Difference between revisions of "Team:SHSID China/Notebook"

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         <h2 style="color: white; font-family: 'Trocchi', serif;">Lab Notes</h2>
 
         <h2 style="color: white; font-family: 'Trocchi', serif;">Lab Notes</h2>
 
         <p style="color: white; font-size: 16px; padding-top: 10px">[Abbreviation notes: 4L = part BBa_K325909 (LuxCDABEG), 2D = part BBa_K325219 (Red firefly luciferase and LRE), 4N = part BBa_K325100 (EPIC firefly luciferase and LRE); Concentration of plasmids are in units of ng/μL (if not mentioned)]</p>
 
         <p style="color: white; font-size: 16px; padding-top: 10px">[Abbreviation notes: 4L = part BBa_K325909 (LuxCDABEG), 2D = part BBa_K325219 (Red firefly luciferase and LRE), 4N = part BBa_K325100 (EPIC firefly luciferase and LRE); Concentration of plasmids are in units of ng/μL (if not mentioned)]</p>
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         <h3 style="color: white; font-family: 'Trocchi', serif; text-align: center">Lab Notes</h3>
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         <h3 style="color: white; font-family: 'Trocchi', serif; text-align: center">July</h3>
 
         <h4 style="color: white; font-family: 'Trocchi', serif;"><strong>7/17</strong></h4>
 
         <h4 style="color: white; font-family: 'Trocchi', serif;"><strong>7/17</strong></h4>
 
         <ul style="color: white; font-size: 16px; padding-top: 10px">
 
         <ul style="color: white; font-size: 16px; padding-top: 10px">
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                     <li>Transformed 2*LuxG, 2*4L, 2*2D, 2*4N, totaling up to 9 petri dishes</li>
 
                     <li>Transformed 2*LuxG, 2*4L, 2*2D, 2*4N, totaling up to 9 petri dishes</li>
 
                     <li>2*LuxG 2*4L 1*2D 1*4N totaling up to 6 test tubes</li>   
 
                     <li>2*LuxG 2*4L 1*2D 1*4N totaling up to 6 test tubes</li>   
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        </ul>
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        <h4 style="color: white; font-family: 'Trocchi', serif;"><strong>7/26</strong></h4>
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        <ul style="color: white; font-size: 16px; padding-top: 10px">
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                    <li>PCR LuxG with primers</li>
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                    <li>Added 4L monoclone into 2 Erlenmeyer flasks</li>
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                    <li>Used XBaI and PstI to digest the 4L monoclone</li>
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                    <li>Extracted plasmids (1*4N, 1*2D, 2*4L, 2*LuxG)</li>
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                    <li>Gel electrophoresis the products from PCR and enzyme digestion.</li>
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        </ul>
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        <h4 style="color: white; font-family: 'Trocchi', serif;"><strong>7/27</strong></h4>
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        <ul style="color: white; font-size: 16px; padding-top: 10px">
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                    <li>LuxG gel extraction (digested by XBaI and PstI)</li>
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                    <li>Tested for concentration</li> 
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        </ul>
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        <h4 style="color: white; font-family: 'Trocchi', serif;"><strong>7/28</strong></h4>
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        <ul style="color: white; font-size: 16px; padding-top: 10px">
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                    <li>Took 20ml of 4L from the Erlenmeyer flask</li>
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                    <li>Extracted plasmid (4*2D, 3*4L)</li>
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                    <li>Conducted gel electrophoresis to confirm plasmid size</li>
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                    <li>Tested for concentration</li>
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                    <li>4L succeeded, but the concentration of 2D was too low for gel electrophoresis</li>
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                    <li>Added 8mL of 0.1M Arabinose into a 80mL flask and 10ml of 0.1 Arabinose into a100mL flask</li>
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                    <li>After 3.5 hours, visible light was emitted</li>
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                    <li>Gradient test of 4L with 0.1M Arabinose</li>
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        </ul>
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        <h4 style="color: white; font-family: 'Trocchi', serif;"><strong>7/29</strong></h4>
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        <ul style="color: white; font-size: 16px; padding-top: 10px">
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                    <li>Prepared 3L of LB, but had to be redone due to an inaccurate electronic balance</li>
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                    <li>Prepared 2L liquid LB, 1 L solid LB. All sterilized.</li>
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                    <li>Washed and sterilized 62 test tubes.</li>
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                    <li>Organized plasmids and DNA</li>
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                    <li>Designed further experiments with guidance professors.</li>
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        </ul>
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        <h3 style="color: white; font-family: 'Trocchi', serif; text-align: center">August</h3>
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        <h4 style="color: white; font-family: 'Trocchi', serif;"><strong>8/1</strong></h4>
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        <ul style="color: white; font-size: 16px; padding-top: 10px">
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                    <li>Extracted 8 tubes of pHB plasmid</li>
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                    <li>Concentration: 67.439, 72.8, unknown, 128.6, 203.2, 153.6, 101.2, 61.7</li>
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                    <li>Digested pHB using BamHI and PstI, ran gel electrophoresis and did gel extraction</li>
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                    <li>Digested 4L using BamHI and PstI, ran gel electrophoresis and did gel extraction</li>
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                    <li>Monoclone 4L into 2 flasks and 4 test tubes</li>
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                    <li>Gradient test</li>
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                    <li>0.1M solution emitted the brightest light.</li>
 
         </ul>
 
         </ul>
 
     </article>
 
     </article>

Revision as of 02:21, 17 October 2018

Notebook

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