Difference between revisions of "Team:NUS Singapore-A/shadow/Composite Part"

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<!-- CHARACTERIZATION OF STRESS PROMOTER -->
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<h1>OVERVIEW</h1>
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   <h1> EXPERIMENTS </h1>
 
   <h1> EXPERIMENTS </h1>

Revision as of 11:13, 15 October 2018

CONNECT WITH US

OVERVIEW

To write an overview.


To continue writing something else.





LIST OF COMPOSITE PARTS

Device Part Number Usage
PhtpG1-mRFP BBa_K2819118 Stress reporter
NANDA FILL ME BBa_I20270 Promoter MeasKit (J23151)


OVERVIEW

To write an overview.


To continue writing something else.



EXPERIMENTS

Abs600

  • Wavelength: 600nm
  • Read Speed: Normal
  • Delay: 100 msec

Fluorescence

  • Excitation: 485
  • Emission: 525
  • Optics: Top
  • Gain: 50
  • Light Source: Xenon Flash
  • Lamp Energy: High
  • Read Speed: Normal
  • Delay: 100 msec
  • Read Height: 7 mm

You can put text here if you wanna

CONCLUSION


The results from our experiment seem to indicate that normalizing fluorescence measurements to absolute cell count using the Study’s two methods will not be able to reduce lab-to-lab variability because counting colony-forming units do not return the expected cell concentration, i.e. the cell concentration modeled by the silica beads in Method 1. While both methods cannot be used independently to establish a robust fluorescence measurement system, it may be possible that lab-to-lab variability can be reduced if a different method of normalizing to absolute cell count is devised, replacing Method 1, Method 2, or both.