Difference between revisions of "Team:UPF CRG Barcelona/Intermediate"
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which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a weak RBS (BBa_B0032). | which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a weak RBS (BBa_B0032). | ||
</p> | </p> | ||
| − | <center><img src="/ | + | <center><img src="https://static.igem.org/mediawiki/2018/7/75/T--UPF_CRG_Barcelona--intermediateconstruction_1.svg" style="width: 35%;"></center> |
<p><b>BBa_K2581001: T14_pfadBA3</b></p> | <p><b>BBa_K2581001: T14_pfadBA3</b></p> | ||
<p>This is the construction intermediate used in the BBa_K2581005 part. It consists of the assembly of a double | <p>This is the construction intermediate used in the BBa_K2581005 part. It consists of the assembly of a double | ||
terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter | terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter | ||
which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a weak RBS (BBa_B0033).</p> | which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a weak RBS (BBa_B0033).</p> | ||
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<p><b>BBa_K2581002: T14_pfadBA4</b></p> | <p><b>BBa_K2581002: T14_pfadBA4</b></p> | ||
<p>This is the construction intermediate used in the BBa_K2581006 part. It consists of the assembly of a double | <p>This is the construction intermediate used in the BBa_K2581006 part. It consists of the assembly of a double | ||
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(BBa_B0034). | (BBa_B0034). | ||
</p> | </p> | ||
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<p><b>BBa_K2581003: 32_fadD</b></p> | <p><b>BBa_K2581003: 32_fadD</b></p> | ||
<p>This is the construction intermediate used in the BBa_K2581009 part. It consists in the assembly of the weak | <p>This is the construction intermediate used in the BBa_K2581009 part. It consists in the assembly of the weak | ||
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genome. | genome. | ||
</p> | </p> | ||
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<p><b>BBa_K2581013: T14_pAR</b></p> | <p><b>BBa_K2581013: T14_pAR</b></p> | ||
<p>The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty | <p>The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty | ||
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promoter based on the previous pFadBA DNA sequence. | promoter based on the previous pFadBA DNA sequence. | ||
</p> | </p> | ||
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<p><b>BBa_K2581017: T14_pFadBA4_CI_T14_prm4_RFP or Intermediate fatty acid biosensor with sharp response | <p><b>BBa_K2581017: T14_pFadBA4_CI_T14_prm4_RFP or Intermediate fatty acid biosensor with sharp response | ||
</b></p> | </b></p> | ||
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(BBa_E1010). | (BBa_E1010). | ||
</p> | </p> | ||
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<p><b>BBa_K2581018: T14_pFadBA2_CI_T14_prm2_RFP or Intermediate fatty acid biosensor with sharp response | <p><b>BBa_K2581018: T14_pFadBA2_CI_T14_prm2_RFP or Intermediate fatty acid biosensor with sharp response | ||
</b></p> | </b></p> | ||
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(BBa_B0032), and an engineered mutant of red fluorescent protein from <i>Discosoma striata</i> (BBa_E1010). | (BBa_B0032), and an engineered mutant of red fluorescent protein from <i>Discosoma striata</i> (BBa_E1010). | ||
</p> | </p> | ||
| − | <center><img src="/ | + | <center><img src="https://static.igem.org/mediawiki/2018/d/de/T--UPF_CRG_Barcelona--highpassfilter32.svg" alt="T14_pAR part" style="width: 55%"></center> |
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Revision as of 22:29, 16 October 2018
CONSTRUCTION INTERMEDIATES
Bba_K2581000: T14_pfadBA2
It is the construction intermediate used in the BBa_K2581004 part. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a weak RBS (BBa_B0032).
BBa_K2581001: T14_pfadBA3
This is the construction intermediate used in the BBa_K2581005 part. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a weak RBS (BBa_B0033).
BBa_K2581002: T14_pfadBA4
This is the construction intermediate used in the BBa_K2581006 part. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), and a strong RBS (BBa_B0034).
BBa_K2581003: 32_fadD
This is the construction intermediate used in the BBa_K2581009 part. It consists in the assembly of the weak RBS 32 (BBa_B0032) and the FadD gene (BBa_K2581016) obtained of the own E. coli DH5-alpha strain genome.
BBa_K2581013: T14_pAR
The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_k817002). This new promoter has two binding sites for FadR in comparison with the existing part. Therefore, we have demonstrate an enhanced function of this promoter resulting in a disminution of basility and hence, an improvement of this part. This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), and the improved promoter based on the previous pFadBA DNA sequence.
BBa_K2581017: T14_pFadBA4_CI_T14_prm4_RFP or Intermediate fatty acid biosensor with sharp response
The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake consisting in a high pass filter in order to reduce the high basal activity of the promoter in another efficient way. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), a strong RBS (BBa_B0034), a CI repressor from E. coli phage lambda (+ LVA) (BBa_C0051), again the double terminator (BBa_B0014), a modified lambda Prm promoter (BBa_I12036), again the strong RBS (BBa_B0034) and an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
BBa_K2581018: T14_pFadBA2_CI_T14_prm2_RFP or Intermediate fatty acid biosensor with sharp response
The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake consisting in a high pass filter in order to reduce the high basal activity of the promoter in another efficient way. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_K817002), a weak RBS (BBa_B0032), a CI repressor from E. coli phage lambda (+LVA) (BBa_C0051), again the double terminator (BBa_B0014), a modified lambda Prm promoter (BBa_I12036), again the weak RBS (BBa_B0032), and an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).
