Difference between revisions of "Team:Munich/chiversions.html"

Revision as of 14:26, 15 October 2018

PCR amplify linear mtq2

2018/05/28 - 2018/05/29

Participants:	Dominic Schwarz
Protocol:	PCR, Agarose gel, Gel Purification
Notes:	VR & VF2; TA: 66° ET: 50s; Template: linear mtq2_bivtat from Lukas Aufinger (Supervisor)
Results:	worked (PIC)

Assembling Chi6(double) and Chi6-Mtq2-Chi6 by gibson assembly

2018/06/04 - 2018/06/08

Participants:	Dominic Schwarz
Protocol:	PCR, Agarose gel, Gel purification, Gibson Assembly
Notes:	Fragments were amplified, then purified and gibson assembly was performed
Results:	No bands were visible on the gel after gibson assembly, suggesting that fragments were not amplified correctly before. additionally, we decided to do overlap pcr instead of gibson assembly.

Assembling Chi6(double) and Chi6-Mtq2-Chi6 by PCR

2018/06/11 - 2018/06/14

Participants:	Dominic Schwarz
Protocol:	PCR, Agarose gel, Gel extraction
Notes:	Fragments were amplified, then purified and overlap PCR was performed.
Results:	The Agarose gel after overlap PCR was produced incorrectly, therefore we lost the samples.

PCR to amplify Mtq2

2018/06/12

Participants:	Dominic Schwarz
Protocol:	PCR
Notes:	Primers: VF2, VR
Results:

@@ Line 17: / Line 17: @@
 <a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>,
 <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>,
-<a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel Purification</a>,
+<a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel Purification</a>
 </td>
      </tr>
@@ Line 47: / Line 47: @@
 <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>,
 <a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel purification</a>,
-<a href="https://international.neb.com/protocols/2012/12/11/gibson-assembly-protocol-e5510" target="_blank">Gibson Assembly</a>,
+<a href="https://international.neb.com/protocols/2012/12/11/gibson-assembly-protocol-e5510" target="_blank">Gibson Assembly</a>
 </td>
      </tr>
@@ Line 75: / Line 75: @@
 <a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>,
 <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>,
-<a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel extraction</a>,
+<a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel extraction</a>
 </td>
      </tr>
@@ Line 101: / Line 101: @@
        <td>Protocol:</td>
        <td>
-<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>,
+<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>
 </td>
      </tr>