Difference between revisions of "Team:Munich/growth.html"
ArianeKrus (Talk | contribs) |
|||
| Line 8: | Line 8: | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Katja</td> | + | <td>Katja Neishsalo</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 31: | Line 31: | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Katja</td> | + | <td>Katja Neishsalo</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://www.neb.com/protocols/2012/06/21/making-your-own-electrocompetent-cells" target="_blank">Preparation electrocompetent cells</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 49: | Line 51: | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Katja</td> | + | <td>Katja Neishsalo</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td> |
| + | <a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank">Electrocompetent transformation</a> | ||
| + | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 67: | Line 71: | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Katja</td> | + | <td>Katja Neishsalo</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td> | + | <td>Midiprep</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 80: | Line 84: | ||
<tr> | <tr> | ||
<td>Participants:</td> | <td>Participants:</td> | ||
| − | <td>Katja</td> | + | <td>Katja Neishsalo</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 22:20, 15 October 2018
Growth of msbB and RecBCD-Nuklease deficient E.Coli mutant strains
2018/08/28| Participants: | Katja Neishsalo |
| Protocol: | Measuring optical density |
| Notes: | |
| Results: | PIC |
preparing electro-competent cells of WT RecBCD und dRecB
2018/08/31| Participants: | Katja Neishsalo |
| Protocol: | Preparation electrocompetent cells |
| Results: | PICcells are still viable after preparation, dRecB grows significantly slower than others |
Transforming E. Coli dRecB with pSB1C3_RecBCD(WT)
2018/09/03| Participants: | Katja Neishsalo |
| Protocol: | Electrocompetent transformation |
| Results: | colonies |
DNA midi prep of RecBCD(WT) plasmid from Dh5a
2018/09/03| Participants: | Katja Neishsalo |
| Protocol: | Midiprep |
Growth of RecB, RecD, RecBCD, msb�-B deficient E.Coli strains
2018/09/14| Participants: | Katja Neishsalo |
| Protocol: | Measuring optical density |
| Notes: | |
| Results: | PIC |