Difference between revisions of "Team:OUC-China/Experiments"
| Line 163: | Line 163: | ||
<br /><br /> | <br /><br /> | ||
<div class="chapai"><br /> | <div class="chapai"><br /> | ||
| − | <h4 ><font size="6">Elementary Operation</font></h4 > | + | <h4 ><font size="6">1.Elementary Operation</font></h4 > |
| − | <br /> | + | <br />（1）Conservation |
| − | <br /> | + | <br />（2）Gel Electrophoresis |
| − | <br /> | + | <br />（3）Gel Extraction |
| − | <br /> | + | <br />（4）Inoculation |
| − | <br /> | + | <br />（5）Ligation system |
| − | <br /> | + | <br />（6）medium |
| − | <br /> | + | <br />（7）Plasmid extraction |
| − | <br /> | + | <br />（8）Polymerase Chain Reaction |
| − | <br /> | + | <br />（9）Restriction Enzyme Digestion |
| − | <br /> | + | <br />（10）Sterilization |
| − | <br /> | + | <br />（11）Transformation of Escherichia coli <br /><br /> |
| − | <h4 ><font size="6">Systems Detection</font></h4 > | + | <h4 ><font size="6">2.Systems Detection</font></h4 > |
| − | <br /> | + | <br />（1）Assay of Gene Expression by Flow Cytometry |
| − | <br /> | + | <br />（2）Fluorescence measurement |
| − | <br /> | + | <br />（3）miniToe Polycistron Fluorescence measurement |
| − | <br /> | + | <br />（4）The application of miniToe——Regulation of motA |
<br /><br /> | <br /><br /> | ||
Revision as of 16:26, 16 October 2018
Experiments
This year, our project can be divided into four systems. Between the different systems, there are different protocols. And we have sorted out these experimentals in detail.
1.Elementary Operation
（1）Conservation
（2）Gel Electrophoresis
（3）Gel Extraction
（4）Inoculation
（5）Ligation system
（6）medium
（7）Plasmid extraction
（8）Polymerase Chain Reaction
（9）Restriction Enzyme Digestion
（10）Sterilization
（11）Transformation of Escherichia coli
2.Systems Detection
（1）Assay of Gene Expression by Flow Cytometry
（2）Fluorescence measurement
（3）miniToe Polycistron Fluorescence measurement
（4）The application of miniToe——Regulation of motA
Contact Us : oucigem@163.com | ©2018 OUC IGEM.All Rights Reserved. | …………