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Revision as of 11:38, 16 October 2018


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1.IRF7

Though our computational modeling and network construction using public datasets retrieved from GEO(GSE76967,GSE32186,GSE40644,GSE8807,GSE8923,GSE3568) , we identified IRF7 as our candidate responsible for the high titration feature of cells. We increased virus titer over 2 folds by suppressing IRF7 expression in MDBK cells. In addition, by exposing IRF7-silenced MDBK cells to cold atmospheric plasma, we further increased virus titer to 2.5 fold. Taken, together, through genetic modification and physical plasma treatment, we could increase virus titer up to 5 folds.

Figure 1A. IRF7 gene expression with different SiRNA concentration.

Figure 1B.relative amount of viral DNA with different SiRNA concentration.

C: control NC: negative control
SiRF7-10nmol: SiRNA final concentration 10nmol/ml
SiRF7-50nmol: SiRNA final concnetration 50nmol/ml

Figure 1A showed that IRF7 was effectively knocked down, and Figure 1B showed that Bovine rhinotracheitis virus (IBRV) replication in the experimental group was over 2 folds than that in the negative control group after suppressing IRF7 expression (when siRNA concentration was 50nmol/ml).

2.Plasma

Figure 2 relative amount of viral DNA with different plasma treatment time.

After incubating IRF7 silenced cells with the DMEM medium for 1 hour, IBRV replication in MDBK was further increased 2.5 folds.