Difference between revisions of "Team:Munich/chiversions.html"
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| Line 14: | Line 14: | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td>PCR, | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, | ||
| + | <a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel Purification</a>, | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 39: | Line 43: | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td>PCR, Agarose gel, gel purification, gibson assembly</td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, | ||
| + | <a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel purification</a>, | ||
| + | <a href="https://international.neb.com/protocols/2012/12/11/gibson-assembly-protocol-e5510" target="_blank">Gibson Assembly</a>, | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 63: | Line 72: | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td>PCR, | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, | ||
| + | <a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel extraction</a>, | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 87: | Line 100: | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td>PCR</td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 14:25, 15 October 2018
PCR amplify linear mtq2
2018/05/28 - 2018/05/29| Participants: | Dominic Schwarz |
| Protocol: | PCR, Agarose gel, Gel Purification, |
| Notes: | VR & VF2; TA: 66° ET: 50s; Template: linear mtq2_bivtat from Lukas Aufinger (Supervisor) |
| Results: | worked (PIC) |
Assembling Chi6(double) and Chi6-Mtq2-Chi6 by gibson assembly
2018/06/04 - 2018/06/08| Participants: | Dominic Schwarz |
| Protocol: | PCR, Agarose gel, Gel purification, Gibson Assembly, |
| Notes: | Fragments were amplified, then purified and gibson assembly was performed |
| Results: | No bands were visible on the gel after gibson assembly, suggesting that fragments were not amplified correctly before. additionally, we decided to do overlap pcr instead of gibson assembly. |
Assembling Chi6(double) and Chi6-Mtq2-Chi6 by PCR
2018/06/11 - 2018/06/14| Participants: | Dominic Schwarz |
| Protocol: | PCR, Agarose gel, Gel extraction, |
| Notes: | Fragments were amplified, then purified and overlap PCR was performed. |
| Results: | The Agarose gel after overlap PCR was produced incorrectly, therefore we lost the samples. |
PCR to amplify Mtq2
2018/06/12| Participants: | Dominic Schwarz |
| Protocol: | PCR, |
| Notes: | Primers: VF2, VR |
| Results: |