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Revision as of 21:19, 15 October 2018

Transforming E. Coli DH5a to amplify plasmids for pRED/ET engineering

2018/08/28
Participants: Dominic Schwarz
Protocol: ELectrocompetent transformation
Notes: inoculate pRED/ET at 30°C because of temperature sensitive promoter on the plasmid.
Results: ~200 colonies