Difference between revisions of "Team:NAU-CHINA/testh"

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More future application prospects
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1.What is Mosfet?
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In addition, by replacing the identification object of the upstream system, the measurement of a certain substance can be realized. By adjusting the types of downstream promoters and recombinases, the system threshold can be adjusted.Then the system can not only detect the presence or absence of substances but also dynamically detect the concentration of the detected substances .
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The metal-oxide-semiconductor field-effect transistor (MOSFET, MOS-FET, or MOS FET) is a type of field-effect transistor (FET). It has an insulated gate, whose voltage determines the conductivity of the device. This capacity to change conductivity by varying the amount of applied voltage can be used for amplifying or switching electronic signals. In an enhancement MOSFET mode, voltage applied to the gate terminal increases the conductivity of the device. In depletion mode transistors, voltage applied at the gate reduces the conductivity.
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If we install our system in two kinds of cells so that they can recognize each other and inhibit each other, then we can realize the function of group sensing, which can keep the number of two kinds of cells fluctuating within a certain range. And this will play a important role in industrial production, material detection and other fields. If the cells are made to emit fluorescence, the periodic brightness changes of fluorescence can be seen through periodic fluctuations in the number of cells, thus realizing the function of repressilator.
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The MOSFET is by far the most common transistor in digital circuits,. Millions of transistors may be included in a memory chip or microprocessor.
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2.Why are our cells also called Mosfet?
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Inspired by electronic Mosfet, we designed our project, Monitoring and Operating System Founded on Engineered T cells, which is very similar to Mosfet in many ways. Our MOSFET can be divided into two systems, signal detection system and signal response system.
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Biosafety and biosecurity exam
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We stipulate that only those who have passed the online security exam can enter the laboratory. The safety test questions are determined by the School Experimental Safety Committee and cover the daily safety and biosafety of the laboratory.
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1)Signal detection
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We use synthetic Notch (synNotch) as our signal detection receptor of the engineered T cell. When the recognition domain binds to its target antigen, it will release the effector domain (ED) and the ED is TEV protease.
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2) Signal processing
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We want to convert the extracellular analog signal into an intracellular digital signal . We use Tet operator (TetO) to achieve this goal. This part contains TetO, TEV protease from the ED of synNotch and Tet repressor (TetR) from 2017 Oxford University iGEM project. We can initially implement the filtering function by using these elements but also has leakage because of TetO and TetR. To solve the leakage problem, we introduced a recombinase into the system. The recombinase is capable of flipping the 5' end to the 3' end of the target sequence containing the specific sites, thereby realizing the zero-one switch precisely.
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3) Signal output
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Finally, we achieved equal outputs by using promoters which have different strength and recombinases which have different effects . Because the promoter was located at downstream of TetO and upstream of recombinase, the function is self-evident (if you want to see more, you can click here).
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4) Reset
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When the external signal disappears or falls below the threshold, our mosfet has a reset function, which is to restore the initial state. This function is achieved by a protein called recombination directionality factor (RDF).
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5) The relationship between electronic Mosfet and our MOSFET
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From another perspective, the original Mosfet loaded into the circuit can do the same thing as our Mosfet: 1) constantly detect the voltage signal, 2) to process voltage signal(change the resistance),3) output the corresponding current, and 4) immediately restore the original state after the external changed voltage is restored. But the principles to achieve these are completely different, so the extension must be combined with the actual situation.

Revision as of 11:46, 16 October 2018

1.What is Mosfet? The metal-oxide-semiconductor field-effect transistor (MOSFET, MOS-FET, or MOS FET) is a type of field-effect transistor (FET). It has an insulated gate, whose voltage determines the conductivity of the device. This capacity to change conductivity by varying the amount of applied voltage can be used for amplifying or switching electronic signals. In an enhancement MOSFET mode, voltage applied to the gate terminal increases the conductivity of the device. In depletion mode transistors, voltage applied at the gate reduces the conductivity.

The MOSFET is by far the most common transistor in digital circuits,. Millions of transistors may be included in a memory chip or microprocessor.

2.Why are our cells also called Mosfet? Inspired by electronic Mosfet, we designed our project, Monitoring and Operating System Founded on Engineered T cells, which is very similar to Mosfet in many ways. Our MOSFET can be divided into two systems, signal detection system and signal response system.

Biosafety and biosecurity exam We stipulate that only those who have passed the online security exam can enter the laboratory. The safety test questions are determined by the School Experimental Safety Committee and cover the daily safety and biosafety of the laboratory.

1)Signal detection We use synthetic Notch (synNotch) as our signal detection receptor of the engineered T cell. When the recognition domain binds to its target antigen, it will release the effector domain (ED) and the ED is TEV protease.

2) Signal processing We want to convert the extracellular analog signal into an intracellular digital signal . We use Tet operator (TetO) to achieve this goal. This part contains TetO, TEV protease from the ED of synNotch and Tet repressor (TetR) from 2017 Oxford University iGEM project. We can initially implement the filtering function by using these elements but also has leakage because of TetO and TetR. To solve the leakage problem, we introduced a recombinase into the system. The recombinase is capable of flipping the 5' end to the 3' end of the target sequence containing the specific sites, thereby realizing the zero-one switch precisely.

3) Signal output Finally, we achieved equal outputs by using promoters which have different strength and recombinases which have different effects . Because the promoter was located at downstream of TetO and upstream of recombinase, the function is self-evident (if you want to see more, you can click here).

4) Reset When the external signal disappears or falls below the threshold, our mosfet has a reset function, which is to restore the initial state. This function is achieved by a protein called recombination directionality factor (RDF).

5) The relationship between electronic Mosfet and our MOSFET From another perspective, the original Mosfet loaded into the circuit can do the same thing as our Mosfet: 1) constantly detect the voltage signal, 2) to process voltage signal(change the resistance),3) output the corresponding current, and 4) immediately restore the original state after the external changed voltage is restored. But the principles to achieve these are completely different, so the extension must be combined with the actual situation.