Difference between revisions of "Team:BCU/Parts"
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| − | <span | + | <span><a href="https://2018.igem.org/Team:BCU/Description">PROJECT</a></span> |
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<div class="dropdown dropdownPlus"> | <div class="dropdown dropdownPlus"> | ||
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</div> | </div> | ||
<div class="main"> | <div class="main"> | ||
| + | <div class="pContent"> | ||
<h1>Parts</h1> | <h1>Parts</h1> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/1/11/T--BCU--suibian06.gif"> | ||
<h2>Basic Parts</h2> | <h2>Basic Parts</h2> | ||
| − | + | <p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2567000">BBa_K2567000</a>: nicA (Nicotine oxidoreductase)</p> | |
| − | + | ||
| − | The part code for the nicA protein. This protein can degrade nicotine. Nicotine oxidoreductase (nicA) from Pseudomonas putida S16 is the first enzyme in the pyrrolidine pathway for nicotine degradation. | + | |
| − | We optimization the nicA in order to express protein successfully. Including but not limited to: | + | <p>The part code for the nicA protein. This protein can degrade nicotine. Nicotine oxidoreductase (nicA) from Pseudomonas putida S16 is the first enzyme in the pyrrolidine pathway for nicotine degradation.</p> |
| − | • Codon usage bias | + | <p>We optimization the nicA in order to express protein successfully. Including but not limited to:</p> |
| − | • GC content | + | <p>• Codon usage bias</p> |
| − | • CpG dinucleotides content | + | <p>• GC content</p> |
| − | • mRNA secondary structure | + | <p>• CpG dinucleotides content</p> |
| − | • Cryptic splicing sites | + | <p>• mRNA secondary structure</p> |
| − | • Premature PolyA sites | + | <p>• Cryptic splicing sites</p> |
| − | • Internal chi sites and ribosomal binding sites | + | <p>• Premature PolyA sites</p> |
| − | • Negative CpG islands | + | <p>• Internal chi sites and ribosomal binding sites</p> |
| − | • RNA instability motif (ARE) | + | <p>• Negative CpG islands</p> |
| − | • Repeat sequences (direct repeat, reverse repeat, and Dyad repeat) | + | <p>• RNA instability motif (ARE)</p> |
| − | • Restriction sites that may interfere with cloning | + | <p>• Repeat sequences (direct repeat, reverse repeat, and Dyad repeat)</p> |
| − | Additional sequences we propose to improve translational performance | + | <p>• Restriction sites that may interfere with cloning</p> |
| − | (1) To increase the efficiency of translational initiation | + | <p>Additional sequences we propose to improve translational performance</p> |
| − | • Kozak sequence | + | <p>(1) To increase the efficiency of translational initiation</p> |
| − | • Shine-Dalgarno Sequence | + | <p>• Kozak sequence</p> |
| − | (2) To increase the efficiency of translational termination | + | <p>• Shine-Dalgarno Sequence</p> |
| − | • Stop codon(TAA) | + | <p>(2) To increase the efficiency of translational termination</p> |
| − | + | <p>• Stop codon(TAA)</p> | |
| − | Composite Parts | + | </div> |
| − | + | <img src="https://static.igem.org/mediawiki/2018/8/89/T--BCU--parts.png"> | |
| − | + | <br/> | |
| − | Use a promoter (BBa_J23119), ribosome binding site (BBa_B0034), nicA coding region (BBa_K2567000), terminator(BBa_B0015). This part can express nicA, and nicA can degrade nicotine. | + | <div class="pContent"> |
| + | |||
| + | <h2>Composite Parts</h2> | ||
| + | |||
| + | <p><a href="http://parts.igem.org/Part:BBa_K2567001">BBa_K2567001</a>: express nicA and degrade nicotine</p> | ||
| + | |||
| + | <p>Use a promoter (BBa_J23119), ribosome binding site (BBa_B0034), nicA coding region (BBa_K2567000), terminator(BBa_B0015). This part can express nicA, and nicA can degrade nicotine. | ||
| + | </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/4/40/T--BCU--xiabianlan02.jpg"> | ||
| + | </div> | ||
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> | ||
Latest revision as of 16:25, 16 October 2018
EXPERIMENT
Parts
Basic Parts
BBa_K2567000: nicA (Nicotine oxidoreductase)
The part code for the nicA protein. This protein can degrade nicotine. Nicotine oxidoreductase (nicA) from Pseudomonas putida S16 is the first enzyme in the pyrrolidine pathway for nicotine degradation.
We optimization the nicA in order to express protein successfully. Including but not limited to:
• Codon usage bias
• GC content
• CpG dinucleotides content
• mRNA secondary structure
• Cryptic splicing sites
• Premature PolyA sites
• Internal chi sites and ribosomal binding sites
• Negative CpG islands
• RNA instability motif (ARE)
• Repeat sequences (direct repeat, reverse repeat, and Dyad repeat)
• Restriction sites that may interfere with cloning
Additional sequences we propose to improve translational performance
(1) To increase the efficiency of translational initiation
• Kozak sequence
• Shine-Dalgarno Sequence
(2) To increase the efficiency of translational termination
• Stop codon(TAA)
Composite Parts
BBa_K2567001: express nicA and degrade nicotine
Use a promoter (BBa_J23119), ribosome binding site (BBa_B0034), nicA coding region (BBa_K2567000), terminator(BBa_B0015). This part can express nicA, and nicA can degrade nicotine.
