Difference between revisions of "Team:Munich/chibiobrick1.html"
Franziwinzig (Talk | contribs) |
Franziwinzig (Talk | contribs) |
||
| Line 9: | Line 9: | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Dominic</td> | + | <td>Dominic Schwarz</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 25: | Line 25: | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Dominic</td> | + | <td>Dominic Schwarz</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 58: | Line 58: | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Dominic</td> | + | <td>Dominic Schwarz</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 14:02, 15 October 2018
Forming oligodimers from single-strand DNA chi6 sequences
2018/06/01| Participants: | Dominic Schwarz |
| Protocol: | Oligodimerization |
Assembling pSB1C3_Chi6 with A3 Assembly
2018/06/04| Participants: | Dominic Schwarz |
| Protocol: | Restrition digest, Ligation, Chemical transformation, Mini prep, Sequencing |
| Notes: | digest with XbaI, SpeI transformation of Dh5a |
| Results: | no sequence obtained for the given primers. The samples must be contaminated. We decided to redo the experiment. |
Redo: Assembling pSB1C3_Chi6 with A3 Assembly
2018/06/06 - 2018/06/26| Participants: | Dominic Schwarz |
| Protocol: | Restrition digest, PCR purification, Ligation, Chemical Transformation, PCR, Agarose gel |
| Notes: | digest with XbaI, SpeI transformation of Dh5a |
| Results: | No bands on the Gel. Colonies on the plate resulted from contamination. |