Difference between revisions of "Team:Virginia"

Line 1: Line 1:
−
<!DOCTYPE html>
+
 
 
<html>
 
<html>
−
<head>
+
 
 
   <meta charset="utf-8">
 
   <meta charset="utf-8">
 
   <meta name="viewport" content="width=device-width, initial-scale=1">
 
   <meta name="viewport" content="width=device-width, initial-scale=1">
 
   <!--link rel="stylesheet" href="dist/css/vendor.css"-->
 
   <!--link rel="stylesheet" href="dist/css/vendor.css"-->
−
   <script src="dist/js/vendor.js"></script>
+
   <script src="https://2018.igem.org/Template:Virginia/js/vendor?action=raw&ctype=text/javascript"></script>
−
   <link rel="stylesheet" href="css/styles.css">
+
   <link rel="stylesheet" href="https://2018.igem.org/Template:Virginia/css/styles?action=raw&ctype=text/css">
−
   <link rel="stylesheet" href="css/index.css">
+
   <link rel="stylesheet" href="https://2018.igem.org/Template:Virginia/css/index?action=raw&ctype=text/css">
−
   <script src="js/wiki.js"></script>
+
   <script src="https://2018.igem.org/Template:Virginia/js/wiki?action=raw&ctype=text/javascript"></script>
−
   <!--script>
+
   <script>
 
     $(function() {
 
     $(function() {
−
       $('#header').load('./templates/header.html');
+
       $('#header').load('https://2018.igem.org/Template:Virginia/header?action=raw&ctype=text/javascript');
−
       $('#footer').load('./templates/footer.html');
+
       $('#footer').load('https://2018.igem.org/Template:Virginia/footer?action=raw&ctype=text/javascript');
 
     });
 
     });
−
   </script-->
+
   </script>
−
</head>
+
 
  
 
<!-- NAVIGATION BAR -->
 
<!-- NAVIGATION BAR -->
  
−
<body>
+
 
−
    <header>
+
 
−
        <nav role="navigation">
+
  <div id="header"></div>
−
            <ul>
+
   <div id="main" class="thetop">
−
                <li>
+
      <!--Images-->
−
                    <a class="internal" href="index.html">
+
−
                        <img src="images/Wireframe_Logo_Light.png" alt="UVA logo"></img>  Hivemind - Home
+
−
                    </a>
+
−
                </li>
+
−
                <li>
+
−
                    <a href="#">Project</a>
+
−
                    <ul>
+
−
                        <li><a class="internal" href="Overview_Project.html">Overview</a></li>
+
−
                        <li><a class="internal" href="Description.html">Description</a></li>
+
−
                        <li><a class="internal" href="Design.html">Design</a></li>
+
−
                        <li><a class="internal" href="Experiments.html">Experiments</a></li>
+
−
                        <li><a class="internal" href="Notebook.html">Notebook</a></li>
+
−
                        <li><a class="internal" href="InterLab.html">InterLab</a></li>
+
−
                        <li><a class="internal" href="Model.html">Model</a></li>
+
−
                        <li><a class="internal" href="Results.html">Results</a></li>
+
−
                        <li><a class="internal" href="Demonstrate.html">Demonstrate</a></li>
+
−
                        <li><a class="internal" href="Improve.html">Improve</a></li>
+
−
                    </ul>
+
−
                </li>
+
−
                <li>
+
−
                    <a href="#">Parts</a>
+
−
                    <ul>
+
−
                        <li><a class="internal" href="Parts.html">Overview</a></li>
+
−
                        <li><a class="internal" href="Basic_Part.html">Basic Parts</a></li>
+
−
                        <li><a class="internal" href="Composite_Part.html">Composite Parts</a></li>
+
−
                        <li><a class="internal" href="Part_Collection.html">Part Collection</a></li>
+
−
                    </ul>
+
−
                </li>
+
−
                <li>
+
−
                    <a class="internal" href="Safety.html">Safety</a>
+
−
                </li>
+
−
                <li>
+
−
                    <a href="#">Human Practices</a>
+
−
                    <ul>
+
−
                        <li><a class="internal" href="Overview_HP.html">Overview</a></li>
+
−
                        <li><a class="internal" href="Human_Practices.html">Human Practices</a></li>
+
−
                        <li><a class="internal" href="Public_Engagement.html">Public Engagement</a></li>
+
−
                    </ul>
+
−
                </li>
+
−
                <li>
+
−
                    <a href="#">Team</a>
+
−
                    <ul>
+
−
                        <li><a class="internal" href="Team.html">Team Members</a></li>
+
−
                        <li><a class="internal" href="Collaborations.html">Collaborations</a></li>
+
−
                        <li><a class="internal" href="Attributions.html">Attributions</a></li>
+
−
                    </ul>
+
−
                </li>
+
−
                <li>
+
−
                    <a href="#">Awards</a>
+
−
                    <ul>
+
−
                        <li><a class="internal" href="Applied_Design.html">Applied Design</a></li>
+
−
                        <li><a class="internal" href="Entrepreneurship.html">Entrepreneurship</a></li>
+
−
                        <li><a class="internal" href="Hardware.html">Hardware</a></li>
+
−
                        <li><a class="internal" href="Measurement.html">Measurement</a></li>
+
−
                        <li><a class="internal" href="Model.html">Model</a></li>
+
−
                        <!--li><a class="internal" href="Plant.html">Plant</a></li-->
+
−
                        <li><a class="internal" href="Software.html">Software</a></li>
+
−
                    </ul>
+
−
                </li>
+
−
                <li>
+
−
                    <a href="https://igem.org/2018_Judging_Form?team=Virginia.html">Judging Form ⇗</a>
+
−
                </li>
+
−
            </ul>
+
−
        </nav>
+
−
    </header>
+
−
   <div id="content" class="thetop">
+
−
        <!--Images-->
+
 
     <div id="text">
 
     <div id="text">
 
       <h1> Project Description</h1>
 
       <h1> Project Description</h1>
Line 105: Line 38:
 
   <div class="category-gallery">
 
   <div class="category-gallery">
 
     <div>
 
     <div>
−
       <a class="internal" href="Team.html">
+
       <a class="internal" href="https://2018.igem.org/Team:Virginia/Team">
−
         <img src="images/vgem-logo.png" alt="Team">
+
         <img src="https://static.igem.org/mediawiki/2018/1/13/T--Virginia--2018_vgem-logo.png" alt="Team">
 
         <p><strong>Team</strong></p>
 
         <p><strong>Team</strong></p>
 
       </a>
 
       </a>
 
     </div>
 
     </div>
 
     <div>
 
     <div>
−
       <a class="internal" href="Description.html">
+
       <a class="internal" href="https://2018.igem.org/Team:Virginia/Description">
−
         <img src="images/vgem-logo.png" alt="Project">
+
         <img src="https://static.igem.org/mediawiki/2018/1/13/T--Virginia--2018_vgem-logo.png" alt="Project">
 
         <p><strong>Project</strong></p>   
 
         <p><strong>Project</strong></p>   
 
       </a>
 
       </a>
 
     </div>
 
     </div>
 
     <div>
 
     <div>
−
       <a class="internal" href="Parts.html">
+
       <a class="internal" href="https://2018.igem.org/Team:Virginia/Parts">
−
         <img src="images/vgem-logo.png" alt="Parts">
+
         <img src="https://static.igem.org/mediawiki/2018/1/13/T--Virginia--2018_vgem-logo.png" alt="Parts">
 
         <p><strong>Parts</strong></p>
 
         <p><strong>Parts</strong></p>
 
       </a>
 
       </a>
 
     </div>
 
     </div>
 
     <div>
 
     <div>
−
       <a class="internal" href="Safety.html">
+
       <a class="internal" href="https://2018.igem.org/Team:Virginia/Safety">
−
         <img src="images/vgem-logo.png" alt="Safety">
+
         <img src="https://static.igem.org/mediawiki/2018/1/13/T--Virginia--2018_vgem-logo.png" alt="Safety">
 
         <p><strong>Safety</strong></p>
 
         <p><strong>Safety</strong></p>
 
       </a>
 
       </a>
 
     </div>
 
     </div>
 
     <div>
 
     <div>
−
       <a class="internal" href="Human_Practices.html">
+
       <a class="internal" href="https://2018.igem.org/Team:Virginia/Human_Practices">
−
         <img src="images/vgem-logo.png" alt="Human Practices">
+
         <img src="https://static.igem.org/mediawiki/2018/1/13/T--Virginia--2018_vgem-logo.png" alt="Human Practices">
 
         <p><strong>Human Practices</strong></p>
 
         <p><strong>Human Practices</strong></p>
 
       </a>
 
       </a>
 
     </div>
 
     </div>
 
     <div>
 
     <div>
−
       <a class="internal" href="Awards.html">
+
       <a class="internal" href="https://2018.igem.org/Team:Virginia/Awards">
−
         <img src="images/vgem-logo.png" alt="Awards">
+
         <img src="https://static.igem.org/mediawiki/2018/1/13/T--Virginia--2018_vgem-logo.png" alt="Awards">
 
         <p><strong>Awards</strong></p>
 
         <p><strong>Awards</strong></p>
 
       </a>
 
       </a>
Line 142: Line 75:
 
   </div>
 
   </div>
  
−
   <footer>
+
   <div id="footer"></div>
−
    <div class="footer-name">
+
−
      <img class="logo" src="images/Wireframe_Logo_Light.png" alt="Virginia iGEM Logo">
+
−
      <h3>Virginia iGEM 2018</h3>
+
−
    </div>
+
−
    <div class="spacer">
+
−
      <!--
+
−
      <a href="#top">
+
−
        <h3>▲ To top</h3>
+
−
      </a>
+
−
      -->
+
−
    </div>
+
−
<!--Code courtesy of https://codepen.io/brandondward/pen/RpyaKL-->
+
−
    <div class="social-container">
+
−
      <ul class="social-icons">
+
−
          <li><a href="https://twitter.com/virginia_igem?lang=en"><i class="fa fa-twitter"></i></a></li>
+
−
          <li><a href="https://github.com/Mantissa-23/VGEM-2018"><i class="fa fa-github"></i></a></li>
+
−
          <li><a href="https://www.facebook.com/virginiaigem/"><i class="fa fa-facebook"></i></a></li>
+
−
          <li><a href="mailto:virginia.igem@gmail.com"><i class="fa fa-envelope-o"></i></a></li>
+
−
      </ul>
+
−
    </div>
+
−
  </footer>
+
−
 
+
 
   <!--SMOOTH SCROLL UP-->
 
   <!--SMOOTH SCROLL UP-->
−
   <div class='scrolltop'>
+
   <div class="scrolltop">
−
     <div class='scroll icon'><i class="fa fa-4x fa-angle-up"></i></div>
+
     <div class="scroll icon"><i class="fa fa-4x fa-angle-up"></i></div>
 
   </div>
 
   </div>
−
</body>
+
 
 
</html>
 
</html>

Revision as of 16:10, 26 June 2018

Project Description

Heterogeneity of cell populations caused by quorum sensing leads to variability in gene expression that is hard to predict. During biomanufacturing, elevating protein expression will lead to gain of profit. Decreasing protein expression can also be beneficial in situations where undesirable biofilms may form on medical equipment or controlling virulence in bacteria. We will modify the existing bacteria Autoinducer-2 (AI-2) quorum sensing system by upregulating AI-2 synthesis and excretion coupled with AI-2 uptake and phosphorylation after the initial AI-2 threshold concentrations have been reached to reduce variability in induced gene expression. A model is used to predict the impacts of manipulating the expression of quorum sensing genes and guide design and experimentation. DNA assembly of a Biobrick containing pLsr, T7RPol, LsrK, LsrACDB, LuxS, YdgG, and superfolding Green Fluorescent Protein (sfGFP) enables the quantification of what percent of cells are expressing the synthetic plasmid and their levels of protein production. This device will improve the viability of autoinduction as an induction method in industries such as biomanufacturing by decreasing the variability of a cell phenotypes within a single culture to reduce costs leading to an increase in profits.