Difference between revisions of "Team:US AFRL CarrollHS/InterLab"
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| − | + | <div class="row"><p>This year, we complete the Interlab along with the Flow Cytometry portion to qualify for the bronze medal. Below you can find our data including the results of our competent cell test kit, background fluorescence information from out samples, and raw data. This year we were also able to utilize our Flow Cytometer as well to submit our flow cytometry data to iGEM. We hope that by submitting our data to iGEM, we can help standardize measuring protocols for reproducibility within experiments.</p> | |
| + | <p>In this year’s InterLab, our team transformed E. coli DH5α with different plasmids expressing Green Fluorescent Protein (GFP). A plate reader was then used to measure fluorescence and absorbance values from the transformed strains. </p></div> | ||
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| − | < | + | <div class="row"><h1>Competent Cells Test</h1></div> |
| − | < | + | <p><b>INSERT INFO HERE</b></p> |
| + | <!--PICTUREs--> | ||
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| + | <div class="row"><h1>Results</h1></div> | ||
| + | <div class="row"><h2>Calibration 1: OD600 Reference point with LUDOX Protocol</h2></div> | ||
| + | <div class="row"><h2>Calibration 2: Particle Standard Curve with Microsphere Protocol</h2></div> | ||
| + | <div class="row"><h2>Calibration 3: Fluorescence standard curve with Fluorescence Protocol</h2></div> | ||
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| + | <div class="row"><h1>Cell Measurement Protocol</h1></div> | ||
| + | <div class="row"><h2>Day One</h2></div> | ||
| + | <div class="row"><h2>Day Two</h2></div> | ||
| + | <div class="row"><h2></h2>Day Three</div> | ||
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Revision as of 03:23, 15 October 2018
This year, we complete the Interlab along with the Flow Cytometry portion to qualify for the bronze medal. Below you can find our data including the results of our competent cell test kit, background fluorescence information from out samples, and raw data. This year we were also able to utilize our Flow Cytometer as well to submit our flow cytometry data to iGEM. We hope that by submitting our data to iGEM, we can help standardize measuring protocols for reproducibility within experiments.
In this year’s InterLab, our team transformed E. coli DH5α with different plasmids expressing Green Fluorescent Protein (GFP). A plate reader was then used to measure fluorescence and absorbance values from the transformed strains.
Competent Cells Test
INSERT INFO HERE
Results
Calibration 1: OD600 Reference point with LUDOX Protocol
Calibration 2: Particle Standard Curve with Microsphere Protocol
Calibration 3: Fluorescence standard curve with Fluorescence Protocol