Difference between revisions of "Team:JNFLS/Safety"
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<h1> Safety </h1> | <h1> Safety </h1> | ||
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<p>Biosafety is the prevention of exposure to biohazards or pathogen dissemination, concerning on the issues of ecology and hygiene. Human errors, unqualified instruments, poor experimental techniques would all possibly contribute to deficient safety insurance. Typical topics in biosafefy involve the regular conduction of examinations on laboratory settings and machines, a set of strict laboratory guidelines to obey, etc. Working with synthetic biology experiment, especially high-security apparatus and facilities are required to prevent harmful chemicals or engineered organisms from being released accidentally. </p> | <p>Biosafety is the prevention of exposure to biohazards or pathogen dissemination, concerning on the issues of ecology and hygiene. Human errors, unqualified instruments, poor experimental techniques would all possibly contribute to deficient safety insurance. Typical topics in biosafefy involve the regular conduction of examinations on laboratory settings and machines, a set of strict laboratory guidelines to obey, etc. Working with synthetic biology experiment, especially high-security apparatus and facilities are required to prevent harmful chemicals or engineered organisms from being released accidentally. </p> | ||
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<h2>Following promise</h2> | <h2>Following promise</h2> | ||
<h3>Biosafety Level of Our Laboratory</h3> | <h3>Biosafety Level of Our Laboratory</h3> | ||
| − | <p>With the guidance from iGEM competition and locally safety regulation, and in order to keep our members from bio risks and correspond to the regulations above, our laboratory is ranked as BSL-1. Biosafety Level 2 laboratory generally deal with applicable microorganisms without harmful pathogenic potential on healthy adults. Additionally, all of the parts and species we utilized, which are listed in the chart below, are environmental friendly, not potentially harmful or pathogenic to healthy adult individuals. </p> | + | <p>With the guidance from iGEM competition and locally safety regulation, and in order to keep our members from bio risks and correspond to the regulations above, our laboratory is ranked as BSL-1. Biosafety Level 2 laboratory generally deal with applicable microorganisms without harmful pathogenic potential on healthy adults. Additionally, all of the parts and species we utilized, which are listed in the chart below, are environmental friendly, not potentially harmful or pathogenic to healthy adult individuals.Before entering the labortary, everyone can notoce the reminders as follows: </p> |
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| + | <img src="https://static.igem.org/mediawiki/2018/8/88/T--JNFLS--S1.jpg"style="width:35%"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/4/44/T--JNFLS--SA2.jpg"style="width:40%"> | ||
<h3>Lab Equipment</h3> | <h3>Lab Equipment</h3> | ||
| − | <p>Our lab is equipped with advanced instruments to ensure safety, including laminar flow hood, autoclaves, and personal protective equipment.</p> | + | <p>Our lab is equipped with advanced instruments to ensure safety, including laminar flow hood, autoclaves, and personal protective equipment. We also set a trash can especially for polluted wastes.And we have a medical box contains some available medical items at any time.</p> |
| + | <img src="https://static.igem.org/mediawiki/2018/a/a1/T--JNFLS--SA3.jpg"style="width:40%"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/1/1c/T--JNFLS--SA4.jpg"style="width:40%"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/4/4c/T--JNFLS--S8.jpg"style="width:44%"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/1/1d/T--JNFLS--S4.jpg"style="width:40%"> | ||
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<h3>Experiment operation</h3> | <h3>Experiment operation</h3> | ||
<p>During the entire experiment, each member strictly followed the criteria as presented:</p> | <p>During the entire experiment, each member strictly followed the criteria as presented:</p> | ||
<ul> | <ul> | ||
| − | <li>Fire, electric heaters and microwaves should not be left unheeded</li> | + | <li>Fire, electric heaters and microwaves should not be left unheeded.Our lab is also well equipped with fire hydrant, fire extinguishers and fire blankets to make sure the fire could be extinguished within a short period of time. Our safety instructor has taught everyone how to use these extinguishing equipment. We have also studied the using method of alcohol lamp and other essential safety procedures. </li> |
| + | <img src="https://static.igem.org/mediawiki/2018/7/72/T--JNFLS--S5.jpg"style="width:45%"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/b/b0/T--JNFLS--S3.jpg"style="width:45%"> | ||
<li>Bacteria should be operated in laminar flow hood to secure isolated environment</li> | <li>Bacteria should be operated in laminar flow hood to secure isolated environment</li> | ||
<li>Wastes containing living organisms should be autoclaved before throwing away and sent to certain institution for disposal</li> | <li>Wastes containing living organisms should be autoclaved before throwing away and sent to certain institution for disposal</li> | ||
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</ul> | </ul> | ||
<h3>Safety tutoring</h3> | <h3>Safety tutoring</h3> | ||
| − | <p>Before our team members commenced on the experiment, we have been trained with safety issues systematically. We give sincere thanks to Professor Ren from Shandong University for offering the training session.</p> | + | <p>Before our team members commenced on the experiment, we have been trained with safety issues systematically.For example, we have been taught how to use extinguishers and fire blankets for in case, also we ‘ve learned the rules of waste cleaning and made a schedule for it. We give sincere thanks to Professor Ren from Shandong University for offering the training session.</p> |
| + | <img src="https://static.igem.org/mediawiki/2018/8/81/T--JNFLS--SA5.jpg"style="width:45%"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/9/98/T--JNFLS--oo.jpg"style="width:45%"> | ||
<h3>Safety concerns of our project</h3> | <h3>Safety concerns of our project</h3> | ||
| − | <p>Our project aims to increase the sensitivity and specificity on the testing | + | <p>Our project aims to increase the sensitivity and specificity on the testing for HCV in blood. In the future, we will request a department which is qualified with Biosafety Level 3 to test our system using HCV infected serum. If it is applicable after further evaluation, there will be a significant decrease in the possibility that patients would received infected blood. Thus, our project will add insurance to public hygiene with higher efficiency and lower cost on HCV detection. |
| + | </p> | ||
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Latest revision as of 13:27, 15 October 2018
Safety
Biosafety is the prevention of exposure to biohazards or pathogen dissemination, concerning on the issues of ecology and hygiene. Human errors, unqualified instruments, poor experimental techniques would all possibly contribute to deficient safety insurance. Typical topics in biosafefy involve the regular conduction of examinations on laboratory settings and machines, a set of strict laboratory guidelines to obey, etc. Working with synthetic biology experiment, especially high-security apparatus and facilities are required to prevent harmful chemicals or engineered organisms from being released accidentally.
Following promise
Biosafety Level of Our Laboratory
With the guidance from iGEM competition and locally safety regulation, and in order to keep our members from bio risks and correspond to the regulations above, our laboratory is ranked as BSL-1. Biosafety Level 2 laboratory generally deal with applicable microorganisms without harmful pathogenic potential on healthy adults. Additionally, all of the parts and species we utilized, which are listed in the chart below, are environmental friendly, not potentially harmful or pathogenic to healthy adult individuals.Before entering the labortary, everyone can notoce the reminders as follows:
Lab Equipment
Our lab is equipped with advanced instruments to ensure safety, including laminar flow hood, autoclaves, and personal protective equipment. We also set a trash can especially for polluted wastes.And we have a medical box contains some available medical items at any time.
Experiment operation
During the entire experiment, each member strictly followed the criteria as presented:
- Fire, electric heaters and microwaves should not be left unheeded.Our lab is also well equipped with fire hydrant, fire extinguishers and fire blankets to make sure the fire could be extinguished within a short period of time. Our safety instructor has taught everyone how to use these extinguishing equipment. We have also studied the using method of alcohol lamp and other essential safety procedures.
- Bacteria should be operated in laminar flow hood to secure isolated environment
- Wastes containing living organisms should be autoclaved before throwing away and sent to certain institution for disposal
- Glass apparatus should be disinfected regularly
- Rubber gloves, lab coats, long pants and shoes should be equipped to cover the entire skin when conducting experiment
- Water, fire, electricity and air conditioner should be examined whenever an experiment is suspended
- The laboratory should be cleaned at least once a week
Safety tutoring
Before our team members commenced on the experiment, we have been trained with safety issues systematically.For example, we have been taught how to use extinguishers and fire blankets for in case, also we ‘ve learned the rules of waste cleaning and made a schedule for it. We give sincere thanks to Professor Ren from Shandong University for offering the training session.
Safety concerns of our project
Our project aims to increase the sensitivity and specificity on the testing for HCV in blood. In the future, we will request a department which is qualified with Biosafety Level 3 to test our system using HCV infected serum. If it is applicable after further evaluation, there will be a significant decrease in the possibility that patients would received infected blood. Thus, our project will add insurance to public hygiene with higher efficiency and lower cost on HCV detection.