Difference between revisions of "Template:Groningen/Design"
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<li id="timeline-scroll" class="scrollspy"> | <li id="timeline-scroll" class="scrollspy"> | ||
<div class="collapsible-header"> | <div class="collapsible-header"> | ||
| − | Design | + | Design (click me!) |
</div> | </div> | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
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<div id="cellulose_input" class="modal" style="z-index: 1003;"> | <div id="cellulose_input" class="modal" style="z-index: 1003;"> | ||
<div class="modal-content"> | <div class="modal-content"> | ||
| − | + | <h4>Cellulose input</h4> | |
| − | <h4>Cellulose input</h4> | + | |
<ul class="collapsible" data-collapsible="expandable"> | <ul class="collapsible" data-collapsible="expandable"> | ||
<li><div class="collapsible-header">1.1 What is a good cellulose source for our product?</div> | <li><div class="collapsible-header">1.1 What is a good cellulose source for our product?</div> | ||
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<li><div class="collapsible-header">1.2 How do we preprocess the cellulose-containing biomass for optimal use?</div> | <li><div class="collapsible-header">1.2 How do we preprocess the cellulose-containing biomass for optimal use?</div> | ||
<div class="collapsible-body"></div></li> | <div class="collapsible-body"></div></li> | ||
| − | </ul> | + | </ul> |
| + | </div> | ||
</div> | </div> | ||
<div id="towards_glucose" class="modal" style="z-index: 1003;"> | <div id="towards_glucose" class="modal" style="z-index: 1003;"> | ||
<div class="modal-content"> | <div class="modal-content"> | ||
<h4>Cellulose towards glucose</h4> | <h4>Cellulose towards glucose</h4> | ||
| − | < | + | <ul class="collapsible" data-collapsible="expandable"> |
| − | + | <li><div class="collapsible-header">2.1 Which enzymes do we need to degrade cellulose effectively?</div> | |
| − | </ | + | <div class="collapsible-body"></div></li> |
| + | <li><div class="collapsible-header">2.2 What is a mini-cellulosome?</div> | ||
| + | <div class="collapsible-body"></div></li> | ||
| + | </ul> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="modal-content"> | <div class="modal-content"> | ||
<h4>Glucose towards StyGreen</h4> | <h4>Glucose towards StyGreen</h4> | ||
| − | < | + | <ul class="collapsible" data-collapsible="expandable"> |
| − | + | <li><div class="collapsible-header">3.1 How to convert glucose into styrene?</div> | |
| − | + | <div class="collapsible-body"></div></li> | |
| + | |||
| + | </ul> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="modal-content"> | <div class="modal-content"> | ||
<h4>Host strain</h4> | <h4>Host strain</h4> | ||
| − | < | + | <ul class="collapsible" data-collapsible="expandable"> |
| − | + | <li><div class="collapsible-header">4.1 Why Saccharomyces cerevisiae?</div> | |
| − | </ | + | <div class="collapsible-body"></div></li> |
| + | <li><div class="collapsible-header">4.2 How do we genetically modify S. cerevisiae?</div> | ||
| + | <div class="collapsible-body"></div></li> | ||
| + | </ul> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="modal-content"> | <div class="modal-content"> | ||
<h4>Further improving the process towards glucose</h4> | <h4>Further improving the process towards glucose</h4> | ||
| − | < | + | <ul class="collapsible" data-collapsible="expandable"> |
| − | + | <li><div class="collapsible-header">5.1 How to adapt the expression system for sustained growth on cellulose?</div> | |
| − | + | <div class="collapsible-body"></div></li> | |
| + | |||
| + | </ul> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="modal-content"> | <div class="modal-content"> | ||
<h4>Further improving the process towards StyGreen</h4> | <h4>Further improving the process towards StyGreen</h4> | ||
| − | < | + | <ul class="collapsible" data-collapsible="expandable"> |
| − | + | <li><div class="collapsible-header">6.1 How to improve the styrene yield?</div> | |
| − | + | <div class="collapsible-body"></div></li> | |
| + | |||
| + | </ul> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="modal-content"> | <div class="modal-content"> | ||
<h4>Final strain optimization</h4> | <h4>Final strain optimization</h4> | ||
| − | < | + | <ul class="collapsible" data-collapsible="expandable"> |
| − | + | <li><div class="collapsible-header">7.1 How to further improve our StyGreen producing strain?</div> | |
| − | + | <div class="collapsible-body"></div></li> | |
| + | <li><div class="collapsible-header">7.2 How do we combat styrene toxicity?</div> | ||
| + | <div class="collapsible-body"></div></li> | ||
| + | </ul> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="modal-content"> | <div class="modal-content"> | ||
<h4>Experimental plan to test designs</h4> | <h4>Experimental plan to test designs</h4> | ||
| − | < | + | <ul class="collapsible" data-collapsible="expandable"> |
| − | + | <li><div class="collapsible-header">8.1 How do we detect cellulase activity?</div> | |
| − | </ | + | <div class="collapsible-body"></div></li> |
| + | <li><div class="collapsible-header">8.2 How do we detect styrene production?</div> | ||
| + | <div class="collapsible-body"></div></li> | ||
| + | <li><div class="collapsible-header">8.3 How do we test our strain?</div> | ||
| + | <div class="collapsible-body"></div></li> | ||
| + | </ul> | ||
</div> | </div> | ||
</div> | </div> | ||
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<h4>Upscaling towards a bioreactor and styrene harvesting</h4> | <h4>Upscaling towards a bioreactor and styrene harvesting</h4> | ||
| − | < | + | <ul class="collapsible" data-collapsible="expandable"> |
| − | + | <li><div class="collapsible-header">9.1 How to sustainably upscale towards application in a bioreactor</div> | |
| − | </ | + | <div class="collapsible-body"></div></li> |
| + | |||
| + | </ul> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 11:33, 16 October 2018
In the process of designing the route from cellulose to StyGreen, there are many factors to consider. There are several approaches that can be taken to degrade cellulose, metabolic pathways to styrene as well as different interesting sources of cellulose. Discussions with many experts have aided us in making the right choices or adjustments during the design process. Furthermore, we connected with the upstream and downstream processes of our design and approached different stakeholders, connecting our project to cellulose suppliers and styrene buyers.
The production of StyGreen from cellulose is a multi-step pathway. As such, we have the choice of using either one or multiple organisms to perform the different reactions. For example, we could engineer a cellulose degrading organism and a styrene producing organism; these organisms can be co-cultured to produce styrene from cellulose. However, after discussing this idea with experts, we concluded that creation of a single organism performing both steps would be the most efficient and industrially desirable choice. In this manner, we produce a consolidated bioprocessing system which is not currently available. The combined process saves substrate, raw materials and utilities such as separate cellulase enzyme production. Moreover, the complete process could take place in one reactor and, in case of higher overall efficiency, this results in a reduced reactor volume and thus lower capital investment [A]. Hence, our ultimate goal is the production of a single robust cell factory that can grow on cellulose and produce styrene effectively in an industrial bioreactor setting.
Besides ‘wet lab’ experiments, we also modelled parts of our system in the 'dry lab' with help from experts to substantiate or improve our design. Want to know more details about our design? Click on the questions below to find out!
Design
