Difference between revisions of "Team:Edinburgh OG/Improve"
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<td>The phasin autoregulation system </td> | <td>The phasin autoregulation system </td> | ||
<td>552bp</td> | <td>552bp</td> | ||
| − | <td> Source of sequence :<a href="http://mic.microbiologyresearch.org/content/journal/micro/10.1099/00221287-148-8-2413#tab2">Regulation of phasin expression and polyhydroxyalkanoate (PHA) granule formation in Ralstonia eutropha H16 (2002)</a> and NCBI (No further indication). No codon optimisation for <em>E.coli</em>.</td> | + | <td> Source of sequence :<a href="http://mic.microbiologyresearch.org/content/journal/micro/10.1099/00221287-148-8-2413#tab2">Regulation of phasin expression and polyhydroxyalkanoate (PHA) granule formation in Ralstonia eutropha H16 (2002)</a> and NCBI (No further indication). No codon optimisation for <em>E.coli</em>. No result or user experience provided. </td> |
</tr> | </tr> | ||
</tbody> | </tbody> | ||
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<td>The native promoter and RBS of phasin autoregulation system </td> | <td>The native promoter and RBS of phasin autoregulation system </td> | ||
<td>234bp</td> | <td>234bp</td> | ||
| − | <td> Source of sequence :<a href="http://mic.microbiologyresearch.org/content/journal/micro/10.1099/00221287-148-8-2413#tab2">Regulation of phasin expression and polyhydroxyalkanoate (PHA) granule formation in Ralstonia eutropha H16 (2002)</a> and NCBI (No further indication). No codon optimisation for <em>E.coli</em>.</td> | + | <td> Source of sequence :<a href="http://mic.microbiologyresearch.org/content/journal/micro/10.1099/00221287-148-8-2413#tab2">Regulation of phasin expression and polyhydroxyalkanoate (PHA) granule formation in Ralstonia eutropha H16 (2002)</a> and NCBI (No further indication). No codon optimisation for <em>E.coli</em>. No result or user experience provided. </td> |
</tr> | </tr> | ||
</tbody> | </tbody> | ||
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<div class="row"> | <div class="row"> | ||
| − | <div class="container"><button class="btn btn-warning" type="button" data-toggle="collapse" data-target="#tittle4"><h3><strong>   Sequence Selection and Optimisation </strong></h3></button> | + | <div class="container"><button class="btn btn-warning" type="button" data-toggle="collapse" data-target="#tittle4"><h3><strong>   Sequence Selection and Codon Optimisation </strong></h3></button> |
<div id="tittle4" class="collapse"> | <div id="tittle4" class="collapse"> | ||
<div class="col-sm-5"> | <div class="col-sm-5"> | ||
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<blockquote> | <blockquote> | ||
| − | <p style="text-align: justify;">The sequence of PhaR promoter is more properly selected in comparison with the previous parts. Previous publication: <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3570403/">Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate] (2013) </a> is served as reference guideline and the selected functional promoter sequence is 134bp shorter than the previous part.</p> | + | <p style="text-align: justify;">The sequence of PhaR promoter in this project is more properly selected in comparison with the previous parts. Previous publication: <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3570403/">Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate] (2013) </a> is served as reference guideline and the selected functional promoter sequence is 134bp shorter than the previous part.</p> |
| − | <p style="text-align: justify;">The template sequence of PhaR is based on <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a>, being 4bp difference to the previous part. </p> | + | <p style="text-align: justify;">The template sequence of PhaR in this project is based on <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a>, being 4bp difference to the previous part. </p> |
<left><img src="https://static.igem.org/mediawiki/parts/8/88/T--Edinburgh_OG--Improve-image_2.jpeg" height="auto"></left> | <left><img src="https://static.igem.org/mediawiki/parts/8/88/T--Edinburgh_OG--Improve-image_2.jpeg" height="auto"></left> | ||
<p style="text-align: justify;">Figure 1. Sequence alignment of NCBI(AM260479.1) and BBa_K108015. The 4bp difference is indicated by red color. </p> | <p style="text-align: justify;">Figure 1. Sequence alignment of NCBI(AM260479.1) and BBa_K108015. The 4bp difference is indicated by red color. </p> | ||
Latest revision as of 23:49, 16 October 2018
