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Revision as of 19:14, 16 October 2018
In the worm buster project we have divided our work into separate subgroups to handle the massive burden of the project. To read more about how we designed our experiments and the results we got click on the buttons below. They will take you to each page for the different parts of the wet-lab project.
Our results were succesful, we managed to design a DNA sequence for our alternative reporter, we found interesting sequences using phage display that could be used to create a biosensor and we developed mutiple new applications for existing techniques. In turn this has made it more realistic to create future worm buster tools.
Transcriptomics
we managed to perform our entire pipeline and sequence the E.coli transcriptome five times. However, the sequencing data was unfortunately of too poor quality to be used, probably due to fragmented DNA or contaminations.
Reporter System
We proved that GFP is visible in horse feces and could be used as a reporter. We also improved a previous BioBrick of the UnaG chromoprotein for possible integration in our sensor.
Phage Display
Computational analysis of our phage display peptides showed two especially promising peptides:
- EF01122224: TPIFLPTPAQEH
- EF01122218: FSPTQANTIHRW
Worm Culturing
We successfully sterilized and cocultured cyathostominae with E.coli as well as creating a microfluidic chip for easier separation of large and small strongyles