Difference between revisions of "Team:Fudan/Attributions"

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This html document is created by Tian Huang for Team Fudan iGEM 2018.
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    <meta charset="UTF-8">
  
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    <!-- Materialize 1.0.0-rc.2 (Material Design like) -->
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<h1>Attributions</h1>
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<p>This page is your opportunity to explain what parts of your project you did and what was done by technicians, advisers, etc. This requirement is not about literature references - these can and should be displayed throughout your wiki.
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<h3> Bronze Medal Criterion #3</h3>
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<p> All of the work done in your project must be attributed correctly on this page. You must clearly state the work that was done by the students on your team and note any work that was done by people outside of your team, including the host labs, advisors, instructors, and individuals not on the team roster.
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<br><br>
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Please see the <a href="https://2018.igem.org/Judging/Medals">Medals requirements page</a> for more details.</p>
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            <!-- empty bar -->
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            <!-- Navigation bar -->
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                    <div id="teamLogo" class="brand-logo">
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                        <a href="https://2018.igem.org/Team:Fudan" target="_self"><img src="https://static.igem.org/mediawiki/2018/c/c4/T--Fudan--teamLogo.svg"></a>
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                    </div>
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                    <ul id="nav-mobile" class="right orangeBorder">
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                        <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown1">Project</a></li>
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                        <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown2">Dry lab</a></li>
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                        <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown3">Wet lab</a></li>
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                        <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown4">Toolbox</a></li>
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                        <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown5">Outreach</a></li>
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                        <li class="hide-on-med-and-down"><a class="dropdown-trigger thisPageLink" data-target="dropdown6">Team</a></li>
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                        <li>
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                            <a id="navList" data-target="slide-out" class="waves-effect waves-light sidenav-trigger right">
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                                <i class="fa fa-navicon" style="font-size: 24px"></i>
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                            </a>
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                        </li>
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                    </ul>
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                </div>
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            </nav>
  
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            <!-- Dropdown and List elements in navigation bar -->
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            <ul id="dropdown1" class="dropdown-content">
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                <li><a href="https://2018.igem.org/Team:Fudan/Demonstrate">Demonstration</a></li>
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                <li><a href="https://2018.igem.org/Team:Fudan/Results">Transmembrane logic</a></li>
 +
            </ul>
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            <ul id="dropdown2" class="dropdown-content">
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                <li><a href="https://2018.igem.org/Team:Fudan/ENABLE_ribo">Addon: <sub>ENABLE</sub><sup>ribo</sup></a></li>
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                <li><a href="https://2018.igem.org/Team:Fudan/ENABLE_TALE">Addon: <sub>ENABLE</sub><sup>TALE</sup></a></li>
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                <li><a href="https://2018.igem.org/Team:Fudan/ENABLE_T2">Addon: <sub>ENABLE</sub><sup>T2</sup></a></li>
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                <li><a href="https://2018.igem.org/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li>
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                <li><a href="https://2018.igem.org/Team:Fudan/Model#War_Predictor">Model: war&nbsp;predictor</a></li>
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                <li><a href="https://2018.igem.org/Team:Fudan/Software">Software</a></li>
 +
            </ul>
 +
            <ul id="dropdown3" class="dropdown-content">
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                <li><a href="https://2018.igem.org/Team:Fudan/InterLab">iGEM interLab</a></li>
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                <li><a href="https://2018.igem.org/Team:Fudan/Notebook">Our notebook</a></li>
 +
                <li><a href="https://2018.igem.org/Team:Fudan/Primers">Primers used</a></li>
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                <li><a href="https://2018.igem.org/Team:Fudan/Protocols">Protocols</a></li>
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                <li><a href="https://2018.igem.org/Team:Fudan/Safety">Safety</a></li>
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            </ul>
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            <ul id="dropdown4" class="dropdown-content">
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                <li><a href="https://2018.igem.org/Team:Fudan/Basic_Parts">Basic parts</a></li>
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                <li><a href="https://2018.igem.org/Team:Fudan/Composite_Parts">Composite parts</a></li>
 +
                <li><a href="https://2018.igem.org/Team:Fudan/Optimization">Optimization</a></li>
 +
                <li><a href="https://2018.igem.org/Team:Fudan/Parts_Collection">Parts collection</a></li>
 +
                <li><a href="https://2018.igem.org/Team:Fudan/Improve">Parts improvement</a></li>
 +
                <li><a href="https://2018.igem.org/Team:Fudan/Measurement">Quantification</a></li>
 +
            </ul>
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            <ul id="dropdown5" class="dropdown-content">
 +
                <li><a href="https://2018.igem.org/Team:Fudan/Bio-Art">Bio-Art display</a></li>
 +
                <li><a href="https://2018.igem.org/Team:Fudan/Collaborations">Collaborations</a></li>
 +
                <li><a href="https://2018.igem.org/Team:Fudan/Design_Intention">Design intention</a></li>
 +
                <li><a href="https://2018.igem.org/Team:Fudan/Human_Practices">Human practices</a></li>
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                <li><a href="https://2018.igem.org/Team:Fudan/Public_Engagement">Public engagement</a></li>
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            </ul>
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            <ul id="dropdown6" class="dropdown-content">
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                <li><a href="https://2018.igem.org/Team:Fudan/Acknowledgement">Acknowledgement</a></li>
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                <li><a href="https://2018.igem.org/Team:Fudan/Attributions">Attributions</a></li>
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                <li><a href="https://2018.igem.org/Team:Fudan/Heritage">Heritage</a></li>
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                <li><a href="https://2018.igem.org/Team:Fudan/Team">Members</a></li>
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                <li><a href="https://2018.igem.org/Team:Fudan/Sponsors">Sponsors</a></li>
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            </ul>
  
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<div class="column third_size">
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            <!-- Slide-out navigator contents -->
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<h3> What should this page contain?</h3>
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            <ul id="slide-out" class="sidenav">
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                <li style="padding: 0"><div class="sidenavBanner">
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                    <div class="background">
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                    </div>
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                    <p style="width: 100%;text-align: center;font-size: 24px"><span class="white-text">Attributions</span></p>
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                </div></li>
 +
                <li>
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                    <ul class="collapsible expandable">
 +
                        <li>Navigator on wiki</li>
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                        <li>
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                            <div class="collapsible-header">Project</div>
 +
                            <div class="collapsible-body">
 +
                                <ul>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Demonstrate">Demonstration</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Results">Transmembrane logic</a></li>
 +
                                </ul>
 +
                            </div>
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                        </li>
 +
                        <li>
 +
                            <div class="collapsible-header">Dry Lab</div>
 +
                            <div class="collapsible-body">
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                                <ul>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/ENABLE_ribo">Addon: <sub>ENABLE</sub><sup>ribo</sup></a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/ENABLE_TALE">Addon: <sub>ENABLE</sub><sup>TALE</sup></a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/ENABLE_T2">Addon: <sub>ENABLE</sub><sup>T2</sup></a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Model#War_Predictor">Model: war&nbsp;predictor</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Software">Software</a></li>
 +
                                </ul>
 +
                            </div>
 +
                        </li>
 +
                        <li>
 +
                            <div class="collapsible-header">Wet Lab</div>
 +
                            <div class="collapsible-body">
 +
                                <ul>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/InterLab">iGEM interLab</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Notebook">Our notebook</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Primers">Primers used</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Protocols">Protocols</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Safety">Safety</a></li>
 +
                                </ul>
 +
                            </div>
 +
                        </li>
 +
                        <li>
 +
                            <div class="collapsible-header">Toolbox</div>
 +
                            <div class="collapsible-body">
 +
                                <ul>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Basic_Parts">Basic parts</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Composite_Parts">Composite parts</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Optimization">Optimization</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Parts_Collection">Parts collection</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Improve">Parts improvement</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Measurement">Quantification</a></li>
 +
                                </ul>
 +
                            </div>
 +
                        </li>
 +
                        <li>
 +
                            <div class="collapsible-header">Outreach</div>
 +
                            <div class="collapsible-body">
 +
                                <ul>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Bio-Art">Bio-Art display</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Collaborations">Collaborations</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Design_Intention">Design intention</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Human_Practices">Human practices</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Public_Engagement">Public engagement</a></li>
 +
                                </ul>
 +
                            </div>
 +
                        </li>
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                        <li>
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                            <div class="collapsible-header">Team</div>
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                            <div class="collapsible-body">
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                                <ul>
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                                    <li><a href="https://2018.igem.org/Team:Fudan/Acknowledgement">Acknowledgement</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Attributions">Attributions</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Heritage">Heritage</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Team">Members</a></li>
 +
                                    <li><a href="https://2018.igem.org/Team:Fudan/Sponsors">Sponsors</a></li>
 +
                                </ul>
 +
                            </div>
 +
                        </li>
 +
                    </ul>
  
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<ul>
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                </li>
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<li>Clearly state what the team accomplished</li>
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                <li><div class="placeHolder"></div></li>
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<li>General Support</li>
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            </ul>
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<li>Project support and advice</li>
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        </header>
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<li>Fundraising help and advice</li>
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<li>Lab support</li>
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<li>Difficult technique support</li>
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<li>Project advisor support</li>
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<li>Wiki support</li>
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<li>Presentation coaching</li>
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<li>Human Practices support</li>
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
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</ul>
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</div>
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<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
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<ul>
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<li>Does your institution teach an iGEM or synthetic biology course?</li>
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<li>When did you start this course?</li>
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<li>Are the syllabus and course materials freely available online?</li>
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<li>When did you start your brainstorming?</li>
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<li>When did you start in the lab?</li>
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<li>When did you start working on  your project?</li>
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</ul>
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</div>
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                    <div class="col s12 hide-on-med-and-up">
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                        <h1>Attributions</h1>
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                    </div>
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                    <div class="col s12 hide-on-med-and-up">
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                        <span></span>
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                </div>
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                <div id="figureBannerTitle" class="hide-on-small-only">
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                    <h1>Attributions</h1>
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                    <p><span></span></p>
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                </div>
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                <div class="hide-on-small-only">
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                    <img src="https://static.igem.org/mediawiki/2018/d/d6/T--Fudan--public-section1-1.png">
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                    <svg width="10" height="10" xmlns="http://www.w3.org/2000/svg" style="position:absolute; left:0;top:0; width: 4%;height: 100%;">
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                        <defs>
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                            <linearGradient y2="0%" x2="100%" y1="0%" x1="0%" id="blackgraleft">
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                                <stop stop-color="rgb(0,0,0)" stop-opacity="1" offset="0%"/>
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                                <stop stop-color="rgb(0,0,0)" stop-opacity="0" offset="100%"/>
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                            </linearGradient>
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                        </defs>
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                        <g>
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                            <rect id="svg_1" fill="url(#blackgraleft)" height="100%" width="100%"/>
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                        </g>
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                    </svg>
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                    <svg width="10" height="10" xmlns="http://www.w3.org/2000/svg" style="position:absolute; right:0;top:0; width: 4%;height: 100%;">
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                        <defs>
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                            <linearGradient y2="0%" x2="100%" y1="0%" x1="0%" id="blackgraright">
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                                <stop stop-color="rgb(0,0,0)" stop-opacity="0" offset="0%"/>
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                                <stop stop-color="rgb(0,0,0)" stop-opacity="1" offset="100%"/>
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                            </linearGradient>
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                        </defs>
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                        <g>
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                            <rect id="svg_2" fill="url(#blackgraright)" height="100%" width="100%"/>
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                        </g>
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                    </svg>
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                </div>
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            </div>
  
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<h3>Inspiration</h3>
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            <!-- main content of the page -->
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<p>Take a look at what other teams have done:</p>
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            <div class="container">
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<ul>
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                <!-- side navigator of page content -->
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
+
                <main style="margin: 0;">
−
<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
+
                    <div class="section container">
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<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
+
                        <h2>Project design</h2>
−
<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
+
                        <p>
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</ul>
+
                            Zijie Yang and Dr. Cai, through discussions, came up with the first draft of <a href>3-layer</a> transmembrane logic gates with mutual effort, Ziyan Yu, through literal reading, designed the experiments to <a href>optimize</a> <a href link to the best basic part>the Notch core</a>. Everyone on the team contributed to the improvement of the project.
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</div>
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                        </p>
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</div>
+
                        <h2>Experiment</h2>
 +
                        <p>Based on <a href=2017>previous experience</a> designing genetic circuits response to different input densities, Zijie Yang and Ziyan Yu came up with <a href>the genetic circuits</a> for the project. Dr. Cai gave comments and provided several references aiding the improvement.
 +
                        </p>
 +
                        <p>Ziyan Yu, Chuyue Zhang, Yuyan Zeng, and Rongrong Du were responsible for the majority of <a href link to protocols>molecular cloning</a>. Fangfei Ye, Mitchell Wan, and Jingbo Fu assisted in some molecular cloning.
 +
                        </p>
 +
                        <p>
 +
                            Zijie and Ziyan conducted cell culture, transient transfection, and <a href link to quantification>flow cytometry analysis</a>. Zijie constructed the stable cell lines using retroviral infection and cell sorting.
 +
                        </p>
 +
                        <p>
 +
                            Rongrong purified EGFP protein, attached the protein to beads, which were used in <a href>our shaking test</a> for SynNotch activation.
 +
                        </p>
 +
                        <p>
 +
                            Dr. Cai provided numerous suggestions and advice on experimental design and data analysis.
 +
                        </p>
 +
                        <p>
 +
                            Mitchell conducted the Addon thought experiments (<a href>ribo</a>, <a href>TALE</a>, and <a href>T2</a>) with advice from Dr. Cai.
 +
                        </p>
 +
                        <h2>InterLab</h2>
 +
                        <p>
 +
                            Mitchell Wan completed <a href link to interlab>the experiments</a> with help from Zijie Yang and people from iGEM team <a href>Fudan\_China</a>.
 +
                        </p>
 +
                        <h2>Model</h2>
 +
                        <p>Yiheng Wang came up with the model on <a href>transcriptional amplifier</a>.</p>
 +
                        <p>
 +
                            Lingwei Zhang simulated <a href>multi-cell interactions and evolution</a> with a population perspective. Lingwei also built our <a href>software</a> based on his modeling results.
 +
                        </p>
 +
                        <p>
 +
                            Zijie Yang and Yijie Pan provided suggestions to different details in our modeling.
 +
                        </p>
 +
                        <h2>Software</h2>
 +
                        <p>
 +
                            Lingwei Zhang wrote up our <a href>software</a>.
 +
                        </p>
 +
                        <h2>Integrated HP</h2>
 +
                        <p>
 +
                            Jingbo Fu, Yuyan Zeng, Fangfei Ye, and Rongrong Du made huge efforts in advertising our project and <a href>human practice activities</a> to the public.
 +
                        </p>
 +
                        <p>
 +
                            Jingbo led the <a href link to section>surveys</a>, organized the <a href link to section>interviews</a> with researchers and doctors. Everyone on the team heartily helped and participated in various HP events.
 +
                        </p>
 +
                        <p>
 +
                            Zijie Yang and Ziyan Yu invited Prof. Blacklow to Fudan University, and accompany his visit.
 +
                        </p>
 +
                        <p>
 +
                            Jingbo and Yuyan kept in contact with <a href link to section the first mentioned school>high schools</a>, organizers responsible for <a href link to section>the summer camp</a>, and other undergraduate students. They led our presentations for them, and everyone on the team participated actively.
 +
                        </p>
 +
                        <p>
 +
                            Jingbo designed and <a href link to section>trained</a> high school students from Qibao Dwight High School during this summer, also organized the multi-disciplinary <a href link to section>debate</a> on project safety and ethical topics.
 +
                        </p>
 +
                        <p>
 +
                            Fangfei planned and assembled the <a href>Bio-Art Display</a> and everyone on the team took part in making the Display come true.
 +
                        </p>
 +
                        <h2>Art design</h2>
 +
                        <p>
 +
                            Fangfei Ye is responsible for all <a href link to page>art design</a>, which includes our team logo, team flag, team uniform (Dr. Cai gave comments), team name card, brochures, our posters, as well as materials related to our human practice events.
 +
                        </p>
 +
                        <h2>Wiki</h2>
 +
                        <p>
 +
                            Tian Huang built the wiki template with Materialize framework. Fangfei Ye and Tian coded the site, while rest of the team worked on content write-up. Dr. Cai gave comments on the coding and writeup.
 +
                        </p>
 +
                        <h2>Support</h2>
 +
                        <p>
 +
                            Dr. Lu and Dr. Cai secured the primary funding for the team. Dr. Cai provided the lab space. All team members participated in the crowdfunding campaign.</p>
  
 +
                    </div>
  
 +
                </main>
 +
            </div>
  
−
<div class="clear extra_space"></div>
+
            <!--Abstract on content page-->
 +
            <div id="abstractContent" class="z-depth-2">
 +
                <a href="#!"><img src="https://static.igem.org/mediawiki/2018/9/96/T--Fudan--X.svg"></a>
 +
                <div class="container">
 +
                    <h2 style="margin: 0;padding: 10px 0;">Abstract</h2>
 +
                    <p style="margin: 0">Contact-dependent signaling is critical for multicellular biological
 +
                        events, yet customizing contact-dependent signal transduction between
 +
                        cells remains challenging. Here we have developed the ENABLE toolbox, a
 +
                        complete set of transmembrane binary logic gates. Each gate consists of
 +
                        3 layers: Receptor, Amplifier, and Combiner. We first optimized synthetic
 +
                        Notch receptors to enable cells to respond to different signals across the
 +
                        membrane reliably. These signals, individually amplified intracellularly by
 +
                        transcription, are further combined for computing. Our engineered zinc finger-based
 +
                        transcription factors perform binary computation and output designed products.
 +
                        In summary, we have combined spatially different signals in mammalian cells,
 +
                        and revealed new potentials for biological oscillators, tissue engineering,
 +
                        cancer treatments, bio-computing, etc. ENABLE is a toolbox for constructing
 +
                        contact-dependent signaling networks in mammals. The 3-layer design principle
 +
                        underlying ENABLE empowers any future development of transmembrane logic circuits,
 +
                        thus contributes a foundational advance to Synthetic Biology.
 +
                    </p>
 +
                </div>
 +
            </div>
  
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<div class="column two_thirds_size">
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            <!-- Floating Btns -->
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<h3> Why is this page needed? </h3>
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            <div class="floatingBtn">
−
<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
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                <a href="#!" id="abstractBtn" class="btn">
−
<p>
+
                    <i class="fa fa-sticky-note" style="font-size: 30px;line-height: 50px"></i>
−
For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
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                <div class="container">
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                        <div id="sponsor" class="col m3 s12 row">
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                            <a href="https://2018.igem.org/Team:Fudan" target="_blank"><img class="col s3 m6 l3" style="position:relative; padding: 0 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2018/7/73/T--Fudan--teamLogoWhite.png">
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                            </a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png">
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                        </a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* 该图比其他小一点，排版需要 */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png">
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                        </a><a href="http://www.yfc.cn/en/" target="_blank"><img class="col s3 m6 l3" style="padding: 0.15rem 0.9rem;" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2018/e/e2/T--Fudan--yunfengLogo.png">
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                        </a>
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                            <h3 class="col s12" style="text-align: left; color: rgba(255, 255, 255, 0.8); font-size: 18px">ENABLE: making cells even smarter</h3>
 +
                        </div>
 +
                        <div id="usefulLinks" class="col m9 s12 row">
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                            <div class="col s12 l6 row">
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                                <div class="col s12 m4">
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                                    <span>Project</span>
 +
                                    <ul>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Demonstrate">Demonstration</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Results">Transmembrane logic</a></li>
 +
                                        <li><a href="https://2017.igem.org/Team:Fudan">2017.iGEM</a></li>
 +
                                    </ul>
 +
                                </div>
 +
                                <div class="col s12 m4">
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                                    <span>Dry lab</span>
 +
                                    <ul>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/ENABLE_ribo">Addon: <sub>ENABLE</sub><sup>ribo</sup></a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/ENABLE_TALE">Addon: <sub>ENABLE</sub><sup>TALE</sup></a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/ENABLE_T2">Addon: <sub>ENABLE</sub><sup>T2</sup></a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Model#War_Predictor">Model: war&nbsp;predictor</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Software">Software</a></li>
 +
                                    </ul>
 +
                                </div>
 +
                                <div class="col s12 m4 active">
 +
                                    <span>Wet lab</span>
 +
                                    <ul>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/InterLab">iGEM interLab</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Notebook">Our notebook</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Primers">Primers used</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Protocols">Protocols</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Safety">Safety</a></li>
 +
                                    </ul>
 +
                                </div>
 +
                            </div>
 +
                            <div class="col s12 l6 row">
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                                <div class="col s12 m4">
 +
                                    <span>Toolbox</span>
 +
                                    <ul>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Basic_Parts">Basic parts</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Composite_Parts">Composite parts</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Optimization">Optimization</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Parts_Collection">Parts collection</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Improve">Parts improvement</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Measurement">Quantification</a></li>
 +
                                    </ul>
 +
                                </div>
 +
                                <div class="col s12 m4">
 +
                                    <span>Outreach</span>
 +
                                    <ul>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Bio-Art">Bio-Art display</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Collaborations">Collaborations</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Design_Intention">Design intention</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Human_Practices">Human practices</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Public_Engagement">Public engagement</a></li>
 +
                                    </ul>
 +
                                </div>
 +
                                <div class="col s12 m4">
 +
                                    <span>Team</span>
 +
                                    <ul>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Acknowledgement">Acknowledgement</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Attributions">Attributions</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Heritage">Heritage</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Team">Members</a></li>
 +
                                        <li><a href="https://2018.igem.org/Team:Fudan/Sponsors">Sponsors</a></li>
 +
                                    </ul>
 +
                                </div>
 +
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Revision as of 01:52, 15 October 2018

Attributions

Attributions

Project design

Zijie Yang and Dr. Cai, through discussions, came up with the first draft of 3-layer transmembrane logic gates with mutual effort, Ziyan Yu, through literal reading, designed the experiments to optimize the Notch core. Everyone on the team contributed to the improvement of the project.

Experiment

Based on previous experience designing genetic circuits response to different input densities, Zijie Yang and Ziyan Yu came up with the genetic circuits for the project. Dr. Cai gave comments and provided several references aiding the improvement.

Ziyan Yu, Chuyue Zhang, Yuyan Zeng, and Rongrong Du were responsible for the majority of molecular cloning. Fangfei Ye, Mitchell Wan, and Jingbo Fu assisted in some molecular cloning.

Zijie and Ziyan conducted cell culture, transient transfection, and flow cytometry analysis. Zijie constructed the stable cell lines using retroviral infection and cell sorting.

Rongrong purified EGFP protein, attached the protein to beads, which were used in our shaking test for SynNotch activation.

Dr. Cai provided numerous suggestions and advice on experimental design and data analysis.

Mitchell conducted the Addon thought experiments (ribo, TALE, and T2) with advice from Dr. Cai.

InterLab

Mitchell Wan completed the experiments with help from Zijie Yang and people from iGEM team Fudan\_China.

Model

Yiheng Wang came up with the model on transcriptional amplifier.

Lingwei Zhang simulated multi-cell interactions and evolution with a population perspective. Lingwei also built our software based on his modeling results.

Zijie Yang and Yijie Pan provided suggestions to different details in our modeling.

Software

Lingwei Zhang wrote up our software.

Integrated HP

Jingbo Fu, Yuyan Zeng, Fangfei Ye, and Rongrong Du made huge efforts in advertising our project and human practice activities to the public.

Jingbo led the surveys, organized the interviews with researchers and doctors. Everyone on the team heartily helped and participated in various HP events.

Zijie Yang and Ziyan Yu invited Prof. Blacklow to Fudan University, and accompany his visit.

Jingbo and Yuyan kept in contact with high schools, organizers responsible for the summer camp, and other undergraduate students. They led our presentations for them, and everyone on the team participated actively.

Jingbo designed and trained high school students from Qibao Dwight High School during this summer, also organized the multi-disciplinary debate on project safety and ethical topics.

Fangfei planned and assembled the Bio-Art Display and everyone on the team took part in making the Display come true.

Art design

Fangfei Ye is responsible for all art design, which includes our team logo, team flag, team uniform (Dr. Cai gave comments), team name card, brochures, our posters, as well as materials related to our human practice events.

Wiki

Tian Huang built the wiki template with Materialize framework. Fangfei Ye and Tian coded the site, while rest of the team worked on content write-up. Dr. Cai gave comments on the coding and writeup.

Support

Dr. Lu and Dr. Cai secured the primary funding for the team. Dr. Cai provided the lab space. All team members participated in the crowdfunding campaign.

Abstract

Contact-dependent signaling is critical for multicellular biological events, yet customizing contact-dependent signal transduction between cells remains challenging. Here we have developed the ENABLE toolbox, a complete set of transmembrane binary logic gates. Each gate consists of 3 layers: Receptor, Amplifier, and Combiner. We first optimized synthetic Notch receptors to enable cells to respond to different signals across the membrane reliably. These signals, individually amplified intracellularly by transcription, are further combined for computing. Our engineered zinc finger-based transcription factors perform binary computation and output designed products. In summary, we have combined spatially different signals in mammalian cells, and revealed new potentials for biological oscillators, tissue engineering, cancer treatments, bio-computing, etc. ENABLE is a toolbox for constructing contact-dependent signaling networks in mammals. The 3-layer design principle underlying ENABLE empowers any future development of transmembrane logic circuits, thus contributes a foundational advance to Synthetic Biology.