Description

1. Nicotine (1-methyl-2-[3-pyridyl-pyrrolidine], C10H14N2), a major alkaloid in tobacco plants,is a significant factor of evaluation for tobacco and cigarettes. Nicotine plays a critical role in smoking addiction and is well known to be harmful to human beings, because it easily crosses the blood-brain barrier and biological membranes. Meanwhile, with large quantities of tobacco products being produced and consumed, tobacco waste, which includes many toxic substances such as nicotine, aminobiphenyl, naphthylamine, and benzo(a)- pyrene, is entering the environment. Moreover, a number of toxic and harmful substances containing nicotine and other alkaloids are generated in tobacco processing and cannot be recycled, producing serious environmental problems. 配个图片，以上文字主要说尼古丁对人体和环境的污染。

2. In recent years, more nicotine-degrading bacteria have been reported, and the degradation pathways of nicotine have been elucidated. The degradation of nicotine through the pyrrolidine pathway by Pseudomonas has been extensively studied. 图片添加：尼古丁酶催化降解途径的图，要是清楚的话可以通过chemdraw画一个

3、In our study， the first three gene_____，_____and_____(增加三个基因的名字和基因序列号) is crucial for nicotine degradation, so we will construct a pathway to express the three gene in top10. 图片添加：载体构建图和实验流程图

基因路线图中的汉语的翻译分别是 Nicotine degradation Gene cloning Pathway construction Vector construction Protein expression Screening