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| | <div style="background: url(https://static.igem.org/mediawiki/2018/d/d6/T--SHSID_China--main_bg2.jpg); background-size: cover; background-position: center" class="link" id="Abstract"> | | <div style="background: url(https://static.igem.org/mediawiki/2018/d/d6/T--SHSID_China--main_bg2.jpg); background-size: cover; background-position: center" class="link" id="Abstract"> |
| − | <article style="color: white; font-size: 16px; padding-top: 10px"> | + | <article> |
| − | <div class="column full_size">
| + | <h2 style="color: white; font-family: 'Trocchi', serif;">1. Collaboration with SHSU_China</h2> |
| − | <h1>Collaborations</h1>
| + | <h3 style="color: white; font-family: 'Trocchi', serif;">a) What they did for us</h3> |
| − | | + | <p style="color: white; font-size: 16px; padding-top: 10px"> <strong>DNA Kit Plate. </strong> The SHSU_China team provided our team with their DNA Kit Plate 1 after we used up all the plasmid in well 4L (part BBa_K325909 which contains the pSB1C3-Lux plasmid) for transformation that unexpectedly ended up in failure.</p> |
| − | Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.
| + | <p style="color: white; font-size: 16px; padding-top: 10px"><strong>Ligation.</strong> They helped us ligate 2×20μL of our part BBa_K2836001 (pSB1C3-LuxG) using a Quick Ligation system from New England BioLabs (with the Quick Ligation Enzyme, Stock No. M2200), as this system can complete the entire ligation process in 10-15 minutes.</p> |
| − | </p>
| + | <p style="color: white; font-size: 16px; padding-top: 10px"><strong>Competent cell.</strong> They provided us with three tubes of <em>E. coli</em> DH5-α competent cell after our lab ran out when we needed to transform our BBa_K2836001.</p> |
| − | | + | <p style="color: white; font-size: 16px; padding-top: 10px"><strong>Troubleshooting.</strong> When one of the forms in our first submission for the InterLab was rejected, SHSU_China and our team worked together to find any errors in our submission. Eventually, we found out that it was only a misinterpretation of the requirements (there was a section where we multiplied our Abs600 values with the conversion factor which was unnecessary). With their help, our second submission of the InterLab was successful.</p> |
| − | <h3>Silver Medal Criterion #2</h3>
| + | <h3 style="color: white; font-family: 'Trocchi', serif;">b) What we did for them</h3> |
| − | <p>
| + | <p style="color: white; font-size: 16px; padding-top: 10px"> <strong>Laboratory and equipment.</strong> Thanks to their help, we gladly shared our lab and equipment with the SHSU_China team in order for them to complete their InterLab study. Their laboratory lacked a suitable equipment to measure absorbance and fluorescence in a 96-well plate. We also utilized this chance to aid each other through the entire process of InterLab, helping out during experiments and eventually comparing our results.</p> |
| − | Complete this page if you intend to compete for the silver medal criterion #2 on collaboration. Please see the <a href="https://2018.igem.org/Judging/Medals">2018 Medals Page</a> for more information.
| + | <p style="color: white; font-size: 16px; padding-top: 10px"><strong>Transformation.</strong> We helped SHSU_China transform and amplify part BBa_K1321200 from well 16E on DNA Kit Plate 5 which contains their desired VHb protein.</p> |
| − | </p>
| + | <h2 style="color: white; font-family: 'Trocchi', serif;">2. High Schools in Shanghai Meetup</h2> |
| − | </div>
| + | <h3 style="color: white; font-family: 'Trocchi', serif;">a) Met with SHSU_China and SHPH-Shanghai at Fudan University on October 14, 2018</h3> |
| − | | + | <h3 style="color: white; font-family: 'Trocchi', serif;">b) Gave mock presentations to prepare for the actual presentation at the Giant Jamboree</h3> |
| − | <div class="column two_thirds_size">
| + | <h3 style="color: white; font-family: 'Trocchi', serif;">c) Gave feedback on each others’ presentation</h3> |
| − | | + | <h3 style="color: white; font-family: 'Trocchi', serif;">d) Shared experiences with regards to human practices and modelling</h3> |
| − | <h4> Which other teams can we work with? </h4>
| + | <img src="https://static.igem.org/mediawiki/2018/a/a8/T--SHSID_China--collab1.png" style="margin-left: auto; margin-right: auto; width: 50em; text-align: center; display: block"> |
| − | <p>
| + | <img src="https://static.igem.org/mediawiki/2018/6/69/T--SHSID_China--collab2.png" style="margin-left: auto; margin-right: auto; width: 50em; text-align: center; display: block"> |
| − | You can work with any other team in the competition, including software, hardware, high school and other tracks. You can also work with non-iGEM research groups, but they do not count towards the iGEM team collaboration silver medal criterion.
| + | |
| − | </p>
| + | |
| − | | + | |
| − | | + | |
| − | In order to meet the silver medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.
| + | |
| − | </p>
| + | |
| − | | + | |
| − | </div>
| + | |
| − | <div class="column third_size">
| + | |
| − | <p>
| + | |
| − | Here are some suggestions for projects you could work on with other teams:
| + | |
| − | </p>
| + | |
| − | | + | |
| − | <ul> | + | |
| − | <li> Improve the function of another team's BioBrick Part or Device</li>
| + | |
| − | <li> Characterize another team's part </li>
| + | |
| − | <li> Debug a construct </li>
| + | |
| − | <li> Model or simulate another team's system </li>
| + | |
| − | <li> Test another team's software</li>
| + | |
| − | <li> Help build and test another team's hardware project</li>
| + | |
| − | <li> Mentor a high-school team</li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| | </article> | | </article> |
| | </div> | | </div> |
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