Difference between revisions of "Team:NUS Singapore-Sci/Parts"
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<th>Component</th> | <th>Component</th> | ||
<th>Description</th> | <th>Description</th> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>rAPOBEC1</td> | <td>rAPOBEC1</td> | ||
<td>Rat APOBEC1. Used to perform the C-to-U conversion.</td> | <td>Rat APOBEC1. Used to perform the C-to-U conversion.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>dPspCas13b</td> | <td>dPspCas13b</td> | ||
<td>"Dead" Cas13 from <i>Prevotella sp.</i>. Used to target specific mRNA strands.</td> | <td>"Dead" Cas13 from <i>Prevotella sp.</i>. Used to target specific mRNA strands.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>PspCas13b</td> | <td>PspCas13b</td> | ||
<td>Cas13 from <i>Prevotella sp.</i>. Used to target and cut specific mRNA strands.</td> | <td>Cas13 from <i>Prevotella sp.</i>. Used to target and cut specific mRNA strands.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>eGFP</td> | <td>eGFP</td> | ||
<td>Consistutively expressed GFP reporter. Used in the Reporter system.</td> | <td>Consistutively expressed GFP reporter. Used in the Reporter system.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>mCherry</td> | <td>mCherry</td> | ||
<td>Red flurophore protein. Used in the Reporter system.</td> | <td>Red flurophore protein. Used in the Reporter system.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>T2A</td> | <td>T2A</td> | ||
<td>Self cleavage peptide. Used in the Reporter system.</td> | <td>Self cleavage peptide. Used in the Reporter system.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>GLB1</td> | <td>GLB1</td> | ||
<td>Human lysosomal acid beta galactosidase. Used in the GLB reporter system.</td> | <td>Human lysosomal acid beta galactosidase. Used in the GLB reporter system.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>eGFP mutant reporter</td> | <td>eGFP mutant reporter</td> | ||
<td>eGFP with a mutated start codon (ATG -> ACG).</td> | <td>eGFP with a mutated start codon (ATG -> ACG).</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>GLB1 CCG mutant</td> | <td>GLB1 CCG mutant</td> | ||
<td>Non-functional beta galactosidase with mutation of I389 to P389.</td> | <td>Non-functional beta galactosidase with mutation of I389 to P389.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>GLB1 TCT mutant</td> | <td>GLB1 TCT mutant</td> | ||
<td>Non-functional beta galactosidase with mutation of F107 to L107.</td> | <td>Non-functional beta galactosidase with mutation of F107 to L107.</td> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 05:45, 16 October 2018
Parts
Basic Parts
| Part Name | Component | Description |
|---|---|---|
| BBa_K2807000 | rAPOBEC1 | Rat APOBEC1. Used to perform the C-to-U conversion. |
| BBa_K2807001 | dPspCas13b | "Dead" Cas13 from Prevotella sp.. Used to target specific mRNA strands. |
| BBa_K2807002 | PspCas13b | Cas13 from Prevotella sp.. Used to target and cut specific mRNA strands. |
| BBa_K2807005 | eGFP | Consistutively expressed GFP reporter. Used in the Reporter system. |
| BBa_K2807006 | mCherry | Red flurophore protein. Used in the Reporter system. |
| BBa_K2807007 | T2A | Self cleavage peptide. Used in the Reporter system. |
| BBa_K2807008 | GLB1 | Human lysosomal acid beta galactosidase. Used in the GLB reporter system. |
| BBa_K2807009 | eGFP mutant reporter | eGFP with a mutated start codon (ATG -> ACG). |
| BBa_K2807010 | GLB1 CCG mutant | Non-functional beta galactosidase with mutation of I389 to P389. |
| BBa_K2807011 | GLB1 TCT mutant | Non-functional beta galactosidase with mutation of F107 to L107. |
Composite Parts
| Part Name | Component | Description | Submission |
|---|---|---|---|
| BBa_K2807003 | rAPOBEC1-XTEN-dPspCas13b | The completed Editor system. | N |
| BBa_K2807012 | eGFP-T2A-mCherry | The completed Dual-Reporter system. | Y |
| BBa_K2807013 | eGFP-T2A-mCherry mutant | The completed Dual-Reporter system, mutated for BioBric competency. | Y |
| BBa_K2807014 | eGFP-GLB1 WT | The completed GLB-Reporter system. | Y |
| BBa_K2807015 | eGFP-GLB1 CCG mutant | Y | |
| BBa_K2807016 | eGFP-GLB1 TCT mutant | Y |