarose gel electorphoresis protocol)
  • b. Phenotypic Proof (Transformation and growing on selection plates)

Part VII: Quantifying Protein

Part VIII: Incorporation of New information to use His-tag