Revision as of 21:30, 25 June 2018

Notebook

Week 1 (June 4-8): •Made competent cells
Week 2 (June 11-15): •Testing competent cells using iGem DNA transformation samples.; • Tested to see if they grew better on a freezer block versus an ice bath.; • Ran colony PCR and plated bacteria on antimicrobial plate.; • cPCR, chloramphenicol, and culturing protocols, made agarose gel; • gel electrophoresis for cPCR, nucleic acid purification plasmid
Week 3 (June 18-22): •Made chloramphenicol LB plates; •Restriction enzyme digest of iGem plasmid PSB1C3 using EcoRI and PstI; •Made digest master mix; ran an ezyme digest of iGem plasmids PSB1A3 and PSB1K3 Performed ligation of; PSB1A3 and PSB1K3; transformed PSB1A3 in competent cells on ampicillin plate; •Created a 50mg/mL kanamycin solution and then alloquoted it to about 80 1.5mL tubes.; •Inoculated LB with ampicillin colonies (with insert).; •Streaked ampicillin (IA3) colony onto new plates.; •Conducted cPCR for IA3 colonies with insert.
Week 4 (June 25-29): •Conducted cPCR for PSB1K3.; •Ran 1% agarose gel of PSBIA3 with the insert (colonies that were re-streaked).; •Ran 1% agarose gel of PSBK3 with insert.

@@ Line 36: / Line 36: @@
 <dd>•Conducted cPCR for PSB1K3.</dd>
 <dd>•Ran 1% agarose gel of PSBIA3 with the insert (colonies that were re-streaked).</dd>
+<dd>•Ran 1% agarose gel of PSBK3 with insert.</dd>