Difference between revisions of "Team:BCU/Description"

 
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{{BCU}}
 
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<h1>Description</h1>
 
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<p>                </p>
 
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<h4>1</h4>
 
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<h4>Nicotine (1-methyl-2-[3-pyridyl-pyrrolidine], C10H14N2), a major alkaloid in tobacco plants, is a significant factor of evaluation for tobacco and cigarettes. Nicotine plays a critical role in smoking addiction and is well known to be harmful to human beings, because it easily crosses the blood-brain barrier and biological membranes. Meanwhile, with large quantities of tobacco products being produced and consumed, tobacco waste, which includes many toxic substances such as nicotine, aminobiphenyl, naphthylamine, and benzo(a)- pyrene, is entering the environment. Moreover, a number of toxic and harmful substances containing nicotine and other alkaloids are generated in tobacco processing and cannot be recycled, producing serious environmental problems.
 
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<img src="https://static.igem.org/mediawiki/2018/8/88/T--BCU--navpic.jpg">
 
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<h4>2</h4>
 
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<h4>In recent years, more nicotine-degrading bacteria have been reported, and the degradation pathways of nicotine have been elucidated. The degradation of nicotine through the pyrrolidine pathway by Pseudomonas has been extensively studied.
 
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</h4>
 
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<img src="https://static.igem.org/mediawiki/2018/e/eb/T--BCU--describe1-1.png">
 
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<h4>3</h4>
 
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<h4>In our study， the first three gene NicA, pnao and sapd is crucial for nicotine degradation, so we will construct a pathway to express the three gene in top10.
 
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</h4>
 
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<img src="https://static.igem.org/mediawiki/2018/1/18/T--BCU--describe2-1.png">
 
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<hr></hr>
 
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<h4>Nicotine degradation</h4>
 
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<h4>Gene cloning</h4>
 
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<h4>Pathway construction</h4>
 
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<h4>Vector construction</h4>
 
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<h4>Protein expression</h4>
 
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<h4>Screening</h4>
 
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<h4>Nicotine catalysis</h4>
 
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<div class="button_link"> <a href="https://2018.igem.org/Team:BCU/Description"> Back to the top </a>
 
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<span><a  href="https://2018.igem.org/Team:BCU">HOME</a></span>
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<span><a  href="https://2018.igem.org/Team:BCU/Team">TEAM</a></span>
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<span><a href="https://2018.igem.org/Team:BCU/Description">PROJECT</a></span>
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<span>EXPERIMENT</span>
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<p><a href="https://2018.igem.org/Team:BCU/Design">Design</a></p>
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<p><a href="https://2018.igem.org/Team:BCU/Improve">Materials&Reagents</a></p>
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<p><a href="https://2018.igem.org/Team:BCU/Demonstrate">Methods</a></p>
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<p><a href="https://2018.igem.org/Team:BCU/Results">Results</a></p>
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<span><a href="https://2018.igem.org/Team:BCU/Parts">PARTS</a></span>
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<span><a  href="https://2018.igem.org/Team:BCU/InterLab">INTERLAB</a></span>
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<span><a  href="https://2018.igem.org/Team:BCU/Notebook">NOTEBOOK</a></span>
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<span><a  href="https://2018.igem.org/Team:BCU/Attributions">ATTRIBUTION</a></span>
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<span><a  href="https://2018.igem.org/Team:BCU/Model">MEDAL</a></span>
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<div class="main">
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<h1>PROJECT</h1>
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                <img src="https://static.igem.org/mediawiki/2018/3/3b/T--BCU--PROJECT08.jpg">
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<img src="https://static.igem.org/mediawiki/2018/4/40/T--BCU--xiabianlan02.jpg">
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Latest revision as of 01:11, 17 October 2018

无标题文档

HOME
TEAM
PROJECT
EXPERIMENT

Design

Materials&Reagents

Methods

Results

PARTS
INTERLAB
NOTEBOOK
ATTRIBUTION
MEDAL

PROJECT