Difference between revisions of "Team:JNFLS/Safety"

 
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<h1> Safety </h1>
 
<h1> Safety </h1>
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<p>Biosafety is the prevention of exposure to biohazards or pathogen dissemination, concerning on the issues of ecology and hygiene. Human errors, unqualified instruments, poor experimental techniques would all possibly contribute to deficient safety insurance. Typical topics in biosafefy involve the regular conduction of examinations on laboratory settings and machines, a set of strict laboratory guidelines to obey, etc. Working with synthetic biology experiment, especially high-security apparatus and facilities are required to prevent harmful chemicals or engineered organisms from being released accidentally. </p>
 
<p>Biosafety is the prevention of exposure to biohazards or pathogen dissemination, concerning on the issues of ecology and hygiene. Human errors, unqualified instruments, poor experimental techniques would all possibly contribute to deficient safety insurance. Typical topics in biosafefy involve the regular conduction of examinations on laboratory settings and machines, a set of strict laboratory guidelines to obey, etc. Working with synthetic biology experiment, especially high-security apparatus and facilities are required to prevent harmful chemicals or engineered organisms from being released accidentally. </p>
 
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<h3>Safe Project Design</h3>
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<h2>Following promise</h2>
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<h3>Biosafety Level of Our Laboratory</h3>
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<p>With the guidance from iGEM competition and locally safety regulation, and in order to keep our members from bio risks and correspond to the regulations above, our laboratory is ranked as BSL-1. Biosafety Level 2 laboratory generally deal with applicable microorganisms without harmful pathogenic potential on healthy adults. Additionally, all of the parts and species we utilized, which are listed in the chart below, are environmental friendly, not potentially harmful or pathogenic to healthy adult individuals.Before entering the labortary, everyone can notoce the reminders as follows: </p>
  
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<img src="https://static.igem.org/mediawiki/2018/8/88/T--JNFLS--S1.jpg"style="width:35%">
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<li>Choosing a non-pathogenic chassis</li>
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<img src="https://static.igem.org/mediawiki/2018/4/44/T--JNFLS--SA2.jpg"style="width:40%">
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<h3>Lab Equipment</h3>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<p>Our lab is equipped with advanced instruments to ensure safety, including laminar flow hood, autoclaves, and personal protective equipment. We also set a trash can especially for polluted wastes.And we have a medical box contains some available medical items at any time.</p>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<img src="https://static.igem.org/mediawiki/2018/a/a1/T--JNFLS--SA3.jpg"style="width:40%">
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<img src="https://static.igem.org/mediawiki/2018/1/1c/T--JNFLS--SA4.jpg"style="width:40%">
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<img src="https://static.igem.org/mediawiki/2018/4/4c/T--JNFLS--S8.jpg"style="width:44%">
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<img src="https://static.igem.org/mediawiki/2018/1/1d/T--JNFLS--S4.jpg"style="width:40%">
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<h3>Experiment operation</h3>
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<p>During the entire experiment, each member strictly followed the criteria as presented:</p>
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<ul>  
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<li>Fire, electric heaters and microwaves should not be left unheeded.Our lab is also well equipped with fire hydrant, fire extinguishers and fire blankets to make sure the fire could be extinguished within a short period of time. Our safety instructor has taught everyone how to use these extinguishing equipment. We have also studied the using method of alcohol lamp and other essential safety procedures. </li>
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<img src="https://static.igem.org/mediawiki/2018/7/72/T--JNFLS--S5.jpg"style="width:45%">
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<img src="https://static.igem.org/mediawiki/2018/b/b0/T--JNFLS--S3.jpg"style="width:45%">
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<li>Bacteria should be operated in laminar flow hood to secure isolated environment</li>
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<li>Wastes containing living organisms should be autoclaved before throwing away and sent to certain institution for disposal</li>
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<li>Glass apparatus should be disinfected regularly</li>
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<li>Rubber gloves, lab coats, long pants and shoes should be equipped to cover the entire skin when conducting experiment</li>
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<li>Water, fire, electricity and air conditioner should be examined whenever an experiment is suspended</li>
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<li>The laboratory should be cleaned at least once a week</li>
 
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<h3>Safety tutoring</h3>
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<p>Before our team members commenced on the experiment, we have been trained with safety issues systematically.For example, we have been taught how to use extinguishers and fire blankets for in case, also we ‘ve learned the rules of waste cleaning and made a schedule for it.  We give sincere thanks to Professor Ren from Shandong University for offering the training session.</p>
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<img src="https://static.igem.org/mediawiki/2018/8/81/T--JNFLS--SA5.jpg"style="width:45%">
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<img src="https://static.igem.org/mediawiki/2018/9/98/T--JNFLS--oo.jpg"style="width:45%">
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<h3>Safety concerns of our project</h3>
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<p>Our project aims to increase the sensitivity and specificity on the testing for HCV in blood. In the future, we will request a department which is qualified with Biosafety Level 3 to test our system using HCV infected serum. If it is applicable after further evaluation, there will be a significant decrease in the possibility that patients would received infected blood. Thus, our project will add insurance to public hygiene with higher efficiency and lower cost on HCV detection.
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<h3>Safe Lab Work</h3>
 
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
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<h3>Safe Shipment</h3>
 
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
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Latest revision as of 13:27, 15 October 2018

Safety

Biosafety is the prevention of exposure to biohazards or pathogen dissemination, concerning on the issues of ecology and hygiene. Human errors, unqualified instruments, poor experimental techniques would all possibly contribute to deficient safety insurance. Typical topics in biosafefy involve the regular conduction of examinations on laboratory settings and machines, a set of strict laboratory guidelines to obey, etc. Working with synthetic biology experiment, especially high-security apparatus and facilities are required to prevent harmful chemicals or engineered organisms from being released accidentally.

Following promise

Biosafety Level of Our Laboratory

With the guidance from iGEM competition and locally safety regulation, and in order to keep our members from bio risks and correspond to the regulations above, our laboratory is ranked as BSL-1. Biosafety Level 2 laboratory generally deal with applicable microorganisms without harmful pathogenic potential on healthy adults. Additionally, all of the parts and species we utilized, which are listed in the chart below, are environmental friendly, not potentially harmful or pathogenic to healthy adult individuals.Before entering the labortary, everyone can notoce the reminders as follows:

Lab Equipment

Our lab is equipped with advanced instruments to ensure safety, including laminar flow hood, autoclaves, and personal protective equipment. We also set a trash can especially for polluted wastes.And we have a medical box contains some available medical items at any time.

Experiment operation

During the entire experiment, each member strictly followed the criteria as presented:

  • Fire, electric heaters and microwaves should not be left unheeded.Our lab is also well equipped with fire hydrant, fire extinguishers and fire blankets to make sure the fire could be extinguished within a short period of time. Our safety instructor has taught everyone how to use these extinguishing equipment. We have also studied the using method of alcohol lamp and other essential safety procedures.
  • Bacteria should be operated in laminar flow hood to secure isolated environment
  • Wastes containing living organisms should be autoclaved before throwing away and sent to certain institution for disposal
  • Glass apparatus should be disinfected regularly
  • Rubber gloves, lab coats, long pants and shoes should be equipped to cover the entire skin when conducting experiment
  • Water, fire, electricity and air conditioner should be examined whenever an experiment is suspended
  • The laboratory should be cleaned at least once a week

Safety tutoring

Before our team members commenced on the experiment, we have been trained with safety issues systematically.For example, we have been taught how to use extinguishers and fire blankets for in case, also we ‘ve learned the rules of waste cleaning and made a schedule for it. We give sincere thanks to Professor Ren from Shandong University for offering the training session.

Safety concerns of our project

Our project aims to increase the sensitivity and specificity on the testing for HCV in blood. In the future, we will request a department which is qualified with Biosafety Level 3 to test our system using HCV infected serum. If it is applicable after further evaluation, there will be a significant decrease in the possibility that patients would received infected blood. Thus, our project will add insurance to public hygiene with higher efficiency and lower cost on HCV detection.