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| | + | <h2>Our theme: </h2> |
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| | + | <p>CyanoElimination: The lysis of Cyanobacteria in freshwater ecosystem using Cyanophage lysozyme and its commercial implications.</p> |
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| | + | <h2>Let us introduce you to....</h2> |
| | + | <p>The rampant growth of cyanobacteria in freshwater ecosystem has become more than an environmental issue. Their incredible ability to multiply and voracious consumption of oxygen often make them a disturbing factor to natural systems. We realized during <a href='https://2018.igem.org/Team:SBS_SH_112144/Human_Practices'>public outreach</a > that although effective ways to gather and salvage cyanobacteria have been developed, there are barely any success in decomposing these bacterials. |
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| | + | Our team identified a cyanophage lysozyme, cp-OS lysozyme 1. Alone with other chemicals such as bugbuster, this lysozyme in small reaction systems could lyse the cyanobacteria effectively. Through a series of experiments, we were able to acquire the recombinant protein from E. coli cells. Our Modeling helped us finding the best experimental conditions for a prototype device in which would contribute to a system of cyanobacteria elimination. Our research lays foundation for the utilization of cyanobacteria components in agricultural, bioenergetic, and even medical fields.</p> |
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| − | <h1> Welcome to iGEM 2018! </h1>
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| − | <p>Your team has been approved and you are ready to start the iGEM season! </p>
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| − | <p>Cyanobacteria release microcystin LR(MCLR), the most common of microcystin(MC), which is toxic to human and can lead to severe diseases such as liver cancer(Shimizu 2011). Massive reproduction of cyanobacteria has occurred throughout the world and caused serious water quality problem, such as the case of Taihu Lake in 2007. MCLR are structurally stable to withstand physicochemical and biological stresses, including pH, temperature, sunlight and common enzymes, under natural conditions (Harada 1996; Okano et al. 2006). Neither can common water filtering process successfully get rid of it, meaning that only those natural MC-degrading bacterias is capable of degrading MCLR. Studying the degradation process is thus critical to address the water quality problem brought by MCLR. Among 10-more species of such bacteria, our group intends to research on the strand Sphingomonas sp, which possess a MC-degrading pathway that involves 3 genes: mrl A mrl B mrl C that works in a sequential chain reaction(Shimizu 2011) and an additional gene, mrl D, whose product is speculated to aid the transport of MCLR or its degradation process(Bourne, 2001). The MRL A enzyme first linearize the ring-form MCLR, then MRLB cut it into Tetrapeptide, following by the further hydrolysis of Tetra-Peptide into smaller peptides, such as Adda, illustrated by the graph below.(Bourne, 2001). Previous studies have also shown that MCLR can induce the expression of all 3 genes, while linearized MCLR,tetrapeptide and Adda only stimulate the expression of mlr A and mlrB (Shimizu 2011). Being familiar with these information, our group aims to explore on how different combination of these genes can affect the MCLR degradation rates, so that a more efficient degrading model mabe be established.</p>
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| − | <li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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| − | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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| − | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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| − | <h3> Uploading pictures and files </h3>
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| − | <p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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| − | <p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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| − | <p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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| − | <p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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| − | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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| − | <li>Have lots of fun! </li>
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| − | <h3>Inspiration</h3>
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| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p>
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| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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CyanoElimination: The lysis of Cyanobacteria in freshwater ecosystem using Cyanophage lysozyme and its commercial implications.
The rampant growth of cyanobacteria in freshwater ecosystem has become more than an environmental issue. Their incredible ability to multiply and voracious consumption of oxygen often make them a disturbing factor to natural systems. We realized during public outreach that although effective ways to gather and salvage cyanobacteria have been developed, there are barely any success in decomposing these bacterials.
Our team identified a cyanophage lysozyme, cp-OS lysozyme 1. Alone with other chemicals such as bugbuster, this lysozyme in small reaction systems could lyse the cyanobacteria effectively. Through a series of experiments, we were able to acquire the recombinant protein from E. coli cells. Our Modeling helped us finding the best experimental conditions for a prototype device in which would contribute to a system of cyanobacteria elimination. Our research lays foundation for the utilization of cyanobacteria components in agricultural, bioenergetic, and even medical fields.
