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Revision as of 09:41, 27 June 2018
Description
1.Nicotine (1-methyl-2-[3-pyridyl-pyrrolidine], C10H14N2), playing a major role in tobaccos, is an indispensable factor in producing cigarettes. Smoking addiction and is well known addiction due to the intake nicotine contained in cigarettes. Intake of nicotine could cause serious problems in human body, because it easily crosses the blood-brain barrier and biological membranes. In another case, as customers demand for cigarettes, massive amount of tobacco were being processed, this caused tobacco waste. Aminobiphenyl, naphthylamine, and benzo(a)- pyrene, were all examples of pollution that created and entered in the environment. Moreover, nicotine and many alkaloids are all not recyclable, so when those are being discharged, they brings a big impact on environment and causes pollution.
2.Recently, there were a lot more nicotine-degrading bacteria researched being reported, and the degradation pathways of nicotine was more and more clear. The degradation of nicotine through the pyrrolidine pathway by Pseudomonas is extensive and well studied.
3.In our study, the first three gene_____,_______and______(增加三个基因的名字和基因序列号) are crucial for nicotine degradation, so we have construct a pathway to express the three gene in top10.
Nicotine degradation
Gene cloning
Pathway construction
Vector construction
Protein expression
Screening
Nicotine catalysis
以上除了图片是所有项目介绍内容
What should this page contain?
- A clear and concise description of your project.
- A detailed explanation of why your team chose to work on this particular project.
- References and sources to document your research.
- Use illustrations and other visual resources to explain your project.
Advice on writing your Project Description
We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements.
References
iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.