Owen-lo 923 (Talk | contribs) |
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− | <div class="container"><button class="btn btn-warning" type="button" data-toggle="collapse" data-target="#tittle4"><h3><strong>   Sequence Selection and Optimisation </strong></h3></button> | + | <div class="container"><button class="btn btn-warning" type="button" data-toggle="collapse" data-target="#tittle4"><h3><strong>   Sequence Selection and Codon Optimisation </strong></h3></button> |
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− | <p style="text-align: justify;">The sequence of PhaR promoter is more properly selected in comparison with the previous parts. Previous publication: <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3570403/">Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate] (2013) </a> is served as reference guideline and the selected functional promoter sequence is 134bp shorter than the previous part.</p> | + | <p style="text-align: justify;">The sequence of PhaR promoter in this project is more properly selected in comparison with the previous parts. Previous publication: <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3570403/">Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate] (2013) </a> is served as reference guideline and the selected functional promoter sequence is 134bp shorter than the previous part.</p> |
<p style="text-align: justify;">The template sequence of PhaR in this project is based on <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a>, being 4bp difference to the previous part. </p> | <p style="text-align: justify;">The template sequence of PhaR in this project is based on <a href="https://www.ncbi.nlm.nih.gov/nuccore/AM260479.1/">NCBI(AM260479.1)</a>, being 4bp difference to the previous part. </p> | ||
<left><img src="https://static.igem.org/mediawiki/parts/8/88/T--Edinburgh_OG--Improve-image_2.jpeg" height="auto"></left> | <left><img src="https://static.igem.org/mediawiki/parts/8/88/T--Edinburgh_OG--Improve-image_2.jpeg" height="auto"></left> |
Latest revision as of 23:49, 16 October 2018