(Prototype team page) |
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+ | <html xmlns="http://www.w3.org/1999/xhtml"> | ||
+ | <head> | ||
+ | <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> | ||
+ | <title>无标题文档</title> | ||
+ | <style type="text/css"> | ||
+ | <!-- | ||
+ | body { | ||
+ | margin-left: 0px; | ||
+ | margin-top: 0px; | ||
+ | margin-right: 0px; | ||
+ | margin-bottom: 0px; | ||
+ | background-color: white !important; | ||
+ | min-width: 960px !important; | ||
+ | overflow-x: hidden !important; | ||
+ | } | ||
+ | .title { | ||
+ | height: 240px; | ||
+ | width: 960px; | ||
+ | margin-right: auto; | ||
+ | margin-left: auto; | ||
+ | |||
+ | border-right-style: none; | ||
+ | |||
+ | border-left-style: none; | ||
+ | |||
+ | } | ||
+ | .nav { | ||
+ | float: right; | ||
+ | height: 80px; | ||
+ | width: 960px; | ||
+ | margin-top: 35px; | ||
+ | font-size: 16px; | ||
+ | color: #1C2C77; | ||
+ | border-top-width: thin; | ||
+ | font-weight: 800; | ||
+ | font-family: Arial, Helvetica, sans-serif; | ||
+ | font-style: normal; | ||
+ | line-height: 40px; | ||
+ | text-align: center; | ||
+ | } | ||
+ | .main { | ||
+ | height: 400px; | ||
+ | width: 960px; | ||
+ | margin-right: auto; | ||
+ | margin-left: auto; | ||
+ | border-top-width: 5px; | ||
+ | border-top-style: none; | ||
+ | border-top-color: #FC0; | ||
+ | background-image: url(); | ||
+ | background-attachment: fixed; | ||
+ | background-repeat: no-repeat; | ||
+ | background-position: center center; | ||
+ | font-family: "Palatino Linotype", "Book Antiqua", Palatino, serif; | ||
+ | color: #FFF; | ||
+ | text-decoration: blink; | ||
+ | font-weight: 900; | ||
+ | text-transform: capitalize; | ||
+ | text-align: left !important; | ||
+ | padding-top: 0px; | ||
+ | font-size: larger; | ||
+ | margin-bottom: 3px; | ||
+ | } | ||
+ | .main .pContent p{ | ||
+ | color: black !important; | ||
+ | text-indent:2em !important; | ||
+ | font-size:16px !important; | ||
+ | font-weight:normal !important; | ||
+ | line-height:35px !important; | ||
+ | } | ||
+ | .main .pContent h1,h2{ | ||
+ | text-align: left !important; | ||
+ | color: black !important; | ||
+ | } | ||
+ | .pContent{ | ||
+ | margin-left: 30px !important; | ||
+ | width: 900px !important; | ||
+ | height: auto; | ||
+ | } | ||
+ | .logo { | ||
+ | float: left; | ||
+ | height: 130px; | ||
+ | width: 300px; | ||
+ | background-repeat: no-repeat; | ||
+ | margin: 10px; | ||
+ | } | ||
+ | html { | ||
+ | font-family: sans-serif; | ||
+ | -webkit-text-size-adjust: 100%; | ||
+ | -ms-text-size-adjust: 100%; | ||
+ | } | ||
+ | body { | ||
+ | margin: 0; | ||
+ | font-family: "Microsoft Yahei", Helvetica, Arial, sans-serif; | ||
+ | } | ||
+ | a { | ||
+ | text-decoration: none; | ||
+ | } | ||
+ | a:hover, | ||
+ | a:focus { | ||
+ | text-decoration: none; | ||
+ | } | ||
+ | a:focus { | ||
+ | outline: thin dotted; | ||
+ | outline: 5px auto -webkit-focus-ring-color; | ||
+ | outline-offset: -2px; | ||
+ | } | ||
+ | |||
+ | .container{ | ||
+ | width: 1200px; | ||
+ | min-width: 1200px; | ||
+ | margin-right: auto; | ||
+ | margin-left: auto; | ||
+ | } | ||
+ | |||
+ | .shortcut{ | ||
+ | height: 24px; | ||
+ | line-height: 24px; | ||
+ | padding-top: 16px; | ||
+ | } | ||
+ | .shortcut a{ | ||
+ | color: #808080; | ||
+ | -webkit-transition: all 0.3s ease 0s; | ||
+ | -moz-transition: all 0.3s ease 0s; | ||
+ | -o-transition: all 0.3s ease 0s; | ||
+ | transition: all 0.3s ease 0s; | ||
+ | } | ||
+ | .shortcut a img{ | ||
+ | margin-right: 5px; | ||
+ | } | ||
+ | .shortcut a:hover{ | ||
+ | color: #8e2322; | ||
+ | } | ||
+ | .header{ | ||
+ | width: 100%; | ||
+ | min-width: 1200px; | ||
+ | height: 110px; | ||
+ | background: #fff; | ||
+ | } | ||
+ | .logo img{ | ||
+ | display: block; | ||
+ | width: 500px; | ||
+ | margin-right: auto; | ||
+ | margin-left: auto; | ||
+ | } | ||
+ | |||
+ | /*nav*/ | ||
+ | nav a{ | ||
+ | display: inline-block; | ||
+ | height: 38px; | ||
+ | line-height: 38px; | ||
+ | margin-right: 43px; | ||
+ | margin-left: 43px; | ||
+ | font-size: 16px; | ||
+ | color: #333; | ||
+ | -webkit-transition: all 0.3s ease 0s; | ||
+ | -moz-transition: all 0.3s ease 0s; | ||
+ | -o-transition: all 0.3s ease 0s; | ||
+ | transition: all 0.3s ease 0s; | ||
+ | } | ||
+ | nav a:hover, | ||
+ | nav .on{ | ||
+ | color: #8e2322; | ||
+ | } | ||
+ | #content #top_title{ | ||
+ | display:none; | ||
+ | } | ||
+ | #content{ | ||
+ | width: 100%; | ||
+ | padding: 0; | ||
+ | } | ||
+ | .mod-01 img{ | ||
+ | display: block; | ||
+ | margin-right: auto; | ||
+ | margin-left: auto; | ||
+ | } | ||
+ | <!--Menu Part--> | ||
+ | .dropdown { | ||
+ | position: relative; | ||
+ | display: inline-block; | ||
+ | background-color: white; | ||
+ | color: #040059; | ||
+ | float: left; | ||
+ | } | ||
+ | .dropdownPlus{ | ||
+ | float: left; | ||
+ | height: 80px !important; | ||
+ | min-width:50px !important; | ||
+ | display: inline-block !important; | ||
+ | margin-left:6px !important; | ||
+ | margin-right:13.5px !important; | ||
+ | padding-top: 35px !important; | ||
+ | font-family: "Microsoft Yahei", Helvetica, Arial, sans-serif; | ||
+ | } | ||
+ | .dropdownPlus span a{ | ||
+ | color: #1C2C77 !important; | ||
+ | } | ||
+ | .dropdownPlus span a:hover{ | ||
+ | color: white !important; | ||
+ | } | ||
+ | .dropdown span{ | ||
+ | width: 120px !important; | ||
+ | } | ||
+ | .dropdown-content { | ||
+ | display: none; | ||
+ | position: absolute; | ||
+ | background-color: #f9f9f9; | ||
+ | min-width: 195px; | ||
+ | box-shadow: 0px 8px 16px 0px rgba(0,0,0,0.2); | ||
+ | padding: 12px 0px; | ||
+ | text-align: left !important; | ||
+ | } | ||
+ | .dropdown span:hover{ | ||
+ | background-color: #040059; | ||
+ | color: white; | ||
+ | } | ||
+ | .dropdown:hover .dropdown-content { | ||
+ | width: 160px !important; | ||
+ | display: block; | ||
+ | } | ||
+ | .dropdown-content p{ | ||
+ | padding-left: 16px; | ||
+ | line-height: 35px !important; | ||
+ | margin: 0px !important; | ||
+ | } | ||
+ | .dropdown-content p a{ | ||
+ | color: #040059 !important; | ||
+ | } | ||
+ | .dropdown-content p:nth-child(2n){ | ||
+ | background:#EEEEEE !important; | ||
+ | } | ||
+ | .dropdown-content p:hover{ | ||
+ | background-color: #85cafa !important; | ||
+ | } | ||
+ | .dropdown-content p:hover a{ | ||
+ | color: white !important; | ||
+ | } | ||
+ | .logo-title{ | ||
+ | text-align: center; | ||
+ | } | ||
+ | .logo-title img{ | ||
+ | |||
+ | } | ||
+ | .main img{ | ||
+ | width: 960px !important; | ||
+ | } | ||
+ | #globalWrapper{ | ||
+ | margin: 0; | ||
+ | padding: 0; | ||
+ | } | ||
+ | #content{ | ||
+ | width: 100%; | ||
+ | padding: 0; | ||
+ | margin: -12px 0 0; | ||
+ | } | ||
+ | #top_title{ | ||
+ | display: none; | ||
+ | } | ||
+ | #sideMenu{ | ||
+ | display: none; | ||
+ | } | ||
+ | #content, | ||
+ | .vs-content, | ||
+ | .vs-wrapper section { | ||
+ | background-color: transparent !important; | ||
+ | } | ||
+ | .my-backTop:visited { | ||
+ | important; | ||
+ | } | ||
+ | /*以上CSS用于解决iGEM官网坑爹样式问题*/ | ||
+ | </style> | ||
+ | </head> | ||
+ | <body> | ||
+ | <div class="title"> | ||
+ | <div class="nav"> | ||
+ | <div class="dropdown dropdownPlus"> | ||
+ | <span><a href="https://2018.igem.org/Team:BCU">HOME</a></span> | ||
+ | </div> | ||
+ | <div class="dropdown dropdownPlus"> | ||
+ | <span><a href="https://2018.igem.org/Team:BCU/Team">TEAM</a></span> | ||
+ | </div> | ||
+ | <div class="dropdown dropdownPlus"> | ||
+ | <span><a href="https://2018.igem.org/Team:BCU/Description">PROJECT</a></span> | ||
+ | </div> | ||
+ | <div class="dropdown dropdownPlus"> | ||
+ | <span>EXPERIMENT</span> | ||
+ | <div class="dropdown-content"> | ||
+ | <p><a href="https://2018.igem.org/Team:BCU/Design">Design</a></p> | ||
+ | <p><a href="https://2018.igem.org/Team:BCU/Improve">Materials&Reagents</a></p> | ||
+ | <p><a href="https://2018.igem.org/Team:BCU/Demonstrate">Methods</a></p> | ||
+ | <p><a href="https://2018.igem.org/Team:BCU/Results">Results</a></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="dropdown dropdownPlus" > | ||
+ | <span><a href="https://2018.igem.org/Team:BCU/Parts">PARTS</a></span> | ||
+ | </div> | ||
+ | <img class="dropdownPlus" style="padding-top: 0px !important;width: 83px !important;" src="https://static.igem.org/mediawiki/2018/c/cd/T--BCU--LOGOHEAD.png" /> | ||
+ | <div class="dropdown dropdownPlus" > | ||
+ | <span><a href="https://2018.igem.org/Team:BCU/InterLab">INTERLAB</a></span> | ||
+ | </div> | ||
+ | <div class="dropdown dropdownPlus"> | ||
+ | <span><a href="https://2018.igem.org/Team:BCU/Notebook">NOTEBOOK</a></span> | ||
+ | </div> | ||
+ | <div class="dropdown dropdownPlus"> | ||
+ | <span><a href="https://2018.igem.org/Team:BCU/Attributions">ATTRIBUTION</a></span> | ||
+ | </div> | ||
+ | <div class="dropdown dropdownPlus"> | ||
+ | <span><a href="https://2018.igem.org/Team:BCU/Model">MEDAL</a></span> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <div class="logo-title"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/thumb/7/70/T--BCU--LOGOTITLE.png/320px-T--BCU--LOGOTITLE.png"/> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="main"> | ||
+ | <div class="pContent"> | ||
+ | <h1>Parts</h1> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/1/11/T--BCU--suibian06.gif"> | ||
+ | <h2>Basic Parts</h2> | ||
+ | <p><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2567000">BBa_K2567000</a>: nicA (Nicotine oxidoreductase)</p> | ||
+ | |||
− | < | + | <p>The part code for the nicA protein. This protein can degrade nicotine. Nicotine oxidoreductase (nicA) from Pseudomonas putida S16 is the first enzyme in the pyrrolidine pathway for nicotine degradation.</p> |
− | < | + | <p>We optimization the nicA in order to express protein successfully. Including but not limited to:</p> |
− | <p> | + | <p>• Codon usage bias</p> |
− | <p> | + | <p>• GC content</p> |
+ | <p>• CpG dinucleotides content</p> | ||
+ | <p>• mRNA secondary structure</p> | ||
+ | <p>• Cryptic splicing sites</p> | ||
+ | <p>• Premature PolyA sites</p> | ||
+ | <p>• Internal chi sites and ribosomal binding sites</p> | ||
+ | <p>• Negative CpG islands</p> | ||
+ | <p>• RNA instability motif (ARE)</p> | ||
+ | <p>• Repeat sequences (direct repeat, reverse repeat, and Dyad repeat)</p> | ||
+ | <p>• Restriction sites that may interfere with cloning</p> | ||
+ | <p>Additional sequences we propose to improve translational performance</p> | ||
+ | <p>(1) To increase the efficiency of translational initiation</p> | ||
+ | <p>• Kozak sequence</p> | ||
+ | <p>• Shine-Dalgarno Sequence</p> | ||
+ | <p>(2) To increase the efficiency of translational termination</p> | ||
+ | <p>• Stop codon(TAA)</p> | ||
</div> | </div> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/8/89/T--BCU--parts.png"> | ||
+ | <br/> | ||
+ | <div class="pContent"> | ||
− | < | + | <h2>Composite Parts</h2> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | </ | + | |
− | < | + | <p><a href="http://parts.igem.org/Part:BBa_K2567001">BBa_K2567001</a>: express nicA and degrade nicotine</p> |
− | + | ||
− | + | ||
+ | <p>Use a promoter (BBa_J23119), ribosome binding site (BBa_B0034), nicA coding region (BBa_K2567000), terminator(BBa_B0015). This part can express nicA, and nicA can degrade nicotine. | ||
+ | </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
− | + | <img src="https://static.igem.org/mediawiki/2018/4/40/T--BCU--xiabianlan02.jpg"> | |
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</html> | </html> |
Latest revision as of 16:25, 16 October 2018
Parts
Basic Parts
BBa_K2567000: nicA (Nicotine oxidoreductase)
The part code for the nicA protein. This protein can degrade nicotine. Nicotine oxidoreductase (nicA) from Pseudomonas putida S16 is the first enzyme in the pyrrolidine pathway for nicotine degradation.
We optimization the nicA in order to express protein successfully. Including but not limited to:
• Codon usage bias
• GC content
• CpG dinucleotides content
• mRNA secondary structure
• Cryptic splicing sites
• Premature PolyA sites
• Internal chi sites and ribosomal binding sites
• Negative CpG islands
• RNA instability motif (ARE)
• Repeat sequences (direct repeat, reverse repeat, and Dyad repeat)
• Restriction sites that may interfere with cloning
Additional sequences we propose to improve translational performance
(1) To increase the efficiency of translational initiation
• Kozak sequence
• Shine-Dalgarno Sequence
(2) To increase the efficiency of translational termination
• Stop codon(TAA)
Composite Parts
BBa_K2567001: express nicA and degrade nicotine
Use a promoter (BBa_J23119), ribosome binding site (BBa_B0034), nicA coding region (BBa_K2567000), terminator(BBa_B0015). This part can express nicA, and nicA can degrade nicotine.