Difference between revisions of "Team:Munich/deletioncasette.html"
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| Line 8: | Line 8: | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Dominic</td> | + | <td>Dominic Schwarz</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank">Electrocompetent transformation</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 32: | Line 34: | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td> | + | <td>Enikö Baligács</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td>PCR, | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, | ||
| + | <a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel extraction</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 20:08, 15 October 2018
Transforming E. Coli DH5a to amplify pKD3 for pRED/ET engineering
2018/05/28| Participants: | Dominic Schwarz |
| Protocol: | Electrocompetent transformation |
| Notes: | we also inoculated LB media blindly. |
| Results: | no colonies, no growth; We decided to use a resistance cassette from pSB1C3 without FRT sites |
Amplifying a selection cassette from pSB1C3
2018/05/29| Participants: | Enikö Baligács |
| Protocol: | PCR, Agarose gel, Gel extraction |
| Notes: | Primer with 50bp homology regions ????, TA: 67°C, Elongation: 45s |
| Results: | PIC. we thought the band might be what we wanted but because of how few DNA the extraction yielded, these samples were not used in further experiments. |