Difference between revisions of "Team:HUST-China/Notebook"

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<h1>Notebook</h1>
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/project/background">Background</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Description">Description</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Experiments">Experiments</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/wetlab/protocols">Protocols</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/InterLab">Interlab</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Improve">Improve</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Notebook">Notebook</a></li>
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                          <a href="#" data-toggle="dropdown" class="dropdown-toggle waves-effect waves-dark">Model<b class="caret"></b></a>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Modeling overview">Modeling overview</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Comparison between PSB">Comparison between PSB</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Sort of three genes">Sort of three genes</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Parts Overview">Parts Overview</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Composite part">Composite part</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Part collection">Part collection</a></li>
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                          <a href="#" data-toggle="dropdown" class="dropdown-toggle waves-effect waves-dark">HP<b class="caret"></b></a>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Education Engagement">Education&Engagement</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Team Members">Team Members</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Collaborations">Collaborations</a></li>
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                              <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Attributions">Attributions</a></li>
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                    </ul>
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        <h2 class="pageTitle">NoteBooks</h2>
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  </section>
  
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<h3>What should this page have?</h3>
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  <div class="container">
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<ul>
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        <div class="row">  
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<li>Chronological notes of what your team is doing.</li>
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            <div class="col-md-12">  
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<li> Brief descriptions of daily important events.</li>
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              <!-- Accordion starts -->
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<li>Pictures of your progress. </li>
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              <ul class="collapsible" id="accordionSection" data-collapsible="accordion">
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<li>Mention who participated in what task.</li>
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                <li>
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</ul>
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                  <div class="collapsible-header HillSide"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>April 1-30</div>
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                  <div class="collapsible-body"><p>Ziyang Xiao, Junhao Xiong, Jingyu Ren, Yan chen, Qian Li<br>Pre-experiment of electrogenesis: tested and optimized the electrogenesis condition for MFC (Microbial Fuel Cell)</p></div>
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                </li>
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                <li>
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                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>May 1-31</div>
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                  <div class="collapsible-body"><p>Yiyan Yu, Ziyi Li, Junhao Xiong<br>
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                    Basic design of molecular experiments: design sequences, primers and the construction of Shewanella
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                  </p></div>
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                </li>
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                <li>
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                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>July 1-7</div>
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                  <div class="collapsible-body"><p>Junhao Xiong, Ziqi Yin, Jiayi Liu<br>
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                    Preparation for following experiments including preparing LB culture medium, antibiotics and optimizing culture condition of Shewanella oneidensis MR-1.<br>
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                    Bo Peng<br>
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                    Construction of pSB1C3-RBS-mleS
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                    </p>
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                    <div class="col-md-4 col-md-offset-1">
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                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/7/77/T--HUST-China--2018-notebook01.png">
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                    </div>
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                    <div class="col-md-12">
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                      <p>Ziyang Xiao, Haotian Ren, Yan Chen, Hao Qiu<br>
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                      Cyanobacteria Group took the responsibility of doing the Interlab. Preparation for the experiment.
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                      </p>
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                    </div>
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                  </div>
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                </li>
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                <li>
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                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>July 8-21</div>
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                  <div class="collapsible-body"><p>Junhao Xiong, Ziqi Yin, Jiayi Liu<br>
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                  Construction of pYYDT-pflB: PCR, double enzyme digestion (with NdeI & SalI), ligation, transformation (into E.coli TOP10).
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                  </p>
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                    <div class="col-md-6 col-md-offset-1">
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                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/1/12/T--HUST-China--2018-notebook02.png">
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                    </div>
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                    <div class="col-md-12">
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                      <p>Qian Li<br>
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                        Construction of pSB1C3-RBS-lldp-RBS-ldhA-TT
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                      </p>
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                    </div>
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                    <div class="col-md-4 col-md-offset-1">
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                      <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/2/2b/T--HUST-China--2018-notebook03.png">
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                    </div>
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                    <div class="col-md-12">
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                      <p><span class="red-content">Ziyang Xiao, Haotian Ren, Yan Chen, Hao Qiu</span><br>
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                        Finishing the cell cultures cultivation and using some machines to measure some related data.
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                      </p>
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                    </div>
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                  </div>
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                </li>
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                <li>
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                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>Human Practices</div>
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                  <div class="collapsible-body"><p>The main organizers of “HUST-Cheering!” intercommunions are Shengde Deng, Jingyu Ren, Zixin Yang and Ying Xu. All members of HUST-China team helped in holding this regional jamboree. Shengde Deng, as the leader of the Human Practice, coordinated all the work, contacted company for visits and other iGEM teams for collaboration and arranged for the internal players to be responsible for the corresponding affairs. Jingyu Ren, was responsible for visit to two enterprises, Zixin Yang was responsible for the survey and editing for the tweet while Xing Xu was responsible for participating for and co-organizing the CCIC. In addition, Shengde Deng ,Zixin Xu and Xing Xu also hosted the school summer camp in order to introduce synthetic Biology and Bio battery to high school students.</p></div>
 +
                </li>
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                <li>
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                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>Advisors</div>
 +
                  <div class="collapsible-body">
 +
                    <p>Professor Yunjun Yan</br>
 +
                    Professor Kang Ning</br>
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                    Dr. Yi Zhan</br>
 +
                    Thanks for their generous support and careful guidance.</p>
 +
                  </div>
 +
                </li>
 +
                <li>
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                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>Special Thanks</div>
 +
                  <div class="collapsible-body">
 +
                    <p>Especially thanks Team USTC for providing pYYDT vector and E.coli WM3064 .</br>
 +
                    Especially thanks Professor Hao Song and Dr. Feng Li for giving advice for our design.</br>
 +
                    Especially thanks Professor Chieh-Chen Huang, Dr. Shou-Chen Lo and Team NCHU_Taiwan for providing pMG105 vector and pMG105-PpckA vector.</br>
 +
                    Thanks Dr Wu Liu and Dr Qinghua Zhou for their direct guidance in experiment.</br>
 +
                    Thanks</p>
 +
                  </div>
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                </li>
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                <li>
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                  <div class="collapsible-header"><i><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/89/T--HUST-China--2018-coin04.png"></i>Acknowledgments</div>
 +
                  <div class="collapsible-body">
 +
                      <p>This project was supported by Huazhong University of Science and Technology, College of Life Science and Technology and Qiming College of HUST.</br>
 +
                      Free software is offered by Mathworks. </br>
 +
                      DNA synthesis services are supported by IDT for free and by Genscript.</br>
 +
                      Primer synthesis and sequencing services are provided by Genscript, TIANYI HUIYUAN and TsingKe.</p>
 +
                  </div>
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                </li>
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</section>
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                College of Life Science & Technology<br>
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                Huazhong University of Science and Technology<br>
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                Copyright &copy;2018 Huazhong University of Science & Technology. Produced By </span><a href="https://2018.igem.org/Team:HUST-China" target="_blank" style="color:white;">HUST-China</a>
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Revision as of 08:48, 16 October 2018

iGEM 2018 HUST-China

NoteBooks

  • April 1-30

    Ziyang Xiao, Junhao Xiong, Jingyu Ren, Yan chen, Qian Li
    Pre-experiment of electrogenesis: tested and optimized the electrogenesis condition for MFC (Microbial Fuel Cell)

  • May 1-31

    Yiyan Yu, Ziyi Li, Junhao Xiong
    Basic design of molecular experiments: design sequences, primers and the construction of Shewanella

  • July 1-7

    Junhao Xiong, Ziqi Yin, Jiayi Liu
    Preparation for following experiments including preparing LB culture medium, antibiotics and optimizing culture condition of Shewanella oneidensis MR-1.
    Bo Peng
    Construction of pSB1C3-RBS-mleS

    Ziyang Xiao, Haotian Ren, Yan Chen, Hao Qiu
    Cyanobacteria Group took the responsibility of doing the Interlab. Preparation for the experiment.

  • July 8-21

    Junhao Xiong, Ziqi Yin, Jiayi Liu
    Construction of pYYDT-pflB: PCR, double enzyme digestion (with NdeI & SalI), ligation, transformation (into E.coli TOP10).

    Qian Li
    Construction of pSB1C3-RBS-lldp-RBS-ldhA-TT

    Ziyang Xiao, Haotian Ren, Yan Chen, Hao Qiu
    Finishing the cell cultures cultivation and using some machines to measure some related data.

  • Human Practices

    The main organizers of “HUST-Cheering!” intercommunions are Shengde Deng, Jingyu Ren, Zixin Yang and Ying Xu. All members of HUST-China team helped in holding this regional jamboree. Shengde Deng, as the leader of the Human Practice, coordinated all the work, contacted company for visits and other iGEM teams for collaboration and arranged for the internal players to be responsible for the corresponding affairs. Jingyu Ren, was responsible for visit to two enterprises, Zixin Yang was responsible for the survey and editing for the tweet while Xing Xu was responsible for participating for and co-organizing the CCIC. In addition, Shengde Deng ,Zixin Xu and Xing Xu also hosted the school summer camp in order to introduce synthetic Biology and Bio battery to high school students.

  • Advisors

    Professor Yunjun Yan
    Professor Kang Ning
    Dr. Yi Zhan
    Thanks for their generous support and careful guidance.

  • Special Thanks

    Especially thanks Team USTC for providing pYYDT vector and E.coli WM3064 .
    Especially thanks Professor Hao Song and Dr. Feng Li for giving advice for our design.
    Especially thanks Professor Chieh-Chen Huang, Dr. Shou-Chen Lo and Team NCHU_Taiwan for providing pMG105 vector and pMG105-PpckA vector.
    Thanks Dr Wu Liu and Dr Qinghua Zhou for their direct guidance in experiment.
    Thanks

  • Acknowledgments

    This project was supported by Huazhong University of Science and Technology, College of Life Science and Technology and Qiming College of HUST.
    Free software is offered by Mathworks.
    DNA synthesis services are supported by IDT for free and by Genscript.
    Primer synthesis and sequencing services are provided by Genscript, TIANYI HUIYUAN and TsingKe.

College of Life Science & Technology
Huazhong University of Science and Technology
Add: 1037 Luoyu Road, Wuhan, Hubei, China; P.C: 430074
Copyright ©2018 Huazhong University of Science & Technology. Produced By HUST-China