Difference between revisions of "Team:Nanjing-China/Demonstrate"

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      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Demonstrate">Demonstrate</a></li></ul></div>
 
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      <ul>
 
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      <li><a href="#overview">Overview</a></li>
 
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      <li><a href="#cds">CdS</a></li>
 
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      <li><a href="#nit"><font size="-1">Nitrogen fixation</font></a></li>
 
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      <li><a href="#device">Device</a></li>
 
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        <ul>
 
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  <li><a href="https://2018.igem.org/Team:Nanjing-China">PEOPLE</a>
 
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    <ul>
 
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Team">Team</a></li>
 
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Members">Members</a></li>
 
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Attributions">Attributions</a></li>
 
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/For_Judges">For_Judges</a></li>
 
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Background">PROJECT</a>
 
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    <ul>
 
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Background">Background</a></li>
 
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Design">Design</a></li>
 
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Results">Results</a></li>
 
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Demonstrate">Demonstrate</a></li>
 
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Hardware">Hardware</a></li> 
 
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/InterLab">InterLab</a></li>
 
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Parts">PARTS</a>
 
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Basic_Part">Basic_Part</a></li>
 
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Composite_Part">Composite</a></li>
 
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Improve">Improve</a></li>
 
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Model">MODEL</a>
 
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices">PRACTICES</a>
 
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></li>
 
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Collaborations">Collaboration</a></li>
 
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Notebook">NOTEBOOK</a></li>
 
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      <h2>Overview</h2>
 
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        <p>This year, we found  an economic, efficient and environmental-friendly way to utilize nitrogen in  the air. Our design consists of three parts: biosynthesis of CdS semiconductor, light-driven  nitrogen fixation and reaction device. All three parts have gone  through in-depth examination and successfully work out under real conditions.<br />
 
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        The following list exhibits our key  achievements in this project.<br />
 
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        Constructed and  tested engineered <em>E. coli</em> which express nitrogenase and OmpA-PbrR. <br />
 
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        Constructed and  tested light-driven system based on OmpA-PbrR protein.<br />
 
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        Achieved  light-driven nitrogen fixation.<br />
 
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        Designed a reaction  device that cater for our light-driven nitrogen fixation reaction.</p>
 
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      <h3>Biosynthesis  of CdS semiconductor</h3>
 
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      <p>One key element  of light-driven system in our design is CdS semiconductor precipitated by the OmpA-PbrR  fused protein. We used TEM-EDX analysis to characterize CdS semiconductor  precipitated on E. coli cell. We also determined the maximum concentration of  Cd2+ appropriate for strain growth, as well as the amount of CdS  that can be precipitated on cell surface.</p>
 
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      <h3>Light-driven  nitrogen fixation</h3>
 
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        <p>CdS  semiconductor generate excited electrons under illumination which are then  passed to nitrogenase for nitrogen fixation. We introduce nitrogenase to <em>E.coli</em> to enable it to reduce dinitrogen to ammonia. We conducted QPCR to detect  relative transcriptional level of each <em>nif</em> gene. We also plan to optimize the structure of <em>nif </em>gene operon after modeling. Using acetylene reduce assay, we then  verify the activity of nitrogenase in our system.</p>
 
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      <h3>Reaction  device</h3>
 
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        <p>We designed a light-driven microbe reaction  device to apply our system to practical use. After a few test, we proved our  device to be quite practical. <a href="https://2018.igem.org/Team:Nanjing-China/Hardware">(see device for more details)</a></p>
 
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        <div class="f-word"><p><strong><u>Address</u></strong></p>
 
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            <p>Life Science Department<br />
 
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              #163 Xianlin Blvd, Qixia District<br />
 
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              Nanjing University<br />
 
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              Nanjing, Jiangsu Province<br />
 
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              P.R. of China<br />
 
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              Zip: 210046
 
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            </p></div>
 
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            <p><strong><u>Email</u></strong></p>
 
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            <p>nanjing_china@163.com</p>
 
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            <p><strong><u>Social media</u></strong></p>
 
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            <p>Twitter button: <br/>
 
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            <a href="https://twitter.com/Guja1501">iGEM Nanjing-China@nanjing_china</a></p>
 
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Revision as of 09:03, 15 October 2018

Nanjing-China2018