Difference between revisions of "Team:Munich/deletioncasette2.html"
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<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Dominic</td> | + | <td>Dominic Schwarz</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td>PCR, Agarose | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, | ||
| + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, | ||
| + | <a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel extraction</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 20:37, 15 October 2018
Creating a selection cassette from pSB1C3
2018/08/28| Participants: | Dominic Schwarz |
| Protocol: | PCR, Agarose gel, Gel extraction |
| Notes: | Knockouts of both msb-B, RecBCD Primer with 50bp homology regions, Ta: 62°C, Elongation: 45s Expected: 1,1kb |
| Results: | 2 PCR reactions worked. One reaction for a resistance cassette flanked with FRT sites didnt work. Elena (Advisor) suggested that the template might be incorrect. |
