Difference between revisions of "Team:Tacoma RAINmakers/Parts"

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{{Tacoma_RAINmakers}}
 
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<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h3>Note</h3>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h3>Adding parts to the registry</h3>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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ADD PARTS
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<h3>Inspiration</h3>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
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<h1>Our Biobricks</h1>
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<h3>Parts Overview</h3>
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<p class="listy">The most significant hazard in the RAINmaker project would be the use of chemicals containing arsenic.
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Pure arsenic is not only toxic to humans, but it can also be highly toxic to aquatic life with lasting environmental and ecological effects. Sigma Aldrich warns that arsenic may also cause cancer. To work more safely, Tacoma RAINmakers used the sodium arsenate and sodium arsenite, which are the inorganic derivatives of arsenic that shows significantly less toxicity than the pure arsenic.
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When using sodium arsenate/sodium arsenite, Tacoma RAINmakers are engaging in a series of stringent safety practices. First, safety glasses will always be used to protect eyes. For hand protection, our team will wear nitrile gloves that equal or exceed required layer thickness (0.11mm). Contaminated gloves will be disposed of with proper glove removal technique (without touching glove's outer surface). To protect the body, our team will wear lab coats during all arsenic-related experiments. RAINmakers have considered air-purifying respirators, but have deemed this precaution unnecessary with proper lab technique use of a biosafety cabinet. Arsenic waste solutions or powder will not be allowed to enter drains or any location outside the lab.</p>
  
  
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<h3>Submitted Parts</h3>
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<h3>What information do I need to start putting my parts on the Registry?</h3>
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<p>The information needed to initially create a part on the Registry is:</p>
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<ul>
 
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<li>Part Name</li>
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<li class="listy">BBa_K2673000  pSB1C3/PcArsR_PArsR-spisPink</li>
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<li>Part type</li>
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<li class="listy">BBa_K2673001  pSB1C3/PcArsR_PArsR-amilCP</li>
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<li>Creator</li>
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<li class="listy">BBa_K2673002  pSB1C3/PArsR-spisPink</li>
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<li>Sequence</li>
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<li class="listy">BBa_K2673003  pSB1C3/PcArsR</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li class="listy">BBa_K2673004  pSB1C3/PArsR-amilCP</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li class="listy">BBa_K2673005 pSB1C3/T7-amilCP</li>
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<li>Design considerations</li>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h3>Part Table </h3>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<groupparts>iGEM18 Tacoma_RAINmakers</groupparts>
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Revision as of 16:59, 14 October 2018

Team:TacomaRAINmakers/Notebook - 2017.igem.org

Team:ECUST/Lab/Notebook

Our Biobricks

Parts Overview


The most significant hazard in the RAINmaker project would be the use of chemicals containing arsenic. Pure arsenic is not only toxic to humans, but it can also be highly toxic to aquatic life with lasting environmental and ecological effects. Sigma Aldrich warns that arsenic may also cause cancer. To work more safely, Tacoma RAINmakers used the sodium arsenate and sodium arsenite, which are the inorganic derivatives of arsenic that shows significantly less toxicity than the pure arsenic. When using sodium arsenate/sodium arsenite, Tacoma RAINmakers are engaging in a series of stringent safety practices. First, safety glasses will always be used to protect eyes. For hand protection, our team will wear nitrile gloves that equal or exceed required layer thickness (0.11mm). Contaminated gloves will be disposed of with proper glove removal technique (without touching glove's outer surface). To protect the body, our team will wear lab coats during all arsenic-related experiments. RAINmakers have considered air-purifying respirators, but have deemed this precaution unnecessary with proper lab technique use of a biosafety cabinet. Arsenic waste solutions or powder will not be allowed to enter drains or any location outside the lab.

Submitted Parts


  • BBa_K2673000 pSB1C3/PcArsR_PArsR-spisPink
  • BBa_K2673001 pSB1C3/PcArsR_PArsR-amilCP
  • BBa_K2673002 pSB1C3/PArsR-spisPink
  • BBa_K2673003 pSB1C3/PcArsR
  • BBa_K2673004 pSB1C3/PArsR-amilCP
  • BBa_K2673005 pSB1C3/T7-amilCP